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OBJECTIVE Synthesize flavone and flavonone ,and test their inhibition activity towards aldose reductase.

目的对采用化学方法合成的黄酮及二氢黄酮母体对醛糖还原酶的抑制活性进行测试。

The pigment in colored cotton fibers was extracted by methanol at room temperature. The results of color reactions HCl-Mg reaction, NaBH4 reaction, NaOH reaction, FeCl3 reaction and Pb(Ac2 reaction of the pigment extractive showed that the pigment extracted by methanol was flavone or flavonol with two adjacent hydroxide groups (Table 2, Fig. 1). The results of UV spectral analysis further proved that the pigment extracted by methanol from brown fiber belonged to flavone with two adjacent hydroxide groups in B ring, and that from the green fiber contained not only flavone but also flavonols with two adjacent hydroxide groups both in A ring and B ring and indican replaced a hydroxide group at 3-site of A ring Fig.

通过盐酸-镁粉反应、四氢硼钠还原反应、碱反应、醋酸铅反应、氯化铁反应和紫外光谱吸收的分析表明,甲醇室温提取的棕色棉纤维色素可能是黄酮类化合物,B环上带有邻二酚羟基;甲醇室温提取的绿色棉纤维色素除黄酮类化合物外,还含有3-位羟基被糖甙化的黄酮醇类化合物,A、B环上都带有邻二酚羟基。

Mucopolysaccharide was a kind of natural molecular, which was basically consisted of aldoses linked by indicant bond, and was almost found in all organisms including animal, plant and microbe.

粘多糖是由糖苷键连接起来的醛糖或酮糖组成的天然分子,几乎存在于所有的有机体中,包括动物、植物、微生物。

However every kind of sugar isdifferent structure, for example four -OH of galactose is 1a2a3a4a, glucose is 1a2a3a4e;Xylose is a five-ring ketose and glucose is six-ring aldose.

葡萄糖与半乳糖相比其构象不同:半乳糖的四个—OH为1a2a3a4a,而葡萄糖是1a2a3a4e;同时与六元醛糖的葡萄糖相比,木糖是五元酮糖。

Nine new compounds were characterized, and they were: opuntioside Ⅰ(11), 4-ethoxyl-6-hydroxymethyl-α-pyrone (14),--ester (22), 1-butoxyl--malate (24), ethyl α-L-rhamnopyranoside (26), opuntioside Ⅱ(34), kaempferol 7-O-β-D-glucopyranosyl (1→4)-β-D-glucopyranoside (46), methyl α-D-glucopyranoside-6-(methyl 4-malate)-ester (47), and opuntioside Ⅲ(48). Opuntioside Ⅰ,Ⅱ,Ⅲ and 4-ethoxyl-6-hydroxymethyl-α-pyrone belong to the skelecton of α-pyrone.

新化合物分别是:仙人掌苷Ⅰ(11),4-乙氧基-6-羟甲基-α-吡喃酮(14),琥珀酸乙酯--酯(22),L--苹果酸-1-丁酯(24),乙基α-L-吡喃鼠李糖苷(26),仙人掌苷Ⅱ(34),山奈酚7-O-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃葡萄糖苷(46),α-D-吡喃葡萄糖甲苷-6-(苹果酸-4-甲酯)-酯(47),仙人掌苷Ⅲ(48),其中仙人掌苷Ⅰ、Ⅱ、Ⅲ及4-乙氧基-6-羟甲基-α-吡喃酮均为α-吡喃酮骨架类型的新化合物。

This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻糖负载红细胞的影响,在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时,用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率,用硫酸蒽酮法检测红细胞内海藻糖浓度水平。

Water,butylene glycol,biosaccharide gum-1,ppg-26-buteth-26,panthenol,peg-40 hydrogenated caster oil,hydroxyethylcellulose,imidazolidinyl urea,saccharide isomerate,benzophenone-4,phenoxyethanol,triethanolamine,citrus aurantium dulcisoil,pentadecalactone,methylparaben,sodium hyaluronate,citric acid,butylparaben,ethylparaben,citrus aurantium amaraoil,propylparaben,methylchloroisothiazolinone,methylisothiazolinone,red 4,yellow 5,red 33,limonene,linalool

水,丁二醇,多醣物质,PPG-26-丁醇聚醚-26,维生素B5,PEG-40氢化蓖麻油,羟乙基纤维素,尿素醛,天然异构寡糖,二苯甲酮- 4,苯氧乙醇,三乙醇胺,甜橙油,环十五内酯,羟苯甲酯,透明质酸钠,柠檬酸,羟苯丁酯,羟苯乙酯,苦橙油,羟苯丙酯,甲基氯异塞唑晽酮,甲基异噻唑啉酮,红4,黄5,红33,柠檬精油,芳樟醇

Green tea contains a number of flavonols with the basic structure Specific flavonols found in tea include myricetin (hydroxyls at carbon 3', 4', and 5'), quercetin (hydroxyls at positions 3' and 4'), and kaempferol (hydroxyl at position 4'), and their glycosides.

绿茶含有一些黄酮醇,其基本化学结构为:茶中特有的黄酮醇有杨梅黄酮(3'、4'、5'碳位上羟基化)、豕草花粉苷(3'和4'碳位上羟基化),莰非醇(4'碳位上羟基化),以及它们的糖苷物。

The pentose s include four keto pentose ,four pentitol and eight aldo pentose .

戊糖类物质包含4种戊酮糖、4种戊糖醇和8种戊醛糖,它们在膳食、保健、医药等领域中发挥着重要的功能。

The quality of purified 2-KGA from its fermentation products was up to industrial standard. In addition, several hexoses, such as D-glucose and D-sorbitol can be utilized to synthesize 2-KGA by this newly-mixed culture. The growth and anabolism of the B529-V6 were studied in 4M〓 and 300M〓 fermentor respectively. The results showed that its growth and anabolism were affected by carbon sources, ratio of D-glucose and L-sorbose, nitrogen sources, growth factors, inoculum quality and culture condition including the initial pH value, temperature and ventilation.

新菌系B529-V6表现出了较强的高浓度L-山梨糖耐受能力、较高的底物代谢速率和较高的2-KGA转化能力,在8%山梨糖浓度的发酵培养基中培养48h,糖酸转化率较对照菌系提高了5.94%;山梨糖浓度提高至10%,其生长代谢受影响程度较小,且能不同程度地利用葡萄糖和山梨醇为底物,合成维生素C前体-2-酮基-L-古龙酸(2-KGA),其发酵产物2-KGA经反相高效液相色谱分析其质量符合工业化生产要求。

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