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To enhance the efficiency of cellulose bio-degradation and reduce the mass transfer resistance from cellulose consequently increase the extraction rate of diosgenin, glucose was separated andβ-glycosidase was added in to decompose cellobiose in the biocatalysis pretreatment.

为了提高纤维素酶的作用效率,降低薯蓣皂苷元提取过程中纤维素带来的传质阻力,从而提高薯蓣皂苷元产率,在酶解的预处理过程中对糖液进行分离并添加β-葡萄糖苷酶分解体系中的纤维二糖。

The HPLC methods for analysis of N--ribitylxylidine and N-methyl glucamine were established. After optimizing the operation conditions, the principal product and main impurities were well separated. And they were determined by LC-MS. The operation condition of N--ribitylxylidine is, columniation temperature is 30℃, flow rate is 0.7mL/min, UV wave is 244nm, the mobile phase is methanol: water=40: 60 .

首次建立了对葡甲胺和核糖胺的高效液相色谱分析方法,通过对色谱操作条件的优化,成功分离了主产物和杂质,在此条件下,应用液相色谱-质谱联用仪对糖基胺及生成杂质进行了指派定性,并测定了两种糖基胺及其主要杂质的含量,获得了满意的结果。

RESULTS: ADMSCs at passage 3 were positive for CD13, CD59 and CD44, but negative for CD34 and HLA-DR. Chromosomal morphous, structure and number were normal, showing diplont structure. Under the microscope, there were complete Golgi complex, plenty of rough endoplasmic reticulum, and some glycogen granules in cytoplasm.

结果:第3代脂肪间充质干细胞CD13,CD44,CD59均呈阳性表达,而造血干细胞表面标志CD34及成纤维细胞表面标志HLA-DR均呈阴性表达;染色体的形态、结构和数目均未见异常,呈二倍体结构;镜下可见完整的高尔基复合体,数量较多的粗面内质网,部分糖元颗粒分布在胞浆中。

After the pertinence analysis of endoplasm parameters, designed an artificial neural network which built the training sample with grape images information and pericarpial pigment parameters and another artificial neural network which built the training samples with pericarpial pigment parameters and total acid, total sugar, soluble solids of sarcocarp. Using this combined module, realized the nondestructive measurement according to grape images information.

通过对内质参数的相关性分析,设计了由葡萄图像信息预测色素内质参数的人工神经网络及由果皮色素参数预测果肉总酸、总糖、可溶性固形物的人工神经网络模型,通过两个模型组合来实现根据葡萄图像信息进行采前无损检测的目的。

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段,对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验,对于质粒在硫杆菌中普遍存在的观点提出了质疑。

So far this method has been successfully applied to HPLC analysis of simple monosaccharides. A baseline separation of five monosaccharides was obtained under optimized isocratic condition. Both UV detector and electrospray ionization-Mass spectrometry were selected to determine the monosaccharide composition in real samples.

结合建立的衍生化方法与高效液相色谱HPLC,优化分离条件,并选择紫外和电喷雾—离子阱质谱两种检测模式对实际样品中所含的单糖进行分离分析,建立了5种单糖的常规定性定量方法。

After dipyridamole was bound to the cell membrane, the test sample was obtained by ultrasonically dissolving the labeled membrane in 10mmol L〓 tris-HCl membrane lyses solution.

氨基酸、糖、药物、肽、核苷、离子和神经递质等物质不易通过细胞膜的脂质双层,而必须通过细胞膜上的载体蛋白转运体协助才能进入细胞。

For example, significant correlation in Zunyi prefecture was found between smoking total scores and total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor and total nitrogen and chloride.

在省级尺度,烟叶化学成分与烟叶品质的关系更为复杂,需用更多的化学成分(碳水化合物、含氮化合物及矿质养分)才能综合反映烟叶的品质,如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关,杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

At the prefecture scale, the relationship became complicated between chemical components and leaf quality, and more chemical components were needed to represent leaf quality. For example, significant correlation in Zunyi prefecture was found between smoking total scores with total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor with total nitrogen and chloride.

在省级尺度,烟叶化学成分与烟叶品质的关系更为复杂,需用更多的化学成分(碳水化合物、含氮化合物及矿质养分)才能综合反映烟叶的品质,如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关,杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

Transport of oxaloacetate Oxaloacetate, the product of the first step in gluconeogenesis, must leave the mitochondrion and enter the cytosol where the subsequent enzyme steps take place.

草酰乙酸的运输在糖异生作用中,第一部产物草酰乙酸必须离开线粒体并进入胞质溶胶,在胞质溶胶中进行以后的酶促反应步骤。

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