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Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制,还采用国内外经典细胞培养方法,成功地培养出肾小球系膜细胞,在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生,结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成,证明其对防止肾小球硬化有明显作用。

Glucosidase deficiency disease is a rare genetic disorder in which the lysosomal enzyme α-glucosidase is markedly reduced and leads to accumulation of glycogen in various tissues. Its clinical features include massive enlargement of the internal organs, skeletal muscle weakness and hypotonia, and respiratory insufficiency.

阿法-糖苷酶缺乏之全身性糖原质病是一种少见的遗传性疾病,此病主要是因为缺乏阿法-糖苷酶而造成过多的糖原质堆积在各种组识中,导效患者在临床上会有内脏扩大、肌肉无力和呼吸不足等症状。

Once the cells reached fluences of FBS coating on adhesive rats of MSCs: Some of the purified HUCB MSCs at logarithmic phase were growth curve of MSCs: The growth of cells were observed by phase contrast microscope (OLYMPUS CK40), and were detected by EPICS-ALTRA flow cytometry.

免疫表型:取扩增第5代的脐血间充质干细胞,用EPICS-ALTRA流式细胞仪进行检测。主要观察指标:①观察高糖组和低糖组细胞的生长状态。②观察不同时间点胎牛血清包被组与未包被组细胞的贴壁情况并分别计算贴壁率。结果:本实验总共培养和分析了70例脐血间充质干细胞样本。

The Tg for a mass ratio mixture of Galpi and sucrose of 0.3/1 was greater than 0 and was similar to that of the same ratio mixture of raffinose to sucrose, indicating that Galpi as well as oligosaccharides plays an important role in glass formation.

甲基肌醇半乳吡喃糖和蔗糖以0.3对1重量浓度混合比例,其玻璃质化转移温度在0℃以上,且近似於相同比例之寡糖类和蔗糖混合,显示甲基肌醇半乳吡喃糖和寡糖类在玻璃质化形成上扮演重要的角色。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya,以绿色荧光蛋白基因为报告基因,构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46,荧光显微镜检测发现,此启动子在两菌株中都具有功能。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质粒的启动子在糖多孢红霉菌中都不能发挥作用,故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子,并利用pSET152质粒上链霉菌染色体整合位点和氨朴霉素抗性基因,构建了表达载体pZMW。

Staining observation on complex mesochondrium of marrow-derived mesenchymal stem cells-TCP: It was found with TB staining that the complex of marrow-derived mesenchymal stem cells-TCP inside the joint cavity obviously had positive staining, which indicated that there were glycosidoprotein base formed.

骨髓基质干细胞-磷酸三钙复合体软骨基质特染观察结果:甲苯胺蓝染色发现,植入关节腔的骨髓基质干细胞-磷酸三钙复合体有明显阳性染色,表明有糖蛋白基质形成。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中,DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F,以重组质粒DNA和脂质体共转染COS-7细胞,用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

The treatment comparison by using Chinese TANG Kidney capsule and Western medicine TANG SHI PIN showed: the kidney capsule can lower the values of the blood sugar, blood fat, series of micro-protein un urine and carbon monoxide in plasma, thus the function of kidney can be improved, and prolong the process of deadness and hardening of the affected kidney small cell fibre.

利用该模型选择中药糖肾胶囊与西药糖适平对照治疗研究,结果显示:糖肾胶囊能降低糖尿病肾病大鼠的血糖、血脂、尿素氮、肌酐、尿系列微量蛋白及血一氧化氮等指标,从而减轻糖尿病肾病大鼠肾小球的高灌注高滤过,降低糖化血红蛋白含量,减轻非酶糖基化,改善肾功能,降低血脂、内皮素、调节血栓素、前列腺素水平,有抗脂质过氧化损伤的作用。

This technique is the hardhitting technique of currently International, some companies-made sugar of Japan and Europe take the xylitol as matrix , but not appending any composition.

这项技术可称得上是目前国际上糖醇类硬糖的高精尖技术,日本和欧洲的制糖企业有以木糖醇为基质生产的硬糖,但硬糖中没有添加任何成分。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。