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Supplied with different forms of carbon compounds to the bacteroid suspension as respiratory substrates investigated in Warburg respirometer , it showed that sucrose was nonutilizable and its hydrolysates , glucose and fructose were util .

试验以多种碳源供类菌体作呼吸基质,果表明蔗糖不能被利用,水解成葡葡糖和果糖后的利用率低。

They can be made more durable and stabilized by the addition of more sugar, gelatin, glycerine, glucose and Carrageen.

可以利用较多的糖、胶质、甘油、葡萄糖和角叉菜来增加持久和稳定性。

These results showed that the membrane glycoproteins were modified in the epididymis,which is essential for spermatozoa to participate in the process of fertilization.

这说明精子质膜糖蛋白在附睾中不断地被修饰,为精子能完成正常的受精过程做准备。

Under no circumstances may recipes be used for these two types of desserts which consists only of sugar, milk, colour and gelatine.

在任何情况之下,都不可以只用糖、奶、色素和胶质制作这二类型的餐后甜点。

This hormonal milieu increases glycogenolysis and gluconeogenesis, causes a net breakdown of skeletal muscle, and enhances lipolysis, which together provide endogenous glucose, amino acids, and free fatty acids that are required for cellular and organ function and wound healing.

这种激素环境增加糖原分解和糖异生,导致骨骼肌净分解,并且增加脂质分解,它们共同提供细胞和器官发挥功能以及创伤愈合所需的内源性葡萄糖、氨基酸和游离脂肪酸。

ConclusionsExpression of glucocorticoid receptor alpha was detected in the nuclei and cytoplasm in facial nucleus and peripheral lymph cells and was increased after facial nerve injuries. Local inflammatory reaction triggered by nerve injuries lasted for a longer duration than that caused by mere traumas.

h后。结论糖皮质激素受体α表达在面神经核团及外周淋巴细胞胞核及胞质中,在面神经受到损伤后其表达量增高,而且神经损伤较单纯外伤可以引起较长时间的局部炎症反应。

Now, pyronaridine bar food like the people of Tangshan has become the unique taste, CU Liu pyronaridine root, root pyronaridine braise, deep-fried bar pyronaridine, Jiao pyronaridine slide bar, glass bar pyronaridine, speculation pyronaridine root, root sign pyronaridine son, sweet and sour pyronaridine bar, etc.

现在,咯扎菜肴已经成为唐山人民喜爱的独特美味,醋溜咯扎、烩咯扎、炸咯扎、焦溜咯扎,玻璃咯扎,炒咯扎,咯扎签子、糖醋咯扎等等,据说能做一百多道菜,很有特色,摆在餐桌上,质高形异,色美味香。

Other features of the peripheral red blood cells are protein and lipid dysglycosylation and endoplasmic reticulum double-membrane remnants.

另外的特征是外周红血细胞蛋白和脂质糖基化不良以及残留的双膜内质网,其它造血系统是正常的。

Analysis of nutritional components in pear fruits of different varieties showed that fructose, glucose and sucrose were predominant sugar in pear fruits, the content of which ranged from 3.40% to 9.24%, 0.21% to 2.72%, 0.10% to 1.69%, respectively; Content of titrable acid was the highest in Bayuehong Pear(0.286%) and lowest in Atago(0.078%); Content of Vitamin C was the highest in Beurre Bosc (9.50 mg/100g) and lowest in Lubaoshi Pear(1.89mg/100g); Content of protein ranged from 127 to 428 mg/100g, content of free amino acid ranged from 24.8 to 77.4 mg/100g; Contents of the same mineral element of different pear varieties were different.

不同梨品种果实营养成分含量的分析结果表明:果糖、葡萄糖和蔗糖是梨果实中的主要糖类,品种间三种糖的含量有较大差异,所测品种果实中三种糖的含量范围分别为:3.40%~9.24%、0.21%~2.72%、0.10%~1.69%;可滴定酸的含量以八月红最高(0.286%),爱宕最低(0.078%);维生素C的含量以博斯克最高(9.50 mg/100g),绿宝石最低(1.89mg/100g);不同品种间可溶性蛋白和氨基酸的含量差异较大,其含量范围分别为127~428mg/100g和77.4~24.8 mg/100g;不同品种间同一矿质元素的含量存在较大差异。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时,本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上,经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后,得到阳性重组质粒pET32a-σC;将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达,经SDS-PAGE和Western-blbtting检测分析,融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应;将融合表达的蛋白纯化后作为包被抗原,建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法,此方法中抗原的最佳包被浓度为5μg/ml、标准阳性血清的最适稀释倍数为1:40倍,用此方法对50份鸭血清样品进行检测,并与琼脂糖凝胶扩散试验检测抗体的法相比较,证明此ELISA方法具有良好的特异性和敏感性,本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

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