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The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒,带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子,载体大小9880bp。

The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1,2-FT活性的改变,采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂,特别是Ⅱ型寡糖的变化。

Liquor with uncooked materials is a kind of safe, hygeian and pure new technological liquor.

摘 要:回顾了生料糖化与发酵的研究历程,阐述了淀粉构造,生淀粉糖化机理,生料酒曲组分,生料酿酒和生料酒曲在大、小曲酒、黄酒生产方面的应用方法;以及与己酸菌、酯化酶共酵来提高酒质的技术措施;提出生料酒是一种安全卫生纯净的新工艺白酒。

So far this method has been successfully applied to HPLC analysis of simple monosaccharides. A baseline separation of five monosaccharides was obtained under optimized isocratic condition. Both UV detector and electrospray ionization-Mass spectrometry were selected to determine the monosaccharide composition in real samples.

结合建立的衍生化方法与高效液相色谱HPLC,优化分离条件,并选择紫外和电喷雾—离子阱质谱两种检测模式对实际样品中所含的单糖进行分离分析,建立了5种单糖的常规定性定量方法。

Analysis of nutritional components in pear fruits of different varieties showed that fructose, glucose and sucrose were predominant sugar in pear fruits, the content of which ranged from 3.40% to 9.24%, 0.21% to 2.72%, 0.10% to 1.69%, respectively; Content of titrable acid was the highest in Bayuehong Pear(0.286%) and lowest in Atago(0.078%); Content of Vitamin C was the highest in Beurre Bosc (9.50 mg/100g) and lowest in Lubaoshi Pear(1.89mg/100g); Content of protein ranged from 127 to 428 mg/100g, content of free amino acid ranged from 24.8 to 77.4 mg/100g; Contents of the same mineral element of different pear varieties were different.

不同梨品种果实营养成分含量的分析结果表明:果糖、葡萄糖和蔗糖是梨果实中的主要糖类,品种间三种糖的含量有较大差异,所测品种果实中三种糖的含量范围分别为:3.40%~9.24%、0.21%~2.72%、0.10%~1.69%;可滴定酸的含量以八月红最高(0.286%),爱宕最低(0.078%);维生素C的含量以博斯克最高(9.50 mg/100g),绿宝石最低(1.89mg/100g);不同品种间可溶性蛋白和氨基酸的含量差异较大,其含量范围分别为127~428mg/100g和77.4~24.8 mg/100g;不同品种间同一矿质元素的含量存在较大差异。

The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.

用高效毛细管电泳测定了重组人促红细胞生成素的微多相性、肽图,同时采用毛细管电泳质谱联用技术鉴定了部分非糖基化胰酶消解片段和O-126糖基化肽的结构。

The accumulation of biomass was positively correlated with initial reducing sugar concentration of the culture media The biomass ceased to increase after 16h of culture in liquidized WWWSI, but it remained vigorous growth up to 24h in saccharificated WWWSI.

生物量的积累与基质中还原糖浓度呈正相关,液化废水中培养16h后,生物量不再有明显的上升,液化再糖化的废水中则一直旺盛生长至24h,对此进行动力学分析,构建了可较好拟合白地霉在糖化的小麦淀粉废水中生长过程的动力学模型。

For example, significant correlation in Zunyi prefecture was found between smoking total scores and total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor and total nitrogen and chloride.

在省级尺度,烟叶化学成分与烟叶品质的关系更为复杂,需用更多的化学成分(碳水化合物、含氮化合物及矿质养分)才能综合反映烟叶的品质,如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关,杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

At the prefecture scale, the relationship became complicated between chemical components and leaf quality, and more chemical components were needed to represent leaf quality. For example, significant correlation in Zunyi prefecture was found between smoking total scores with total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor with total nitrogen and chloride.

在省级尺度,烟叶化学成分与烟叶品质的关系更为复杂,需用更多的化学成分(碳水化合物、含氮化合物及矿质养分)才能综合反映烟叶的品质,如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关,杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

Product of glycolysis is two three-carbon sugars, called pyruvates, and some ATP, which supplies energy to the yeast and allows it tomultiply.

糖酵系发生的是二个3碳糖,称为丙酮酸盐,和一些腺三磷,这些货色给酵母供给能质争其滋生。

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