糖质

Objective: The purpose of our studies are gaining a better insight into the effect of genistein on mesangial cell"s phenotype change and ECM expression plus degradation under DN circumstances.We add genistein with different concentrations in high glucose cultured rat mesangial cells;feed DM rats with the single concentration genistein for different days;to explore the expression of SMemb,the mark of mesangial cell"s hypertrophy phenotype change;to examine the synthesis of Fn as well as the change of the balance between MMP-2 and TIMP-l;to comprehend its effect on renal pathological changes of DM rats and some related factors;trying toexplore the possible mechanisms.Through these studies,we will have some information about the experimental value of genistein in DN field.

目的：为了研究金雀异黄素对DN环境下大鼠MC肥大表型转换标志物SMemb和ECM分泌及降解的影响，我们拟在细胞水平以不同浓度的金雀异黄素作用于高糖培养下大鼠MC，在动物水平以同一浓度的金雀异黄素作用于DM大鼠不同时间，观察细胞表型转换标志物SMemb、ECM主要成分Fn、ECM降解酶系统基质金属蛋白酶—2(MMP-2)／基质金属蛋白酶抑制剂—1(TIMP-1)表达的变化，同时观察金雀异黄素对DM大鼠肾脏病理及影响肾脏病进展的糖脂代谢紊乱的影响，并进一步探讨金雀异黄素发挥作用的可能机制；为明确金雀异黄素在DN治疗中的地位及意义提供实验依据。

Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制，还采用国内外经典细胞培养方法，成功地培养出肾小球系膜细胞，在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生，结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成，证明其对防止肾小球硬化有明显作用。

Glucosidase deficiency disease is a rare genetic disorder in which the lysosomal enzyme α-glucosidase is markedly reduced and leads to accumulation of glycogen in various tissues. Its clinical features include massive enlargement of the internal organs, skeletal muscle weakness and hypotonia, and respiratory insufficiency.

阿法-糖苷酶缺乏之全身性糖原质病是一种少见的遗传性疾病，此病主要是因为缺乏阿法-糖苷酶而造成过多的糖原质堆积在各种组识中，导效患者在临床上会有内脏扩大、肌肉无力和呼吸不足等症状。

Once the cells reached fluences of FBS coating on adhesive rats of MSCs: Some of the purified HUCB MSCs at logarithmic phase were growth curve of MSCs: The growth of cells were observed by phase contrast microscope (OLYMPUS CK40), and were detected by EPICS-ALTRA flow cytometry.

免疫表型：取扩增第5代的脐血间充质干细胞，用EPICS-ALTRA流式细胞仪进行检测。主要观察指标：①观察高糖组和低糖组细胞的生长状态。②观察不同时间点胎牛血清包被组与未包被组细胞的贴壁情况并分别计算贴壁率。结果：本实验总共培养和分析了70例脐血间充质干细胞样本。

The Tg for a mass ratio mixture of Galpi and sucrose of 0.3/1 was greater than 0 and was similar to that of the same ratio mixture of raffinose to sucrose, indicating that Galpi as well as oligosaccharides plays an important role in glass formation.

甲基肌醇半乳吡喃糖和蔗糖以0.3对1重量浓度混合比例，其玻璃质化转移温度在0℃以上，且近似於相同比例之寡糖类和蔗糖混合，显示甲基肌醇半乳吡喃糖和寡糖类在玻璃质化形成上扮演重要的角色。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase，CTS1-2)是一种来自酿酒酵母的内切几丁质酶，它的酶切位点位于其同聚物内的β-1，4连接的糖苷键，酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质粒的启动子在糖多孢红霉菌中都不能发挥作用，故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子，并利用pSET152质粒上链霉菌染色体整合位点和氨朴霉素抗性基因，构建了表达载体pZMW。

Matrix extracelluar phosphoglycoprotein, which is one of the matrix extracelluar non-collage-nous proteins, is expressed in bone, teeth and renal proximal convoluted tubules.

细胞外基质磷酸糖蛋白是一种细胞外基质的非胶原性磷酸化糖蛋白，主要表达于骨组织、牙组织和肾近球小管中，在骨形成矿化以及调节磷吸收方面发挥重要作用。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新