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Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓,进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后,依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂(GM1)组、胶质源性神经营养因子组和GDNF+GM1组,以及对照组。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒 pHXB2中克隆了两段膜糖蛋白基因片段。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人溃疡免疫缺陷病毒发烧1(HIV-1)HXB2分离株原病毒发烧基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

The variations of ecological characteristics, soil salinity, phytochroms, polysaccharide, soluble sugars, pralines, malondialdehyde, membrane stability, inorganic cations, and antioxidant enzyme activities in Aloe vera L. irrigated with seawater having varying salinity were studied.

本论文以完全淡水浇灌的库拉索芦荟为对照,对库拉索芦荟在不同浓度海水浇灌下的生态特征和海水浇灌对土壤盐度的改善情况;以及库拉索芦荟叶中叶绿体色素、多糖、可溶性糖、脯氨酸、质膜透性、丙二醛、无机离子、抗氧化酶等几项生理指标的变化进行了研究。

The genome of wheat rosette stunt virus, a plant rhabdovirus, is a single negative strand RNA.

小麦丛矮病毒是在中国发现的一种植物弹状病毒,病毒基因组是由一条单链负链RNA组成并编码 5种病毒结构蛋白质:表面糖蛋白G、膜基质蛋白M、核衣壳蛋白N、大蛋白L和所谓非结构蛋白NS。

It is possible to preparing neutralizing MAb which target to active region of Stx2 by synthetical B cell epitope peptides. At present, the crystal structure of the compound of Stx2 and its ligand adenines has been resolved, and the active site of Stx2 is definite, which is the seventy-seventh amino acids, Ganimalon, of Stx2 A1 fragment.

Stx2通过B亚基与受体Gb3结合后,进入细胞,在弗林蛋白酶(一种钙敏感的丝氨酸蛋白酶)作用下A亚基被裂解为A1和A2两个片段,A1片断经内质网进入细胞质暴露毒性活性位点―A1片段发挥N-糖苷酶活性,导致真核细胞蛋白质合成的终止,最终导致细胞的死亡。

Cellular adhesion molecule is a kind of glucoprotein expressed on the membrane surface of various cells,which mediate the conjunction and adhesion of cells and extracellular matrix.Vascular cellular adhesion molecule-1(VCAM-1) and intercellular adhesion molecule-1 (1CAM-1) are members of CAM.

细胞粘附分子(cellular adhesion molecule,CAM)是一类由多种细胞产生的,调节细胞和细胞间以及细胞和基质间相互结合并起粘附作用的膜表面糖蛋白,主要包括血管细胞粘附分子-1(vascular cellular adhesion molecule-1,VCAM-1)和细胞间粘附分子-1(intercellular adhesion molecule-1,ICAM-1)等。

As support, Agarose can benot only used in normal condition, but also in denaturalization (for example, 6 mol/Lhydrochloric acid carbamidine or 8 mol/L urea). Herein, the preparation of IMACmatrixes were conducted using agarose as support, carboxymethylated aspartate as chelating ligand and Co~(2+) and Ni~(2+) as chelating center ions.

本实验用琼脂糖作为基质,选用四配位,与金属离子有较强作用力的羧甲基天冬氨酸为配基,以Co~(2+)、Ni~(2+)作为螯合金属离子,合成了一系列金属螯合亲和色谱填料,并对其蛋白质分离性能进行了研究。

For instance Bavarian cream should not deviate from the original recipe but can be stabi li zed and preserved with the addition of more gelatine and sugar; possibly the dessert can be polished with a thin coat of wine jelly or something similar.

举例来说巴伐利亚奶油不应该与最原本的配方有太大的不同,但是可用较多的胶质和糖来保存和增加持久性。可能地话,餐后甜点上也要加上一层类似葡萄酒冻或相似的东西以增加亮度。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。