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During strawberry ( Fragaria ananassa Duch cv.chunxing) fruits maturation,the content of total sugar,anthocyanin and respiration rate increased significantly,and Ca 2+ _ATPase activity of cytoplasm and microsome membrane increased firstly,then peaked at pink stage,and decreased at full red stage in fruits,and also,the activity of plasma membrane Ca 2+ _ATPase was the highest in microsome membranes.

'春星'草莓果实成熟时,总糖和花青苷含量增加,呼吸速率也显著升高;同时,细胞溶质Ca2 + _ATPase活性和微粒体膜的Ca2 + _ATPase总活性变化具有相似的特点,即先升高,至粉红期达到高峰,全红期又下降,在微粒体膜中以质膜Ca2 + _ATPase占的比例最高。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定,得到了5个质谱阳性鉴定结果,其中2个蛋白分别为玉米种属的1,5-二磷酸核酮糖羧化酶/加氧酶和叶绿体的核酸结合蛋白;其它的3个蛋白在玉米蛋白质数据库中未见报道,但与其它物种基因编码的蛋白质具有高度的序列同源性。

Methods:Six surfactants-bean phospholipid,pulmonary surfactant,rhamnose,imidazoline,natrium glycocholic acid and natrium glyceric acid were chosen as accelerator to made into insulin solutions,and then use tween-80 and span-80 as matrix to made insulin aerosols,which were ultrasounicly nebulized and inhaled by SD rats through nose.

选用豆膦脂、肺表面活性剂、鼠李糖、咪唑啉、甘氨胆酸钠和甘珀酸钠六种表面活性剂为促进剂,制成胰岛素溶液,再以吐温-80、司盘-80做基质制成胰岛素气雾剂,经超声雾化,自SD大鼠鼻腔吸入,分别检测其血糖降低程度。

BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞,对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养,实验组用条件培养液(基础培养液,10 mmol/Lβ-甘油磷酸纳,10-7 mol/L地塞米松,50 mg/L维生素C)进行诱导培养。

Methods: turkish gall 1,turkish gall 2 were selected to experiment medicine and there medicines to streptococcus mutans atcc25175, actinomyces naeslundiiwvu627, actinomyces viscosus atcc19246 and lactobacillus rhamnosus ac413 were checked, to observed the change of glycose metabolism and acid metabolism.

选择4种与龋病密切相关的口腔细菌作为实验菌株,以没食子鞣质及其提取物2为实验药物,研究对口腔细菌产酸的影响;同时选择主要产糖菌变形链球菌和粘性放线菌为实验菌株,研究实验药物对其合成水不溶性胞外多糖能力的影响。

The results show that the hyperthyroidism group has elevated levels of choline, glucose and declined levels of VLDL, LDL, cholesterol, lactate, glycoprotein and alanine in serum samples relative to the healthy group. In urine samples, the hyperthyroidism group show increased levels of glucose, citrate, taurine and creatinine as well as decreased levels of hippurate, trimethylamine-N-oxide, formate and succinate.

结果表明,甲亢组血清中的胆碱、葡萄糖和三甲胺等物质的含量升高,而VLDL,LDL和胆固醇等脂质以及乳酸、糖蛋白和丙氨酸等代谢物的含量下降;甲亢组尿液中的葡萄糖、柠檬酸、牛磺酸以及肌氨酸等代谢物的含量升高,而马尿酸、TMAO、甲酸和琥珀酸等代谢物的含量下降。

The dopamine release in vivo was monitored by fast cyclic voltammetry and the concentrations of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic acid were determined by high performance liquid chromatography electrochemical detection in the process study. In addition, the effects of some traditional Chinese herbs and related marine drug on behavioral changes were also evaluated in order to provide some suggestive clue for rational drug design of anti-Parkinon's disease .

许多因素都参与PD的DA能神经元的死亡,所以,本研究在制备PD动物模型的基础上,应用国内率先引进的快速周期伏安法检测设备在体动态监测DA的释放,同时应用另一种电化学检测技术—高效液相色谱电化学检测法离体检测DA及其代谢产物二羟苯乙酸和高香草酸的含量,结合行为学测定及其它方法,研究了雌激素和铁在PD发病中的作用,以及一些药物,如吲哚醌、牛磺酸、铁离子螯合剂、胆囊收缩素(CCK-8)、乌鸡白凤丸有效成份、大豆异黄酮及海洋酸性寡糖HSH971,对DA能神经元的影响,从DA能神经递质角度对PD的发病机理提供进一步的实验依据,并为PD的预防和治疗提供新的干预措施。

BACKGROUND: Lipopexia induced by glucocorticoid is fat precipitation in marrow due to abnormal lipometabolism, or differentiation of cells resulting from hormone-affected bone marrow mesenchymal cells.

摘要背景:糖皮质激素引起的髓内脂肪蓄积是由机体脂肪代谢异常在髓内形成的脂肪沉淀,还是由于激素直接作用于骨髓间充质细胞使细胞发生了分化,以及具体生成的过程目前还没有定论。

BACKGROUND: Lipopexia induced by glucocorticoid is fat precipitation in marrow due to abnormal lipometabolism, or differentiation of cells resulting from hormone-affected bone marrow mesenchymal cells. The precise generating procedure remains unclear. OBJECTIVE: To investigate effects of various concentrations of dexamethasone on adipogenic differentiation of bone marrow mesenchymal cells.

背景:糖皮质激素引起的髓内脂肪蓄积是由机体脂肪代谢异常在髓内形成的脂肪沉淀,还是由于激素直接作用于骨髓间充质细胞使细胞发生了分化,以及具体生成的过程目前还没有定论。

Methods Based on molecular assembly technique, the matrix of agarose gel was deposited on the surface of glass plates,followed by drying, oxidation with NaIO 4 and cross linking with glutaraldehyde.

利用分子组装技术,以琼脂糖凝胶为基质,于玻片表面制成自组装膜,经过碘酸钠氧化后再与戊二醛分子偶联,而成为具有双功能分子层的薄膜。

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