糖质

Negative Fast Atom Bombardment and Collisional Activation Mass Spectrometry in the Structural Analysis of Oligoglycosides

负离子快原子轰击及其碰撞活化质谱法在寡糖苷结构分析中的应用

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

With aggravation of drought stress, ZXY04P-75 and ZXY04P-201 increased in proline content while that of ZXY03P-173 and ZXY04P-239 increased initially, reaching the peak at the 8th day, then declined. And the rate of net photosynthetic declined notably with the intensity of drought stress, The minimum of decline rate of net photosynthetic is ZXY04P-201. 4. The results of photosynthetic mechanism for 4 Dactylis glomerata L. show: the net photosynthetic rate, transpiration rate,stomatal conductance and chlorophyll decreased dramatically, Compared with weak drought resistance ZXY04P-239,the net photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll in the leaves strong drought-resistance ZXY04P-201 decreased slowly.

连续水分胁迫及复水条件下4份鸭茅抗旱机理的研究表明，叶片水分饱和亏缺随着干旱胁迫强度的增加而增加，其中变化幅度最大的是ZXY04P-239，最小的是ZXY04P-201；ZXY03P-173和ZXY04-201质膜相对透性和可溶性糖含量随着干旱胁迫强度的增加而增加，而ZXY04P-75和ZXY04P-239出现单峰变化，在胁迫第8d后达到最大，然后下降；ZXY03P-173和ZXY04P-239脯氨酸含量在干旱胁迫第8d达到最大，而ZXY04P-75和ZXY04P-201的脯氨酸含量随着干旱胁迫程度的加深而逐渐达到最大；4份材料叶片的净光合速率随着干旱胁迫强度的增加而减小，其中ZXY04P-201的净光合速率下降率胁迫至12d时为最小。

The comprehensive evalucation resulted in a strong drought resistance group including ZXY04P-201、ZXY03P-227、ZXY03P-122 and ZX Y04P-136, a weak drought resistance group including ZXY04P-101、ZXY04P-89,ZXY04P-239 and a intermediate group including the others according to the the above indicators. 3. The results of study on drought mechanism for 4 Dactylis glomerata L. under continuously water stress and rewatering show: the water saturation deficit of leaves arose hastily, and ZXY04P-239 changed most obviously while ZXY04P-201 changed only slightly under drought stress. ZXY03P-173 and ZXY04P-201 increased in the relative plasmalemma permeability and the soluble sugar content while thant of ZXY04P-75 and ZXY04P-239 showed the trend of initial increasing, reaching the peak at the 8th day, then decreasing under drought stress.

20份鸭茅种质幼苗期抗旱性研究表明，干旱胁迫条件下，所有材料的株高、生物量显著下降，叶片脯氨酸和可溶性糖作为重要的渗透调节物质，其含量迅速增加，质膜相对透性的数值变大，细胞膜受伤害程度增大，水分饱和亏缺值变大，植物保水能力变弱，胞间CO2浓度增大；净光合速率、蒸腾速率、气孔导度和叶绿素含量等指标测定值减小，通过20份鸭茅种质苗期抗旱性综合评价，发现ZXY04P-201、ZXY03P-227、ZXY03P-122、ZXY04P-136属强抗旱性材料，ZXY04P-101、ZXY04P-89、ZXY04P-239属弱抗旱性材料，其他属于中等抗旱性材料。

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无泪配方，无麻醉成分对乳痂头皮有清洁和舒缓作用，促进受刺激头皮愈合对油性肤质是一款不错的清洁用品我们的香薰精油都经过纯度测试，保证不含任何杀虫剂成分使用本品后，头发清爽富有光泽且不会损伤娇嫩的头皮粘膜。抗真菌/普通细菌我们的采用的清洁成分—癸基聚葡糖，温和无刺激性，完全可以生物降解安全，温和，有效不含十二烷基硫酸钠或二乙醇胺本产品性质温和，在初生宝宝第一次洗澡时，妈妈们足以放心使用。

Ten transformants and 10 conjugons showed high-level resistance against aminoglycoside antibiotics,all of which the value of MIC＞256μg/mL carried armA gene.Conclusions:The Ab clinical isolates from burn ward in Gansu Province People\'s hospital have high drug-resistance.16S rRNA methylases gene exsist in Ab and locate in plasmid chromosome.They are easily disseminative,which demonstrated a very high level of resistance to various aminoglycosides.

甘肃省人民医院烧伤病房鲍氏不动杆菌临床分离株有非常高的耐药率，16S rRNA甲基化酶基因armA在鲍氏不动杆菌中存在，且位于质粒染色体上，易于传播和扩散，导致所有氨基糖苷类抗菌药物高水平耐药。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带，用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

Existing plasmids and inherited materials are used to construct expression vector pZMW which could be integrated into S. erythraea chromosome and multi-copy expression vector pZM.

本实验室利用现有的质粒和遗传材料，构建了糖多孢红霉菌染色体整合型表达载体pZMW和游离型多拷贝表达载体pZM。

Lactferrin,primely used as anti bacterial protein,is a kind of ferrum binding glycoprotein.

Lactferrin是一种铁结合性糖蛋白质，最初作为抗菌蛋白质被广泛研究。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后，加入不同浓度的碘化钾，观察细胞的形态改变；琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解；MDA法测定细胞膜的脂质过氧化程度。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。