糖苷配基

In the case of glycoside, the aglycome may be synthesized by one or a combination of the pathways

在糖苷的情况下，糖苷的配基可以通过一个或几个途径合成。

In the case of glycoside, the aglycome may be synthesized by one or a comb in ation of the pathways

在糖苷的情况下，糖苷的配基可以通过一个或几个途径合成。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The chapter 6 makes a study on the design and synthesis of new glycosides, which prepared from imidazole, thiazole and tetrazole aglycones mentioned above and glucose derivatives by phase transfer catalysis under the moderate reaction condition.

我们以合成的新杂环化合物作为糖的配基，用相转移催化法合成了25个糖苷类化合物。其中包括4个咪唑氮糖苷，12个含有噻唑环的糖苷，6个含有噻唑环的糖酯，3个四氮唑糖苷。

The correlations of eleven floral and fruit quantitative traits of Liriodendron interspecific hybrids(L. chinense×L. tulipifera) and its parent species were analysized.The aglycone and glycoside of flavonoid and carotinoids of perianth segment in the three groups were studied qualitatively and quantatively by high performance liquid chromatography.The results were as followed: The aglycone such as cyanidin, 6-hydroxyflavone and trans-chalcone and the glycoside such as glucose, rhamnose and galactose were discovered in Liriodendron interspecific hybrids and Liriodendron chinense,carotinoids such as lycopene and β-carotene were also discovered.

本文对鹅掌楸、北美鹅掌楸和杂交F_1的花果共11 个数量性状进行分析，并应用高效液相色谱对上述三大类群花被片类黄酮糖苷配基和结合糖及类胡萝卜素进行了定性和定量研究，结果如下：鹅掌楸和杂交鹅掌楸花被片类黄酮糖苷配基为花青素、6-羟基黄酮和反式查尔酮，糖苷配基结合糖为葡萄糖、鼠李糖和半乳糖；类胡萝卜素为β-胡萝卜素和番茄红素。

The binding behavior and kinetics of uncoupling protein with its ligand, the Fourier-transformation infrared spectra of uncoupling protein and its changes during ligand-binding process, the inhibition of Guanosine 5＇-Tetraphosphate (G-4-P) and Adenine-β-Darabinofuranoside 5＇-triphosphate on the binding of uncoupling protein with GTP were studied.

本文对解偶联蛋白与配基的结合及其结合动力学，解偶联蛋白及其与配基结合过程中的傅里叶变换红外光谱及其光谱变化，鸟嘌呤核苷四磷酸（G-4-P）和9-β-D-阿拉伯糖基呋喃5＇-三磷酸对解偶联蛋白与GTP结合的影响进行了研究。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。