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Trehalose is a disaccharide consisted of two subunits of glucose withα-1,1 glucosidc bond .It has biology functions of unique anti-frozen and anti-dehydration.

海藻糖是由2分子的葡萄糖经α-1,1糖苷键结合而成的非还原性二糖,具有独特的抗冷冻和抗脱水的生物学功能。

Selectivity modification of glycoside by glycosidase is one of the most fascinating topics.

利用糖苷酶对糖基化合物的糖基定向改造工程是目前一个研究的热点。

By orthogonal test,the optimum conditions for soybean isoflavone glycoside to be hydrolyzed into isoflavone aglycon were found.

通过正交试验得到了大豆异黄酮糖苷水解为大豆异黄酮苷元的最佳工艺条件。

Mucopolysaccharide was a kind of natural molecular, which was basically consisted of aldoses linked by indicant bond, and was almost found in all organisms including animal, plant and microbe.

粘多糖是由糖苷键连接起来的醛糖或酮糖组成的天然分子,几乎存在于所有的有机体中,包括动物、植物、微生物。

The radiated samples of yeast mannan and their control were measured by FT-IR, Vis-UV and scanning electron microscope, then they were observed and analyzed, and compared with the above two data.

它们既是糖分子内、分子间形成氢键和糖苷键的结构基础部件,又是甘露聚糖糖基化其他生物大分子进而影响它们在生物信息流中作用的重要结构部件。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The described procedures provide an efficient method to synthesize bicyclic azasugar and thiosugar with novel scaffold as potential glycosidase inhibitors.

本方法提供了一种合成具有潜在糖苷酶抑制活性的氧桥双环氮杂糖和硫杂糖的有效途径。

The position , type and number of sugar moieties incorporated to the antibiotics have great impact on its bioactivity .

2 糖苷类抗生素中糖基的主要作用糖基化的作用和意义主要体现在以下三个方面:第一,增加化合物的水溶性。

This work made preparations for the further in-vitro study on the activity of glycosyltransferase and the usage of different glucosyl.Several important factors for protoplast preparation from Amycolatopsis-orientalis were studied and optimized such as the type of substrate, the concentrations of the addition of glycin, the concentrations of lysozyme and so on.

并且通过酸水解的方法获得了脱去一个糖基的万古霉素假糖苷,可以将其作为酶转化反应的底物,为进一步在宿主菌体外研究糖基转移酶的活性以及不同糖基的生理作用做好准备。

And physicochemical procedures (molecular weight measurement, viscosity analysis, etc.). All glycans (polysaccharides and peptidoglycan) were found as glucans, but each glucan has its specific sugar chain structure, including glycosyl reside linkages and sugar chain conformation.

结构研究表明,除PSG6为肽多糖以外,其他六个多糖均为由单一葡萄糖构成的葡聚糖,但各多糖在糖残基连接方式,糖苷键构型,支链的长度、取代度与在主链上的分布及糖链构象上有显著差异。

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