糖苷的

To enhance the efficiency of cellulose bio-degradation and reduce the mass transfer resistance from cellulose consequently increase the extraction rate of diosgenin, glucose was separated andβ-glycosidase was added in to decompose cellobiose in the biocatalysis pretreatment.

为了提高纤维素酶的作用效率，降低薯蓣皂苷元提取过程中纤维素带来的传质阻力，从而提高薯蓣皂苷元产率，在酶解的预处理过程中对糖液进行分离并添加β-葡萄糖苷酶分解体系中的纤维二糖。

These two isolates presented typical characteristics of Streptococcus iniae with gram-positive cocci in chains, catalase negative and beta-hemolytic, growth under 10℃ and non-growth up 45 ℃, hydrolyzation of esculin and arginine, Voges-Proskauer, urease, and hippurate tests negative, fermentation of glucose, salicin, sucrose and starch, non-fermentation of arabinose, inulin, lactose, melibiose, raffinose and sorbitol.

实验结果表明，两菌株具有典型的海豚链球菌特性：在显微镜下为球形细胞，呈长短不一的链状排列，革兰氏染色阳性：在血琼脂平板上显示β溶血；1O℃生长，45℃不生长；接触酶阴性；水解七叶苷、精氨酸，VP试验、脲酶和马脲酸试验阴性；发酵葡萄糖、水杨苷、蔗糖和淀粉，不发酵阿拉伯糖、菊糖、乳糖、蜜二糖、棉子糖和山梨醇等。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Based on the above-mentioned, two kinds of target glycoclusters with different glycoterminus, flexible linkers and different scaffolds were designed, disaccharide glycocluster (including TM1-TM7) and trisaccharide glycocluster (TM8-TM9) respectively, with the purpose of obtaining glycoclusters with high binding affinity to anti-Gal antibody and thus better inhibiting the HAR.

基于上述，针对HAR产生的机制，以人体中天然存在的anti-α-Gal抗体及B细胞表面的相应受体为靶点，以α-Gal抗原末端的二糖Galα1→3Gal和三糖Galα1→3Galβ1-4GluNAc片段为糖基部分，采用柔性的连接臂（氨基保护的2-乙醇胺和3-巯基丙酸）和刚性的芳香骨架及柔性的脂肪链骨架，设计了两类结构新颖的糖簇分子：即氧苷和硫苷键连接的二糖糖簇TM1-TM7）和三糖糖簇（TM8-TM9），目的在于寻找与anti-Gal抗体及B细胞相应受体有较高结合力的糖簇分子，以便有效地抑制HAR反应的发生。

Sixteen compounds were separated from 80% ethanol extract of Plant A, they were:-lariciresinol,-isolariciresinol,-isolariciresinol 9-O-β-D-glucopyranoside, tanegoside B, apiginin, apigenin 7-O-β-D-glucoside, apigenin 7-O-β-neospheroside, quercetin 3-O-β-D-glucopyranoside, 1, 7-dihydroxy-3, 4-dimethoxyxanthone, 3-O- [α-L-rhamnopyranosyl (1-4 ] -β-D-glucopyranoside-(25S)-5β-spirostan-3β-ol , 3β-O-D-glucopyranoside quinovic acid, mangiferin , 3'-p-hydroxybenzoyl-mangiferin, physcion, benzenyl methanol β-D-glucopyranoside and Vanillic acid.

结果： 1。从植物A的80％乙醇提取物中共分离得到16个化合物，分别为：-落叶松脂醇、-异落叶松脂醇、异落叶松脂醇9-O-β-D-吡喃葡萄糖苷、tanegoside B、芹菜素、芹菜素7-O-β-葡萄糖苷、芹菜素7-O-β-新橙皮糖苷、槲皮素3-O-β-D-吡喃葡萄糖苷、1，7-二羟基-3，4-二甲氧基-口山酮、菝葜皂苷元-3-O-[α-L-吡喃鼠李糖基（1-4）]-β-D-吡喃葡萄糖苷、喹诺酸3β-O-D-葡萄糖苷、知母宁、3′-对羟基苯甲酸-知母宁、大黄素甲醚、苯甲醇-β-D-吡喃葡萄糖苷、香草酸。

Galactosidase can hydrolysis a -galactosis bond , namely it can hydrolysis galactosis complex of non-reductive ends binding α-bond, so it can hydrolysis oligosaccharides such as raffinose、 stachyose、 verbascose, et al. It also can hydrolysis heteropolysaccharide including α-galactosis bonds.

能催化α-半乳糖苷键的水解，即能水解非还原性末端以α键结合的半乳糖苷化合物，因此它能水解蜜二糖、棉子糖、水苏糖和毛蕊花糖等低聚糖，还能水解含有α-半乳糖苷键的杂多糖。

Methods:Alpha-glucosidase activities were examined with or without Folium Mori alkaloids extract,Ramulus Mori extract and acarbosein vitro, and the IC_(50)were calculated.

本文以拜糖平为阳性对照，采用α-葡萄糖苷酶体外抑制作用实验，比较了桑叶生物碱浸膏、市售的桑枝提取物以及拜糖平对α-葡萄糖苷酶的抑制作用能力差异。

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

In plant,the enzymes of RFO,such as galactinol synthase,myo inositol-1-phosphate synthase, raffinose synthase and stachyose synthase,are linked with the RFO pathw.

寡糖代谢是一个复杂的调控体系，其中肌醇-1-磷酸合成酶、肌醇半乳糖苷合成酶、蔗糖合成酶、棉子糖合成酶、水苏糖合成酶和毛蕊花糖合成酶等参与了棉子糖系列寡糖的生物合成过程。

The galactosyl oligosaccharides of raffinose series are widely distributed in seeds of many species and are localized in tissues that remain viable after desiccation, including the embryo and aleurone layer of cereals, cotyledons and axis tissues of legumes and other dicots.

棉子糖半乳糖苷系列寡糖广泛分布在许多种植物种子中，并存在于干燥后仍能保持活力的组织内，如禾谷类种子的胚及糊粉层，豆类及其他双子叶植物的子叶和胚轴组织等。棉子糖半乳糖苷系列寡糖在禾谷类种子的非自溶性中央胚乳中不合成，但存在于蓖麻种子的自溶性胚乳细胞中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。