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We investigated constituent of triterpenoid saponins ofAlbizzia, two new compounds together with two known compounds were isolated from Albizzia julibrissin Durazz. by using column chromatography (macroreticular resin, silica gel, Sephadex gel, reverse phase silica gel),preparative HPLC methods et al.On the basis of spectroscopic analysis, including IR,ESI-MS,~1H-NMR,~(13)C-NMR,HMBC,HMQC,~1H-~1HCOSY and chemical methods, the structure of two new compounds were identified as 3 - O -[β-D-xylopyranosyl(1→2)-β-D-fucopyranosyl (1→6)-β- D -2- deoxy - 2 - acetoamidoglucopyranosyl] -21-O-[(6S)-2- trans- 2,6-dimethyl - 6 - O-β- D - quinovopyranosyl -2,7- octadienoyl] - acacic acid- 28 - O-β-D-glucopyranosyl(1→3)[α-L-arabinofuranosyl(1→4)]-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside acacic acid 3- O -β- D- glucopyranosy(1→3)-β- D- fucopyranosl(1→6) [β-D- xylopyranosyl (1→2)]-β-D-glucopyranoside ;two known compounds were acacic acid lactone 3- O -β-D- xylopyranosyl-(1→2)-β-D-fucopyranosl (1→6)- 2-deoxy -2 -acetoamido -β-D- glucopyranoside ; acacic acid lactone 3- O-β-D-xylopyranosyl(1→2)-α-L- arabinopyranosl (1→6)- 2- deoxy - 2- acetoamido -β-D-glucopyranoside . The study lays chemical foundation and chemical reference substance for enhancing quality standard of Albizzia julibrissin Durazz.

本研究论文在综述国内外对合欢属Albizzia三萜皂苷化学成分和药理作用研究进展的基础上,利用传统植化分离手段和现代分离技术,包括大孔树脂、硅胶、葡聚糖凝胶、反相硅胶等柱色谱,制备高效液相色谱法等技术从中药合欢皮中分离得到了4个化合物,其中,2个新化合物和2个己知化合物,并进一步通过现代分析技术IR,ESI-MS,~1H-NMR,~(13C-NMR,HMBC,HMQC,~1H-~1HCOSY等和化学方法鉴定了2个新化合物的结构分别是:3-O-[β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖基]-21-O-[(6S)-2-反式-2,6-二甲基-6-O-β-D-吡喃鸡纳糖基-2,7-辛二烯酸基]-金合欢酸-28-O-α-L-呋喃阿拉伯糖基(1→4)[β-D-吡喃葡萄糖基(1→3)]-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷,金合欢酸3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃夫糖基(1→6)[β-D-吡喃木糖基(1→2)]-β-D-吡喃葡萄糖苷;2个已知化合物结构分别是:金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷,金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-α-L-吡喃阿拉伯糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因;一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因;片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源,但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性;片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源,而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性;另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase,CTS1-2)是一种来自酿酒酵母的内切几丁质酶,它的酶切位点位于其同聚物内的β-1,4连接的糖苷键,酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

The 1:1 mixtures of chaconine and solanine or solamargine and solasonine, produced markedly synergistic effects while the mixtures of unpaired glycoalkaloids from different plants did not have this effects.

卡茄碱与茄碱,澳洲茄边碱与澳洲茄碱的1:1的混合物产生了明显的协同作用,而来自于不同植物的不成对的糖苷生物碱则没有这种协同作用。

Results These 48 Staphylococcus epidermidis isolates had different resistance to aminoglycusides. The resistance and susceptibility to gentamicin was 69% and 31% respectively. The resistance, susceptibility and intermediary to netilmicin was 8%, 79% and 13%, streptomycin was 42%, 35% and 23% and amikacin was 15%, 75% and 10%.

结果 48株临床分离的表皮葡萄球菌对4种氨基糖苷类抗菌药物的药敏特征分别为:对庆大霉素的耐药率为69%,敏感率为31%;对奈替米星的耐药率为8%,敏感率为79%,中介率为13%;对链霉素的耐药率为42%,敏感率为35%,中介率为23%;对阿米卡星的耐药率为15%,敏感率为75%,中介率为10%。

Changes of leaf pigment content,soluble sugar content,enzyme activity and photosynthesis characters in leaf of Photinia×frasery"Red Robin"were searched of five different shading treatments(100%,80%,50%,25%,10%transmission rate).The seasonal variation of leaf color and stability of anthocyanin were studied in this paper.Purpose of the research is to master the Physiological characters during leaf color transformation of Photinia×frasery"Red Robin",and provide evidences to promote introduction cultivation and the application under different garden environment of them.

本文以2年生红叶石楠(Photinia×frasery'RedRobin)扦插苗为试材,探讨了不同遮光处理透光率分别为100%(全光照、80%、50%、25%、10%条件下叶片色素含量、可溶性糖含量、苯丙氨酸解氨酶活性、抗氧化酶活性变化及光合作用日变化,同时对其叶色的季节变化及花色素苷的稳定性进行了初步探讨,以掌握红叶石楠叶色变化的生理特征,为红叶石楠的广泛引种栽培及在不同园林环境中的应用提供科学依据。

The results showed that Kaidimycin was chemically neutral, macrocylic polylactones, stable and liable to dissolve into water and pyridine, slight to dissolve into methanol, ethanol and N-butanol, difficult to dissolve into ethyl acetate, acetone, benzene and chloroform. Chemical reaction of functional group of Kaidimycin showed that it had glycoside, amino acid and aldehyde, no reducing sugar, polypeptide and phenol.

凯地菌素属中等极性的、中性抗生素,极性纸层析结果与多烯类抗生素的纸层析结果相似:易溶于水、吡啶,微溶于甲醇、乙醇、正丁醇,不溶于乙酸乙酯、丙酮、苯、三氯化碳;对高温、光照和紫外处理有很好的稳定性;官能团反应试验结果发现,凯地菌素可能含有糖苷键、氨基酸伯氨基和醛基,不含有还原糖、多肽和酚基,高锰酸钾、浓硫酸反应与多烯大环内酯类抗生素的特性相似;并且依据纯品,采用生物法和分光光度法对摇床发酵液进行了效价的测定。

ARA has performed both wind-tunnel tests and CFD computations for numerous Customer projects over the past 30 years. One such example is the investigation the interference effect of various boom support locations on the flow over a civil aircraft empennage; the accompanying figure shows the flow field in the region of the empennage, as computed by an Euler flow solver.

阿糖胞苷的表现都风洞试验和计算流体力学计算为众多客户项目在过去30年里,其中一个例子是,调查干扰的影响,各种蓬勃支持的地点,对流动的一个民用飞机尾翼;随行的数字显示流量外地在该地区的尾翼,作为计算一欧拉流求解。

Cyclodextrins, cyclic oligosaccharides commonly composed of six, seven or eight respectively D-glucopyranosyl units connected by α-(1, 4)-glycosidic linkages, have the ability to form inclusion complexes with a wide range of substrates for their particular structures. With proper rigidity and flexibility, excellent mechanical stability and biocompatibility, cyclodextrins are good choice of material in molecular imprinting technology.

环糊精是由六,七或八个D-吡喃葡萄糖单元通过α-1,4 糖苷键连接成的环状低聚糖,由于其特殊的结构使其可以包结一系列的底物,加之具有适当的刚性、柔性、机械稳定性,良好的热稳定性和生物相容性等使其成为分子印迹技术优秀的选择材料。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现,十二烷基聚葡糖苷C_(12G_(1.50)/正丁醇/环己烷/H_2O体系形成的油包水微乳液中,脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应,反应初始速率V_0随正己酸的初始浓度C_~0增大而增大;随正丁醇的初始浓度C_~0的增大而减小,后V_0进入一&坪区&。

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