糖脂

Aryl tetralin of lignans in podophyllotoxin has significant anti-tumor activity, which podophyllotoxin glucoside derivatives etoposide (VP-16) and teniposide (VM-26) is widely used in clinical.

芳基四氢萘类木脂素中的鬼臼毒素具有显著的抗肿瘤活性，其中鬼臼毒素的糖苷衍生物etoposide(VP-16)和teniposide (VM-26)被广泛用于临床。

Objective: To probe the effects of trigonella foenum graecum saponin on blood fat, liver function, kidney function of the blood fat disorder model of Male Wistar rats.

采用高糖高脂饲料喂养Wistar大鼠2个月后，诱导出大鼠血脂紊乱模型。

Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

Objective To discuss the assay methods and clinical value of IgG antibody to Mycobacterium Tuberculosis. Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

目的 探讨结核分枝杆菌IgG抗体的检测及其临床应用价值方法应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

After dipyridamole was bound to the cell membrane, the test sample was obtained by ultrasonically dissolving the labeled membrane in 10mmol L〓 tris-HCl membrane lyses solution.

氨基酸、糖、药物、肽、核苷、离子和神经递质等物质不易通过细胞膜的脂质双层，而必须通过细胞膜上的载体蛋白转运体协助才能进入细胞。

Aim To develop a sandwich ELISA for measuring serum β2-glycoprotein I-lipoprotein complex (β2-GPI-Lp concentration, and to explore the existence of serum complexes β2-GPI-Lp and its clinical value for IgA nephropathy. Methods ELISA was established using rabbit anti-human β2-GPI antibody as the capture antibody, and quantitating with polyclonal antibody against apolipoprotein enzyme conjugate.

目的 建立检测人血清β2-糖蛋白I-脂蛋白复合物的酶联免疫吸附法，探讨该复合物存在于人血循环中的可能性，并通过对IgA肾病患者的检测分析该复合物的临床诊断价值。

Cocoa powder could now be combined with sugar and remixed with cocoa butter to create solid chocolate.

剩下干的&蛋糕&加工成可可粉，可可粉现在可以和糖混合同时和可可脂混合加工成固体的巧克力。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Which can be used as the body of vitamin B1 coenzyme of many enzymes in the normal human body has an important role in glucose metabolism, it can inhibit the activity of cholinesterase, and choline esterase is an important neurotransmitter, and thus nerve conduction impact; vitamin B2 in the organization by participating in a variety of yellow enzymes constitute a coenzyme, its role in bio-oxidation process in the delivery of hydrogen role in regulating the body's metabolism process; vitamin B6 can also be a variety of enzymes as coenzyme participate in all kinds of amino acid metabolism, promote the absorption of amino acids and protein synthesis, cell growth is necessary for the material, which also promote fat metabolism, involved in neurotransmitter synthesis, hemoglobin synthesis and the impact on the normal reproductive functions such as human body an important metabolic process, Volkswagen Medicine Net: What liver protection effect of vitamin B family?

可作为维生素乙许多酶的辅酶，在正常人体组织中有一个用糖代谢的重要作用，能抑制胆碱酯酶活性，和胆碱脂酶是一个重要的神经递质，神经传导，从而影响；由黄色参与各种酶在组织中维生素B2构成辅酶，其作用在生物氧化氢的作用，调节人体的代谢过程中产程；维生素B6也可以是作为辅酶参与氨基酸代谢的各种酶的品种，促进氨基酸的吸收和蛋白质合成，细胞生长是必要的材料，这也促进脂肪代谢的神经递质合成，血红蛋白合成和参与，如影响人体生殖功能的正常新陈代谢过程中一个重要的，（大众医药网：什么样的维生素B家庭肝脏的保护作用？

Kotina is a Refined ,Bleached and Deodorised Fractionated Palm Kernel Oil based HARD BUTTER with excellent keeping qualitiesAPPLICATIONSKoatina is Bland and Odourless with excellent palatabilityKoatina can also be used for coating and center application in Wafers, Crakers, Nuts and Caramels.

Kotina 是提炼，被漂白的和除臭的被分馏的棕榈橄榄油基于的坚硬黄油与优秀保持的qualitiesAPPLICATIONSKoatina 是平淡和无气味的以优秀palatability Koatina 可能被使用为饼干提取乳脂和奶糖装填 Koatina 可能并且被使用为涂层和中心应用在薄酥饼、Crakers 、坚果和焦糖里。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。