糖胶

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白，再经割胶回收法得到进一步纯化的重链片段，分子量约为75kDa，免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清，为ELISA和Western blotting检测技术奠定了基础。

Water,butylene glycol,cyclomethicone,VP/VA copolymer,glyceryl stearate,glycerin,ppg-15 stearyl ether,titanium dioxide,propylene glycol,cetyl alcohol,citrus aurantium dulcisfruit extract,saccharide isomerate,biosaccharide gum-1,ethoxydiglycol,peg-40 stearate,glycyrrhiza glabraroot extract,morus bombycis root extract,potassium cetyl phosphate,phenoxyethanol,c12-20 acid peg-8 ester,triethanolamine,prunus amygdalus dulcis oil,carbomer,caprylyl glycol,caffeine,disodium edta,methylparaben,chondrus crispus,butylparaben,iris germanica root extract,pentadecalactone,sodium polyacrylate,ethylparaben,sodium hyaluronate,peg-8,propylparaben,citric acid,chlorhexidine digluconate,tocopherol,ascorbyl palmitate,ascorbic acid

水，丁烯二醇，环甲硅脂，VP/VA共聚物，甘油基硬脂酸，甘油，ppg-15 stearyl ether，二氧化钛，丙二醇，十六烷基醇，橙子萃取物，天然异构寡糖， biosaccharde gum-1，乙基氧基乙二醇，聚乙二醇- 40硬脂酸，甘草根提取物，桑白皮根萃取，钾十六烷基磷酸盐，苯氧乙醇，c12-20 acid peg-8 ester，三乙醇胺，甜杏仁油，高分子胶，辛乙二醇，咖啡因，乙二胺四乙酸二钠，对羟基苯甲酸甲酯，卡拉胶，对羟基苯甲酸丁酯，鸢尾花根萃取， pentadecalactone ，聚丙烯酸钠，羟苯乙酯，透明质酸钠，聚乙二醇- 8，羟苯丙酯，柠檬酸，洗必泰葡萄糖酸盐，维生素E，维生素C棕榈酸酯，抗坏血酸

Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen Ⅰ and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group, while there was no diference in ACE mRNA expression and activity between the two groups.

结果 APS组血糖、糖化血清蛋白、心肌酶谱和心肌Ang Ⅱ水平较DM组显著下降，胰岛素、C肽、血浆AngⅡ水平和DM组无差异；APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降；APS组chymase mRNA表达和活性均显著低于DM组，ACE基因的表达和活性与DM组无显著性差异。

In the present study,a novel microgel was designed by the incorporation of temperature sensititive N-isopropylacrylamide and pH sensitive acrylic acid to copolymerize with glucosamine derivative,acrylamido-2-deoxy-glucose. The novel microgel has favorable biocompatibility,tissue-targeting and stimuli-responsibility to pH and temperature.

本文利用具有生物相容性的氨基葡萄糖单糖衍生物、具有温敏性的异丙基丙烯酰胺及具有pH敏感性的丙烯酸共聚得到了全新的微凝胶，新型微凝胶具有良好的生物相容性、组织靶向性和对温度、pH的刺激响应性。

METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖，气相色谱法和薄层层析法测定其多糖组成，凝胶过滤法测定其相对分子质量，红外光谱测定其苷键类型，比色法测定其糖含量。

There is synergistic interaction maximum while the mixed ratio of KGM and Guran gum is 60/40,the total polysaccharide concentration 1%, TP 80℃ and salt ionic (Ca2+)concentration 0.1mol/L.Interraction machanism between molecules and molecules of two polysaccharide were investigated by FT-IR .

当总糖浓度为1%，魔芋胶与瓜尔豆胶的共混比例为60/40 ，制备温度为80℃，体系盐离子(Ca2+)浓度为0.1mol/L 时可得到协同相互作用的最大值，从FT-IR 谱图上分析了两种多糖分子间相互作用的机理。

Methods1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Using DNA-casting SDS-PAGE and agarose gel to monitor the activity of the nuclease in the process of extraction.

双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化以SDS-PAGE-DNA功能胶和琼脂糖凝胶电泳测活相结合作为双胸蚓组织核酸酶提取过程中核酸酶活性的监测体系。

Objective: Mannose-binding lectin is a plasma protein belonging to the family of collectins, composed of a N-terminal segment, helical coiled-coil hinge region, collagen-like stalk domain., and globular type C lectin domain or carbohydrate recognition domain. Full MBL biological function requires assembly to at least the tetrameric level.

目的：甘露聚糖结合凝集素(Mannose-binding lectin， MBL)是属于C型凝集素家庭胶原凝集素的一种血清蛋白，包括N末端区（N-terminal segment）、螺旋铰链区（helical coiled-coil hinge region）、胶原样区（collagen-like region，CRL）及球形C型凝集素区或糖识别区（carbohydrate recognition domain ，CRD）等4部分结构区域，四个亚单位的MBL形成有功能的多聚体。

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。