糖胶

Shengyuan maintenance blood-sugar capsule is chooses the cactus, the balsam pear, the black bee bee glue, the kudzu root four pelts the sugar ingredient reasonably to blend, purifies the highly effective multi-skill health product by the modern biotechnology extract which but becomes, has the bidirectional adjustment, the control blood sugar, the activation and the nutrition island of langerhans beta cell, the promotion endogenous secretions insulin secretion, enhances the human body sugar metabolism and the fat metabolism ability, strengthens the pancreas function the function, at the same time also has the repair diabetes liver, the kidney damage, the prevention and the improvement diabetes illness complication function.

圣源维糖软胶囊是选择仙人掌、苦瓜、黑蜂蜂胶、葛根四大降糖成份合理配伍，以现代生物科技萃取精制而成的高效复合型健康产品，具有双向调节、控制血糖，激活和营养胰岛β细胞，促进内源性胰岛素分泌，提高人体糖代谢和脂肪代谢能力，增强胰脏功能的作用，同时又具有修复糖尿病肝、肾损伤，预防和改善糖尿病并发症的作用。

Agarose ; basic resin ; gel strength ; gentian violet electrophoresis ; DNA gel electrophoresis

琼胶糖；强碱性树脂；凝胶强度；结晶紫电泳； DNA凝胶电泳

The protective effects of alginate oligosaccharides on the killing of Escherichia coll. by hypochlorous acid are examed.

结果表明：褐藻胶寡糖A1，A2和A3对3种自由基均有较好的清除作用，而且清除活性随糖浓度的增加而加强。

Water, Cyclomethicone, Glycerin, Sd Alcohol 40-B (Alcohol Denat.), Propylene Glycol, Melia Azadirachta Leaf Extract, Lecithin, Arctium Lappa Root Extract, Xylitol, Lactitol, Alpha-Glucan Oligosaccharide, Lactoferrin, Potassium Thiocyanate, Glucose Pentaacetate, Glucose Oxidase, Lactoperoxidase, Glycoproteins, Panax Ginseng Root Extract, Equisetum Arvense Extract, Hydrolyzed Milk Protein, Peg-8, Caprylyl Glycol, Sebacic Acid, Sodium Polyacrylate, Betula Alba Bark Extract, Cupressus Sempervirens Leaf Extract, Hamamelis Virginiana Extract, Avena Sativa Meal Extract, Humulus Lupulus Extract, Silk, Nylon-12, Polyacrylamide, Polymethyl Methacrylate, Butylene Glycol, Salicylic Acid, Lauryl Methacrylate/Glycol Dimethacrylate Copolymer, Tocopheryl Acetate, C13-14 Isoparaffin, Laureth-7, Triethanolamine, Xanthan Gum, Acrylates/C10-30 Alkyl Acrylate Crosspolymer, Phenoxyethanol, Chlorphenesin, Benzoic Acid, Methylparaben, Fragrance, Titanium Dioxide

水，环甲硅脂，甘油，Sd Alcohol 40-B，丙二醇，印度楝树叶萃取，卵燐脂，牛蒡根萃取，木糖醇，乳糖醇，α-葡低聚糖，乳铁蛋白，硫氰酸钾，葡萄糖五乙酸酯，葡萄糖氧化酶，乳过氧化物酶，糖蛋白，人参根萃取，问荆萃取，水解牛奶蛋白，聚乙二醇-8，辛乙二醇，癸二酸，聚丙烯酸酯钠，白桦萃取，丝柏叶萃取，金缕梅萃取，燕麦萃取，啤酒花萃取，丝，尼龙－12，聚丙烯醯胺，聚甲基丙烯酸甲酯，丁二醇，水杨酸，甲基丙烯酸十二酯，醋酸盐维他命E，C13-14 异烷烃，月桂醇聚醚-7，三乙醇胺，山羊胶，丙烯酸/C10-30烷基丙烯酸聚合物，苯氧乙醇，氯苯甘醚，苯甲酸，羟苯甲酯，香料，二氧化钛

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Spongy confection made of gelatin and sugar and corn syrup and dusted with powdered sugar.

像海绵的糖果，由凝胶、糖和玉米糖浆制成，然后撒上糖粉。

The pI of these components gradually decreased f rom pH 4.0 according to electrophoresis mobility from higher to lower on PAGE. The molecular weights were in the range of 22～34 ku. 6.5 and 7 w ere glycoproteins proved by staining with the shiff reagent and thymol/sulfuric acid. The fibrinolytic activity of 7 was highest as determined usi ng chromzym UK and chromzym PL as specific substrates.

这些组分的等电点按照它们在聚丙烯酰胺凝胶电泳图谱上的顺序从4.0开始依次降低；SDS-PAGE证明，除3、4外，其余组分均只含一种多肽链，分子质量在22～34ku之间；用shiff试剂和酚-硫酸染色，显示1、2、6.5和7是糖蛋白，其中7的糖含量最高；以BAEE、Chromozym UK和Chromozym PL为底物测定，7的纤溶酶活性最高。

Fluorophore-assisted carbohydrate electrophoresis was used to analyse pectic oligosaccharides. The influence of different factors on the separation of oligosaccharides by FACE was investigated.

荧光辅助糖电泳是首先用荧光衍生化试剂对糖类分子的还原端进行衍生化标记，然后在一定浓度的聚丙烯酰胺凝胶上进行分离的分析方法，可同时分析中性糖与酸性糖。

According to the result showed at 280nm and at 490nm,in the comparison of whether protein absorption top and sugar quantity top overlapped,glycoproteins would be detected preparatorily,and as a result,tubes in two distinct areas had glycoproteins by this method. Proteins were precipitated with trichloroaceticacid and with cold acetone,and glycoprotein was determined from SDS-gel.

再从各收集管的收集液中，用三氯乙酸沉淀蛋白法、冷丙酮沉淀蛋白法相结合浓缩、制备蛋白样品，进行SDS—PAGE，对SDS胶进行PAS糖链染色鉴定糖蛋白，并从茶树叶分级蛋白中准确地鉴定出两个区域的收集管中含有多种糖蛋白。

Cake Bakery base, vanilla cake mix, chocolate cake mix, four fondant mixes, rainbow sprinkles, white gel, gel decorator, decorating wheel, microwave baker, microwave baker lid, mixing bowl, cake mold, measuring spoon, tray, spatula, four fondant cutters, utensil holder, work mat, rolling pin, two gel cartridges, cartridge cleaning brush, four recipe cards, and instruction sheet.

蛋糕面包的基础上，混合香草蛋糕，巧克力蛋糕的组合，4翻糖混合物，彩虹洒，白色凝胶，凝胶装饰，装饰轮，微波面包，微波面包盖子，搅拌碗，蛋糕模具，测量勺，盘，铲，四达特刀具，器皿持有人，工作垫，轧脚，两个胶盒，墨盒清洁刷，4食谱卡和说明书。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。