糖胶

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

Using the MTT assay, agarose gel electrophoresis, transmission electron microscope, TUNEL method and immunohistochemistry (PCNA, bcl-2),it was studied in this experiment that the inducing APO, anti-proliferation and affection of expression of bcl-2 gene before and afterAPO of the Rubia Cordifolia L with the 5-fluorouracil(5-fu) for MGC-803 cells of gastric carcinoma.

本实验通过MTT法、琼脂糖凝胶电泳法、透射电镜观察法、TUNEL法、免疫细胞化学法（PCNA、bcl-2），研究了茜草提取物联用5-氟尿嘧啶（5-fluorouracil，5-Fu）对胃癌MGC-803细胞株的诱导凋亡、抗增殖作用及凋亡前后bcl-2基因表达的影响。

Methods Four pairs of these primers that would be amplified in simulium COI gene were designed by Primer Premier 5.0, the results of PCR would be investigated through agarose gel electrophoresis.

运用Primer Premier 5.0软件设计4对蚋科COI基因的PCR扩增引物，通过PCR扩增后进行琼脂糖凝胶电泳检测实验。

Spreng was much more simple and efficient than the conventional methods. And the extracted DNA was suitable for the polymorphic analysis based on PCR, such as ISSR-PCR.

通过琼脂糖凝胶电泳和OD260/OD280比值测定，所得基因组DNA纯度高，可作为ISSR等以PCR为基础的DNA多态性标记。

Adding purified stylar S-RNase from Prunus mume cultivar 'Gessekai' to liquid medium, for cultivating self- and cross-pollen at temperatures lot, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time.

在液体培养基中添加从果梅'月世界'花柱中纯化的S-RNase，并分别于10℃、25℃和35℃条件下培养自花、异花花粉，测定不同培养时间后的花粉萌发率和花粉管生长长度；离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后，用琼脂糖凝胶电泳检测不同温度条件下自花、异花花粉管RNA被降解的程度。

Exposure of subendothelial matrix proteins further facilitates firm attachment of platelets to the vessel wall by binding of collagen to their GPVI receptor.

内皮下基层的暴露进一步促使血小板通过糖蛋白VI受体与胶原结合使得血小板紧密的黏附在血管壁上。

Exposure of subendothelial matrix proteins further facilitates firm attachment of platelets to the essel wall by binding of collagen to their GPI receptor.

内皮下基层的暴露进一步促使血小板通过糖蛋白I受体与胶原结合使得血小板紧密的黏附在血管壁上。

To evaluate the LDL particle size and subfraction pattern and their association with coronary heart disease in Chinese population, a case-control study was conducted. Using the agarose-acrylamide composite gradient gel electrophoresis, LDL peak particle diameter was determined in 105 subjects with angiographically verified coronary artery disease and 103 matched healthy controls. Two LDL subfraction patterns were identified by the LDL peak particle diameter.

本课题以病例对照研究设计评估国人LDL颗粒大小和亚组分模式及其与冠心病关系。105例经冠状动脉造影确诊的冠心病患者和年龄性别构成匹配作为对照组的103例健康检查者的LDL峰颗粒直径，用琼脂糖聚丙烯酰胺混合凝胶电泳进行测定。

Here, by introducing C-terminally 6xHis-tagged human Nap1 gene into Escherichia coli and exploiting the nickel-charged sepharose gel system to purify the human Nap1 protein, we show that the Nap1 enzyme function is preserved in human as in lower organisms such as bacteria and plants using succinamic acid as the substrate.

此研究中，我们将已在胺基末端接上6个组胺酸标记的Nap1基因表现在大肠杆菌中，并使用接合上镍原子的琼脂糖凝胶纯化出人类Nap1蛋白，再以丁醯胺酸作为受质，证明人类Nap1蛋白具有酵素活性。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。