糖胶

Chromatinic margin was found in A549 cells after the dihydroartemisinin treatment. Apoptosis bodies could be found under the electron microscope.

双氢青蒿素作用于人肺腺癌A549细胞后琼脂糖凝胶电泳结果出现典型梯状带。

Methods In APS group and diabetes mellitus control group, levels of serum glucose, insulin, C peptide, myocardial enzymes, glycosylated serum protein and angiotensin Ⅱ in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme mRNA were detected by using fluorescent quantitative polymerize-chain-reaction; Activities of chymase and ACE were tested by radioimmunoassay.

检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平，血浆和心肌组织的AngⅡ含量；免疫组化法检测心肌Ⅰ、Ⅲ型胶原；荧光定量PCR法检测心肌chymase和ACE mRNA表达；放免法检测chymase和ACE活性。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质粒，琼脂糖凝胶电泳检测质粒，用平板稀释法测定药物最低抑菌浓度，用试管内肉汤接合法和平板表面接合法进行接合转移试验。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变；流式细胞术周期分析显示在G1期峰前存在一个凋亡峰（20.6％）；核琼脂糖凝胶（DNA 15 g.L-1）电泳呈梯状。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带，用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。