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Results Hypericin(75w tungsten lamp,10 min of light exposure,2μg/ml)had obvious cytotokic ef- feet on C6 cells, and the specific DNA...

结果金丝桃素在低剂量2μg/ml时,75W钨灯光照10min,即可对C6细胞株具有细胞毒作用;抽提其DNA做琼脂糖凝胶电泳可显示凋亡特有的DNA"阶梯"。

Objective:To investigate the effect of high glucose on the production of reactive oxygen species and type IV collagen in human mesangial cells and the intervention of tea polyphenols.

目的:探讨高糖对人肾小球系膜细胞活性氧、Ⅳ型胶原合成的影响及其茶多酚的干预作用。

In time of realizing moderate unglue hemp, it developed a series of new products like low polymeric xylose, pectin, environmental fiberboard and organic manure that reaches delamination and multilevel use of hemp's combination and disassembly.

技术特点:1、项目基于大麻纤维和麻杆等化学组成和结构的差异,开发了环境友好的汽爆新技术,为实现大麻的清洁、高效全利用奠定了基础。2、建立的大麻清洁脱胶方法,既提高了麻皮纤维的制成率和分裂度,又不损伤纤维结构,显著提高了大麻的可纺性,是一种创新的大麻脱胶技术。3、提出了利用大麻杆无胶制备环保型纤维板的方法,解决了成本高和二次污染问题。4、该项目突破依靠单一技术或单一组分利用的技术路线,按照生态工程原理,建立了大麻产品深加工利用的技术体系和综合利用新工艺,在实现麻皮纤维适度脱胶的同时,开发了低聚木糖、果胶、环保型纤维板和有机肥料等系列新产品,达到了大麻组分的分层多级利用。

Any of various mixtures of xylidine isomers.The breaker, dimethy3-phenacylthiazolium chloride, or ALT-711, can tear tough AGE bonds apart.

所谓"破坏者"即二甲基。3一苯乙基噻唑氯化物,或alt-711,它可以破坏萄糖与胶原蛋白的结合。

Objective: To investigate the ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen.

目的:观察人参糖肽对大鼠尾腱胶原交联的影响。

After cultivating in liquid medium for 5 days, an extra cellularβ-mannanase from Athelia rolfsii strain CBS191.62 was purified 15.1-fold toelectrophoretic homogeneity by ammonium sulfate precipitation, DEAESepharose Fast Flow chromatography, Hydroxylapatite chromatography andrefrigeration crystal.

产β-甘露聚糖酶的Athelia rolfsii菌株CBS 191.62,发酵培养5d,发酵液离心去菌体,上清经硫酸铵沉淀,琼脂糖凝胶层析、羟基磷灰石层析和冷冻干燥结晶等步骤,β-甘露聚糖酶的比活提高了15.1倍,获得凝胶电泳均一的蛋白样品。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况,及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用;用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料,分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA,并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Moisturizer:Water,propylene glycol,saccharide isomerate,chondrus crispus,peg-40 hydrogenated castor oil,imidazolidinyl urea,methylparaben,sodium hyaluronate,pentadecalactone,citrus aurantium dulcisoil,lactic acid,iris germanica root extract,sodium hydroxide,citrus aurantium amara oil,yellow 6(CI 15985),limonene,linalool

保湿精华:水,丙二醇,天然异构寡糖,卡拉胶,PEG-40氢化蓖麻油,尿素醛,羟苯甲酯,透明质酸钠,环十五内酯,甜橙油,乳酸,鸢尾花根萃取,氢氧化钠,苦橙油,黄 6(CI 15985),柠檬精油,芳樟醇

Compared with fresh samples, dried with silica gel, the genomic DNA was extracted from Castanea henryi leaves, which preserved for 10 days, 2 months, 4 months, 6 months, 8 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, by improved CTAB methods.

以野生锥栗幼叶为对照,采用硅胶干燥法,设置不同保存时间(10 d,2、4、6、8、10、11、12、13、14、15、16、17、18个月),对保存不同时间的锥栗叶样抽提基因组总DNA,利用紫外分光光度计及琼脂糖凝胶电泳检测提取DNA的浓度、纯度、得率及质量等指标,评价不同保存时间对基因组DNA的影响。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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