糖胶

Methods After having made excisional skin-wound on backs of iglets, we used exogenous HA gelon wounds. Then we tested the contents of GAG ,HA and collagen Ⅲ in wounds andnormal skin.

在小型猪背部的去中厚皮创面上应用不同浓度和剂型的外源性HA，并于术后第3、7、14、21、28天切取创面组织及正常皮肤，测定其糖胺多糖、HA及Ⅲ型胶原的含量变化。

Calcium chloride modified nanoparticles prepared by means of chemistry. Analyse the combination of calcium phosphate nanoparticles and DNA, the protection to DNA as well by gelose gelatin electrophoresis. When the green fluorescence protein gene was regarded as report gene, gene vector of calcium phosphate nanoparticles transfected CNE-2 cell in vitro and vivo. Combine the calcium phosphate nanopartides and suicide gene yCDg1yTK for Nasopharyngeal Carcinoma therapy in vitro.

化学方法制备，氯化钙修饰纳米颗粒，用琼脂糖凝胶电泳分析磷酸钙纳米颗粒与DNA的结合效率及对DNA的保护作用，用绿色荧光蛋白基因作报告基因，将磷酸钙纳米颗粒为基因载体转染鼻咽癌(CNE-2)细胞和在动物体内转染肿瘤细胞；以及将磷酸钙纳米与自杀基因yCDglyTK结合，并在体外对鼻咽癌进行基因治疗。

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

猪圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因，也是区分猪圆环病毒1型(PCV1)和猪圆环病毒2型(PCV2)的重要基因，本研究参照GenBank已发表的15株PCV2基因序列，设计合成一对引物，对PCV2的ORF2基因进行PCR扩增，产物经琼脂糖凝胶电泳，呈现一条大小约0.67kb的特异条带，经条件筛选，建立了猪病料中PCV2感染的PCR检测方法。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶，比活性达130.9 U／mg，纯化倍数为137.4倍；纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰，且两者完全重叠；进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色，各在负极端出现一个活性斑点和蛋白染色斑点，且两者的位置完全对应；表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

The current annual production capacity of 600 tons of soft candy plant gum, hard candy 150 tons.

现年产植物胶软糖600吨，硬糖150吨。

I was founded in 1991, is the development, production, sales of sugar gum home of professional enterprises.

我公司成立于1991年，是研发、生产、销售胶母糖的专业企业。

We will then use it to inflate marshmallows to twice their size, remove the air from them, and then shrink them down to a wrinkled, rubbery candy with the density of a gumdrop.

然后我们会用它来夸大棉花糖的两倍大小，删除的空气从他们，然后萎缩，他们到一个皱巴巴的，胶状的糖果与密度的一gumdrop 。

Hematin was immobilized on glassy carbon electrode surface by agarose hydrogel.

用循环伏安法研究了血红素-琼脂糖凝胶修饰电极在水和有机溶剂/水混合溶液中的电化学和催化还原过氧化物的性质。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。