糖杆菌

In batch cultures, glucose, xylose and arabinose in cornhusk dilute acid hydrolysate after detoxification were consumed by Actinobacillus succinogenes NJ113, when the total sugar concentration was 50 g/L in medium, the final succinic acid concentration of 34.2 g/L was obtained, resulting in a succinic acid yield of 0.68 g/g and a succinic acid productivity of 0.83 g/. When the total sugar concentration was 68.2 g/L in medium, the succinic acid concentration was 42.3g/L, the yield was 0.62g/g, and productivity was 0.98 g/.

经过脱毒处理后，产琥珀酸放线杆菌 NJ113均能利用水解液中的葡萄糖、木糖、阿拉伯糖，培养基总糖浓度为50 g/L时，丁二酸分批发酵的质量收率可达0.68 g/g，浓度可达34.2 g/L，生产强度达0.83 g/，总糖浓度为68.2 g/L时，丁二酸质量收率仍可达0.62 g/g，浓度42.3 g/L，生产强度0.98 g/。

Results: The proportion of Gram-negative bacilli rose obviously, so did the opportunistic pathogen's and Yeasts detected rate. Most drug sensitive tests showed the drug-fast rate turned to be high. Escherichia coli, Salmonella and Citrobacter resistant and so on to fluoroquinolones was 57%~97%. These bacteria to imipenem showed excellent activity. Meticillin-resistant-staphylococcus resistant to ciprofloxacin, norfloxacin, clindamycin, tetracycline, erythromycin, cefazolin and ampicillin were 40.9%, 42.4%, 63.6%, 72.2%, 85.9%, 100.0% and 100.0%, respectively Streptococcus and Enterococcus resistant to major β-lactams-penicillin, aminoglycosides, macrolides, tetracyclines and sulfonamides were between 36.0% to 100.0%.

结果：革兰阴性杆菌比例显著升高，条件致病菌和酵母样真菌检出机会显著增多；药敏结果显示多数临床细菌对常用抗生素的耐药趋势在不断上升；大肠埃希菌、沙门菌和枸橼酸杆菌等革兰阴性杆菌对喹诺酮类药物的耐药率达57%~97%，亚胺培南则对阴性杆菌有较高的敏感覆盖率；耐甲氧西林葡萄球菌对环丙沙星、诺氟沙星、氯洁霉素、四环素、红霉素、头孢唑啉和氨苄西林的耐药率分别为40.9%、42.4%、63.6%、70.2%、85.9%、100.0%和100.0%；链球菌和肠球菌对多数β内酰胺类青霉素、氨基糖苷类、大环内酯类、四环素类以及磺胺类药物均呈高度耐药，耐药率在36%~100%。

There were 70 strains isolated from each of the MRS and VJ at the different stage of the processing of dried beef. Staphylococcus warneri, Staphylococcus sciuri, Staphylococcus auricularis, Bacillus subtilis, Staphylococcus xylosus, Staphylococcus saprophyticus, Lactobacillus plantarum and Pediococcus pentosaceus were 8 predominate bacteria. The growth curve of predominate bacteria was studied. The result showed that growth of Pediococcus pentosaceus was faster than that of Lactobacillus plantarum; the growth of Staphylococcus sciuri is fastest and Staphylococcus auricularis was slowest.

在牛肉自然发酵加工过程中的不同阶段，利用MRS和PCA~+培养基分离到乳酸菌和微球菌各70株，经过分析鉴定，得到8株优势菌，分别为沃氏葡萄球菌、松鼠葡萄球菌、耳氏葡萄球菌、木糖葡萄球菌、腐生葡萄球菌、枯草芽孢杆菌、植物乳杆菌和戊糖片球菌。

Staphylococcus auricularis, Staphylococcus xylosus and Pediococcus pentosaceus had a signifient effect to the sensory quality of dried beef, Pediococcus. pentosaceu had a most signifiennt effect to improve dried beefs quality. According to all the factors, Staphylococcus xylosus, Lactobacillus plantarum and Pediococcus pentosaceus were proved to be appropriate.

结果表明：各菌株对发酵牛肉成品的pH、酸度、氨基酸总量的影响未达到显著水平；植物乳杆菌、戊糖片球菌具有增加产品中游离脂肪酸含量作用；松鼠葡萄球菌、耳氏葡萄球菌、枯草芽孢杆菌、木糖葡萄球菌、腐生葡萄球菌和戊糖片球菌则具有显著增加不同游离氨基酸的作用；从感官评价结果来看，耳氏葡萄球菌、木糖葡萄球菌、戊糖片球菌能显著影响产品的感官特性，其中戊糖片球菌能显著提高产品感官评价分值。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

A process for an enzymatic production of vitamin B6 which comprises incubating 1-deoxy-D-threo-pentulose and 4-hydroxy-L-threonine with an enzyme reaction system prepared from cells of microorganism belonging to genus Rhizobium, Sinorhizobium, Flavobacterium, Chryseobacterium, Lactobacillus, Arthrobacter, Bacillus, Klebsiella, Escherichia, Pseudomonas, Stenotrophomonas, Enterobacter, Serratia, Corynebacterium, Brevibacterium, Exiguobacterium, Saccharomyces, Yamadazma, Pichia and Candida, in the presence of NADP+, NAD+, ATP.

一种维生素B6的酶促生产方法，包括在NADP＋、NAD＋、ATP的参与下，将1－脱氧－D－苏－戊酮糖和4－羟基－L－苏氨酸与从属于以下属的微生物细胞制备的酶反应体系一起温育，所述微生物属是根瘤菌属、中华根瘤菌属、黄杆菌属、金黄杆菌属、乳杆菌属、弓形菌属、芽孢杆菌属、克雷伯氏菌属、埃希菌属、假单胞菌属、寡养单胞菌属、肠杆菌属、沙雷氏菌属、棒杆菌属、短杆菌属、微小杆菌属、酵母属、Yamadazma、毕赤酵母属和假丝酵母属。

This strain was identified to be a new subspecies of Gluconacetobacter intermedius in the species of Gluconacetobacter.

初步鉴定该菌为葡糖醋杆菌属(Gluconacetobactersp。)一个新菌种或者中间葡糖醋杆菌的一个亚种。

Meanwhile other lactic acid bacteria such as Lactobacillus plantarum, Lactobacillus bifermentum, Lactobacillus acetotolerans, Lactobacillus curvatus, Lactobacillus crispatus, Lactobacillus casei, Pediococcus pentosaceus, Pediococcus acidilactici, Leuconostoc were also identified.

同时也分离到植物乳杆菌、双发酵乳杆菌、耐酸乳杆菌、弯曲乳杆菌、卷曲乳杆菌、干酪乳杆菌、戊糖片球菌、乳酸片球菌、明串珠菌等多种乳酸细菌和德巴利氏酵母、球拟酵母等微生物。

Lactobacillus plantarum had lipolytic activity. Bacillus subtilis not only had the lipolysis but also had proteolysis.Single strains fermenting experiment showed that, the effect of different strain to the pH, acidity, the total amino acid were not signifient, Pediococcus pentosaceusand Lactobacillus plantarum could improve the content of free amino acid in dried beef.

研究结果表明：沃氏葡萄球菌、腐生葡萄球菌和植物乳杆菌只有脂肪酶活性，无蛋白酶活性；松鼠葡萄球具有弱蛋白酶活性；木糖葡萄球菌具有弱脂肪酶活性；戊糖片球菌具有脂肪酶活性，而蛋白酶活性较弱；枯草芽孢杆菌具有两种酶活性。

Ethambutol is a first-line drug used for antituberculosis therapy. It is often used in combination with isoniazid, rifampin, pyrazinamide, and streptomycin. The action of EMB in antituberculosis mechanism is the destruction of cell wall of tubercle bacilli via binding the target molecule -- arabinosyl transferases. These enzymes are involved in the polymerization of the cell wall arabinan. Inhibition of arabinan synthesis by EMB results in the accumulation of mycolic acids, leading to cell death.The EMB-resistance of MTB is involved with the mutation of emb operon or the over expression of emb operon.

乙胺丁醇是和异烟肼、利福平、链霉素、吡嗪酰胺联合治疗结核病的一线抗结核药物，EMB对结核分枝杆菌的作用机制与分枝杆菌胞壁结构破坏有关，主要作用于靶分子阿拉伯糖基转移酶，抑制了阿拉伯糖基聚合入阿拉伯半乳聚糖，从而影响细胞壁分枝菌酸-阿拉伯半乳聚糖-肽聚糖复合物的形成，导致菌细胞的死亡。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。