糖定量法

To collect the feces of patients and the control group then a quantitative analysis on Enterobacteriacea, Enterococcus, Lactobacillus, Bifidobacterium Bacteroides and Yeast was made.

采用光冈法对糖尿病患者、服用拜糖平患者及正常人群粪便中的双歧杆菌、肠杆菌、肠球菌、酵母菌进行定性、定量检测。

There are two methods to detect and quantitative analysis Neu5Ac, one is high performance liquid chromatography with the use of Aminex87H column, the other one is barbituric acid method.

对Neu5Ac的检测和定量也有两种方法，一种是利用Aminex87H酸性糖基柱，通过高效液相色谱测定；另一种是硫代巴比妥酸法测定。

Methods In APS group and diabetes mellitus control group, levels of serum glucose, insulin, C peptide, myocardial enzymes, glycosylated serum protein and angiotensin Ⅱ in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme mRNA were detected by using fluorescent quantitative polymerize-chain-reaction; Activities of chymase and ACE were tested by radioimmunoassay.

检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平，血浆和心肌组织的AngⅡ含量；免疫组化法检测心肌Ⅰ、Ⅲ型胶原；荧光定量PCR法检测心肌chymase和ACE mRNA表达；放免法检测chymase和ACE活性。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

In order to investigate regulation roles of non-esterified fatty acids,β-hydroxybuty-ric acid and glucose in hepatocyte glycometabolism and lipometabolism in dairy cows, effects of NEFA, BHBA and GLU on expression of neonatal calf glucagon receptormRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.

为阐明代谢产物非酯化脂肪酸、β-羟丁酸和葡萄糖在乳牛肝糖代谢、脂代谢中的调控作用，应用实时荧光定量PCR法观察了NEFA、BHBA和GLU对体外培养新生犊牛肝细胞胰高血糖素受体mRNA丰度的影响。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。