糖基

The current study is to test the potential of human secretor type α(1, 2) fucosyltransferase Se α(1, 2) FT］and antisense RNA transcripts complementary to the pig α(1, 3) galactosyltransferase

本文采用5型人腺病毒载体表达了人分泌型α（1，2）岩藻糖基转移酶基因和猪α（1，3）半乳糖基转移酶反义RNA，在体外用高度表达Gal α（1，3）Gal的小鼠NIH3T3细胞，体内用大鼠心脏作为模型检测了它们的表达对Gal α（1，3）Gal生成的影响。

The results demonstrate that ACM-A is capable of intercalating between the base pairs of DNA double helix, the π-π electronic overlapping between π-electron of ACM-A and DNA stabilizes the ACM-DNA adduct, and through electrostatic interaction, the trisaccharide interacts with the minor groove of DNA owing to C(3') amino group.

实验结果表明，ACM-A主要以其蒽环平面嵌入DNA双螺旋碱基对之间，发生-等电子间相互共轭，同时药物糖基上C3'位的-N(CH3)2基团带正电荷，易与带负电荷的磷酸骨架产生静电吸引，从而发生沟槽作用，嵌入DNA的小沟区，这是二者之间的主要结合模式。并且，DNA与ACM-A药物原型的亲和力要强于还原后的药物代谢产物。

Sixteen compounds were separated from 80% ethanol extract of Plant A, they were:-lariciresinol,-isolariciresinol,-isolariciresinol 9-O-β-D-glucopyranoside, tanegoside B, apiginin, apigenin 7-O-β-D-glucoside, apigenin 7-O-β-neospheroside, quercetin 3-O-β-D-glucopyranoside, 1, 7-dihydroxy-3, 4-dimethoxyxanthone, 3-O- [α-L-rhamnopyranosyl (1-4 ] -β-D-glucopyranoside-(25S)-5β-spirostan-3β-ol , 3β-O-D-glucopyranoside quinovic acid, mangiferin , 3'-p-hydroxybenzoyl-mangiferin, physcion, benzenyl methanol β-D-glucopyranoside and Vanillic acid.

结果： 1。从植物A的80％乙醇提取物中共分离得到16个化合物，分别为：-落叶松脂醇、-异落叶松脂醇、异落叶松脂醇9-O-β-D-吡喃葡萄糖苷、tanegoside B、芹菜素、芹菜素7-O-β-葡萄糖苷、芹菜素7-O-β-新橙皮糖苷、槲皮素3-O-β-D-吡喃葡萄糖苷、1，7-二羟基-3，4-二甲氧基-口山酮、菝葜皂苷元-3-O-[α-L-吡喃鼠李糖基（1-4）]-β-D-吡喃葡萄糖苷、喹诺酸3β-O-D-葡萄糖苷、知母宁、3′-对羟基苯甲酸-知母宁、大黄素甲醚、苯甲醇-β-D-吡喃葡萄糖苷、香草酸。

RESULTS: Sixteen compounds, ursolic acid (1), indole-3-carboxaldehyde (2), indole-3-carboxylic acid (3), p-coumaric acid (4), azelaic acid (5), N-trans-feruloyl tyramine (6), blumenol A (7), 2-hydroxy-3-methylanthraquinone (8), rubiadin l-methyl ether (9), rubiadin (10), quercetin (11), luteolin (12), chrysoeriol 6-C-β-boivinopyranosyl-7-O-β-glucopyranoside (13), alternanthin (14), stigmasta-5, 22-dien-3β-ol (15), and α-spinasterol (16) were obtained from the EtOAc extract of Alternanthera philoxeroides.

结果：得到并鉴定了16个化合物，分别为乌苏酸(1)、吲哚-3-甲醛(2)、吲哚-3-甲酸(3)、对-香豆酸(4)、壬二酸(5)、N-反式阿魏酰基酪胺(6)、布卢姆醇A(7)、2-羟基-3-甲基蒽醌(8)、甲基异茜草素-1-甲醚(9)、甲基异茜草素(10)、槲皮素(11)、木犀草素(12)、柯伊利素-6-C-β-波伊文糖基-7-O-β-吡喃葡萄糖苷(13)、莲子草素(14)、豆甾醇(15)和α-菠甾醇(16)。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

它们的分子中没有N-糖基化位点，不存在二硫键，很可能巯基就是分子的活性中心。

Herein, we synthesized six metalloporphyrins linked with stable nitroxyl radials, and evaluated their catalytic activity for the oxidation of glycosyl sulfides to glycosyl sulfoxides.

本论文设计、合成了6个连有稳定氮氧自由基的卟啉及其铁、锰的络合物，考察了它们对糖基硫苷等硫醚类化合物氧化成亚砜的催化性能。

It was found that manganese porphyrins linked with stable nitroxyl radials could oxidize sulfides using NaOCl as the oxidant to give the corresponding sulfoxides in good to excellent yields (80-97%) with high chemoselectivity and diasteroselectivity (dr≥7:1 for glycosyl sulfoxides).

发现连有稳定氮氧自由基的卟啉锰络合物对NaOCl选择性氧化各种硫醚底物有较好的催化效果，获得了高的产率(80％～97％)、化学选择性和非对映选择性(糖基亚砜非对映异构体的比例大于7：1)。

The protection groups in the copolymers which were prepared previously could be removed and the -NH2 and -OH groups could be reconverted. These high active groups can be used to introduce more bioactive groups and molecules so as to simulate the natural environments of ECM, so the biocompatibilities and cells adhesive capabilities between scaffolds and cells could be improved remarkably.

通过脱除共聚物糖基上的保护基团，还原出-OH、-NH_2，这些高活性的基团为进一步引入具有生物活性的功能基团，实现了对细胞外基质的人工模拟，从而调控材料的细胞识别特性，增强材料生物相容性和改善细胞与材料的黏附特性。

According to the analysis of SDS-PAGE, the bands of the graft subunits decreased and the glycosyl bands were seen more clearly with the degree of graft increasing.

聚丙烯凝胶电泳分析结果表明，随着接枝度的增加，接枝物的亚基谱带逐渐减少，糖基谱带变得明显。

Plant polyprenols, which have similar structure and biological activity to dolichols in human body, are lipids of polymer with isoprenyl units. However, dolichols are key carriers in biosynthesis process of glycosyl derivatives with protein.

植物聚戊烯醇是一类异戊烯基结构聚合体的类脂化合物，与人体内多萜醇具有相似的结构特征和生理活性，而多萜醇是糖基与蛋白质生物合成的关键载体。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。