糖基

Wheat PGIP was deglycosylated using TFMS, and the result indicates that carbohydrates content is 22%, which is consist with PGIPs from dicotyledon.

用TFMS将小麦PGIP去糖基化，结果表明小麦PGIP的糖含量为22％，与双子叶植物PGIP相似。

In this paper, one spacer- armed analogue of a disaccharide SM_3 was synthesized for the first time via multi-step synthetic method.

本文经过11步反应，首次合成了带连接臂的SM_3寡糖链，为今后合成具有更高分子量的糖基缀合物并研究其抗肿瘤免疫原性奠定了基础。

5MN/m.(2) the biosurfactant was glycolipid type which was consisted of monosaccharides, disaccharides or trisaccharides group and fatty acid group which were combined by ester bond.

2产生的生物表面活性剂为糖脂类型，糖基通过酯键与不同链长的脂肪酸相连。

Some strategies on modification of glycoside were discussed here.

提出了糖苷酶的改造方法及其在糖基化合物改性中的研究策略。

In adipose tissue and muscle, hexokinase can phosphorylate fructose to fructose 6-phosphate that then enters glycolysis.

在脂肪组织和肌肉中，己糖基酶能磷酸化果糖成为果糖6-磷酸，然后进入糖酵解途径。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The binding behavior and kinetics of uncoupling protein with its ligand, the Fourier-transformation infrared spectra of uncoupling protein and its changes during ligand-binding process, the inhibition of Guanosine 5＇-Tetraphosphate (G-4-P) and Adenine-β-Darabinofuranoside 5＇-triphosphate on the binding of uncoupling protein with GTP were studied.

本文对解偶联蛋白与配基的结合及其结合动力学，解偶联蛋白及其与配基结合过程中的傅里叶变换红外光谱及其光谱变化，鸟嘌呤核苷四磷酸（G-4-P）和9-β-D-阿拉伯糖基呋喃5＇-三磷酸对解偶联蛋白与GTP结合的影响进行了研究。

One of the important tools for glycoprotein research is lectins, which are known to have the ability to bind specific oligosaccharide moieties.

凝集素亲和色谱是蛋白质糖基化研究中很常用的工具，不同的凝集素可以对不同的单糖或寡糖有特异的富集作用。

In the present study, it has been proved that advanced glycation end products, which formated by nonenzymatic glycosylation in a long-term high blood glucose, play a important role in the loss of pericytes and development of diabetic retinopathy.

近年来的研究认为，慢性高血糖引起蛋白质非酶糖化所产生的糖基化终产物（adavaced glycation end products，AGEs），在周细胞衰亡、糖网病发生的过程中起着非常重要的作用。

Studies on four novel classes of glycosyl donors for the stereoselective synthesis of 2-S-2-deoxy-β-glycosides, via 1, 2-migration and concurrent glycosidation.

一、发展了四种糖基给体，通过硫的1，2—迁移，并同时进行糖苷化的方法，立体专一的合成了一些2位硫代β糖苷。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。