糖基

The HPLC methods for analysis of N--ribitylxylidine and N-methyl glucamine were established. After optimizing the operation conditions, the principal product and main impurities were well separated. And they were determined by LC-MS. The operation condition of N--ribitylxylidine is, columniation temperature is 30℃, flow rate is 0.7mL/min, UV wave is 244nm, the mobile phase is methanol: water=40: 60 .

首次建立了对葡甲胺和核糖胺的高效液相色谱分析方法，通过对色谱操作条件的优化，成功分离了主产物和杂质，在此条件下，应用液相色谱-质谱联用仪对糖基胺及生成杂质进行了指派定性，并测定了两种糖基胺及其主要杂质的含量，获得了满意的结果。

The changes of oligosaccharides imply the glicoconjugates, including sialic acid, galactose and fucose may play important roles during implantation.

在着床期子宫内膜各种细胞的凝集素受体糖基呈现规律性变化，提示子宫内膜糖基的变化与胚泡着床密切相关。

AIM: To investigate the effects of crocetin on receptor for advanced glycation end products mRNA expression in bovine endothelial cells induced by advanced glycation end products and the possible mechanisms involved.

目的：研究西红花酸对晚期糖基化终产物诱导牛内皮细胞中晚期糖基化终产物受体mRNA表达的抑制作用，并探讨其可能机制。

To provide a non-toxic, zero-residue and effective approach to prevent the parasitical Ascaris suum, the effect of kaempferol-3-a-L-(4-O-acetyl)-rhamnose-7-a-L-rhamnoside,extracted from Cyrtomium yunnanense, on the the animals infected with A. suum was studied.

为猪蛔虫病的防治提供无毒、无残留，对人体无害的高效防治方法，利用云南贯众分离得到的化合物山奈酚－3-a-L-（4-O-乙酰基）鼠李糖基－7-a-L-鼠李糖苷作为试验用药，对感染猪蛔虫的动物进行了药效试验研究。

During the incubation of purified α-synuclein with fructose or glucose, it was observed that the protein intrinsic fluorescence at 308 nm decreased while the fluorescence of glycated derivant at 447 nm increased.

将纯化后的α-synuclein分别与果糖和葡萄糖孵育，通过内源荧光、非酶糖基化衍生物特征荧光、圆二色光谱以及电子显微镜等技术进行检测发现：α-synuclein 与还原糖共同孵育后，308 nm内源荧光强度明显降低，同时在447 nm产生一个非酶糖基化衍生物特征荧光。

RhaG hydrolyzed only the 6-C α-rhamnoside of ginsenoside Re, RhaR is able to hydrolyze the α-rhamnoside of rutin、hesperidin and naringin.

实验结果表明，RhaG只水解人参皂苷Re的C-6位末端上的一个α-鼠李糖基，而RhaR能水解芦丁、橙皮苷、柚皮苷的α-鼠李糖基。

Objectives and methods: The present study is undertaken to examine our hypothesis that with the accumulation of ion channels, increased sialic acids at the injury site or DRG somata result in a reduction of net transmembrane potential that evoke ESD in injured neurons.

膜蛋白的一个共同特性是其膜外域接受密集的糖基化，糖基含有大量带负电的唾液酸，如大部分离子通道，特别是电压依赖性的钠通道含有大量的唾液酸，而且这些负电荷靠近钠通道的电压感受器而易化通道开放。

The results showed that Tang-an-kang could respond symptomatically, lower blood-sugar, diminish urinary protein, regulate endogenous creatinine clearance rate and lipid metabolism etc. It was suggested that Tang-an-kang had a good action on improvement lipid metabolism, diminishing urinary protein and renal function protection.

结果表明：糖安康治疗组在改善DN患者临床症状，降低血糖、糖基化血红蛋白及血肌酐，减少尿蛋白及尿白蛋白，调节内生肌酐清除率，降低血与尿β2微球蛋白，调节脂代谢，改善血液流变性等方面，疗效均优于单用西药的对照组，提示糖安康有良好改善糖脂代谢、减少尿蛋白、保护肾功能的作用。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

Lectin can be used to analyze the structures of sugarchains.

凝集素(1ectin)是一类糖结合蛋白，能专一地识别某一特定结构的单糖或寡糖中特定的糖基序列而与之结合。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。