糖基

Afterexperimental studies, the mistake in traditional TLC method was pointed out and anew simple method was established to detect uronic acid in polysaccharides.

经过实验研究，我们指出了前人的错误所在，并提出了一种简便判定多糖中有无糖醛酸残基的新方法。

In the structural studies on polysaccharides from Salvia chinensis, thetraditional TLC method to detect uronic acid in polysaccharides was found to bewrong and likely to mislead structural studies of polysaccharides.

在多糖结构研究过程中，我们发现前人所采用的判定多糖类组分中是否含有糖醛酸残基的传统TLC方法缺乏理论依据，并容易误导结构研究。

AIM To study the role of interleukin-2 IL-2 and mediobasal hypothalamus MBH in analgesia produced by Coriolus versicolor polysaccharide peptide PSP.

目的 研究白细胞介素-2 IL-2和下丘脑内侧基底部MBH在云芝糖肽PSP引起镇痛中的作用。

In this research, the complete ipt gene was cloned from Agrobacterium pTiAch 58 Hind Ⅲ fragment and its promoter was re-constructed so that following three chimaeric genes were created:(1) ipt promoter plus ipt coding region and terminator ,(2) RuBP SSU 301 promoter from petunia plus ipt coding region and terminator ,(3) pea seed-specific vicilin promoter plus ipt coding region and terminator .

将ipt基因克隆后对其启动子进行了改造，分别构建了如下三种基因：（1）ipt启动子+ipt编码区和3′区，（2）磷酸核酮糖羧化酶小亚基启动子SSU 301+ipt编码区和3′区，（3）豌豆种子特异性启动子vicilin+ipt编码区和3′区。

SPARC (secreted protein, acidic and rich in cysteine) is a multifunctional glycoprotein, which influences many important physiological and pathological processes, such as modulating cellular interaction with the extracellular matrix, abrogating cell focal adhesions, inhibiting cellular proli-feration, and regulating the activity of growth factors.

SPARC (secreted protein， acidic and rich in cysteine)即富含半胱氨酸的酸性蛋白，为一种多功能糖蛋白，在各种生理与病理过程中发挥作用，如调节细胞与细胞外基质(extracellular matrix， ECM)相互作用、溶解黏着斑完成抗黏附反应；抑制细胞增殖及调节生长因子活性等。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

The sequences of repeats and anchor nucleates are arbitrarily selected.Coupled with the separation of amplification products on a polyacrylamide or agarose gels,ISSR amplification can reveal a much larger number of fragments per primer than RAPD.It is concluded that ISSR technique provides a quick,reliable and highly informative system for DNA fingerprinting.

重复序列和锚定碱基是随机选择的，扩增产物经聚丙烯酰胺或琼脂糖凝胶电泳分离后，每个引物可以产生比RAPD方法更多的扩增片段，因此，ISSR标记是一种快速、可靠、可以提供有关基因组丰富信息的DNA指纹技术。

Langone JJ (1982) Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumococci.

其中，醛基免疫磁性载体的捕获和分离效果最佳，且用乙醛—右旋糖苷进行包被可以消除交叉反应。

The fermentative capacities of five lactic acid bacterial were studied by using saccharified black soybean .

本实验以糖化的黑豆为基质，对五株乳酸菌的发酵性能进行了测试，其中嗜酸乳杆菌产酸最高，产酸速率最快。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。