糖基

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

In this thesis, two synthetic routes for the incorporation of sugar moiety into C5(5") or C3(3") of salicylaldehyde part of Salen Mn complex were designed and several corresponding compounds were prepared, as carbohydrate derivatives are widely used in asymmetric catalytic reactions.

鉴于糖类衍生物在不对称催化反应中的广泛应用，设计了两条分别在Salen Mn配合物水杨醛部分C5(5′)、C3(3′)位并入糖基的路线并合成了相应的化合物。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分离正常人脐静脉内皮细胞，将晚期糖基化终产物修饰的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培养基与HUVEC在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面细胞间粘附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、E-选择素的表达。

The position, type and number of sugar moieties incorporated to the antibiotics have great impact on its bioactivity.

本文综述了糖基转移酶的种类、功能、特性及其在组合生物合成中的应用与研究前景。

To study the effects of glycosylation on survival of cold-storage human platelets by using rabbit model.

利用兔动物模型研究了糖基化对4℃冷藏保存人血小板体内生存的影响。

The expression patterns of expansin and XTH family genes in cucumber fruits and their responses to plant growth substances were studied.

研究了扩张蛋白和木葡聚糖内糖基转移/水解酶家族基因在黄瓜果实发育中的作用及其对植物生长物质的响应。

Abstract］ Objective To evaluate the correlation between soluble receptopr for advanced glycation end products and coronary artery disease, the etent and severity of angiographic CAD in nondiabetic patients.

目的 探讨可溶性糖基化终产物受体水平与无糖尿病冠心病的发病及病变程度的相关性。

Calnexin and calreticulin are generally known as chaperons located in the ER. However, they are also involved in multiple functions other than chaperons.

calnexin 和calreticulin 一般广为人知的功用，是位於内质网中扮演新生蛋白质的保护子，帮助蛋白质正确地进行折叠和糖基化。

Microglobulin modified with advanced glycation end products has been demonstrated to be a major constituent of amyloid fibrils.

DRA淀粉样沉积物中的主要活性成份是晚期糖基化终产物修饰的〓微球蛋白，它具有广泛的生物学活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。