糖化作用

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Ribusco-1,5-bisphosphate carboxylase/oxygenase located in the chloroplast is the most abundant protein in the leaves of light-grown plants.

1，5-二磷酸核酮糖羧化酶／加氧酶位于植物叶绿体中，是绿色植物中含量最丰富的蛋白，它催化CO2固定和光呼吸作用的第一步。

Concanavalin A is immobilized on a pre-activated chitosan microspheres, and then oriented immobilization of urease is carried out based on the strong interaction between ConA and glycoprotein.

将戊二醛将伴刀豆球蛋白和壳聚糖载体交联，然后利用ConA与脲酶糖链的特异性结合作用，实现脲酶的定向固定化。

Chitosan is a product of depolymerization and deacetylation of chitin, which has extensive biofunction because of its complex constructions.

目的：研究几丁糖对成纤维细胞增殖及Ⅲ型前胶原mRNA表达的影响，阐明其抗纤维化的作用机制。

Aminoglycosides suppress termination at nonsense codons and result in functional improvement in a cystic fibrosis bronchial epithelial cell line, in muscle cells from mdx mice and in fibroblasts from individuals with Hurler syndrome.

氨基糖苷类物质可以通过抑制无意义密码子的终止作用，改善囊性纤维化的支气管上皮细胞、患肌营养不良的肌细胞和Hurler综合征病人的成纤维细胞的功能。

For instance, ROS is involved in the hypersensitive responses, the lignification of the cell wall, the linkage of proteins and the inducible expression of many genes.

在植物抵御病原微生物的侵染过程中，ROS发挥了重要的作用，如参与超敏反应、细胞壁的木质化、富含经脯氨酸糖蛋白的交联、诱导表达抗病基因及 POD等病程相关蛋白。

Methods The viral particles of recombinant adeno-associated viral vector 2-IRES-EGFP/MnSOD(rAAV2-IRES-EGFP/MnSOD) were injected into the perilymph through the round window membrane on the 6th week.To observe the feasibility of MnSOD gene therapy for protecting the cochlear function with the methods of Westernblot, ApopTag peroxidase apoptosis detection, MnSOD activity detection and effect on hearing.

方法在半乳糖注射的第6周(注射氨基糖甙类抗生素之前2周)将rAAV2-IRES- EGFP/MnSOD病毒液经圆窗膜注入拟老化大鼠的耳蜗外淋巴，通过免疫组化(ApopTag过氧化物酶凋亡检测)、Westernblot及MnSOD活性检测的方法观察MnSOD基因在对抗内耳氧化应激损伤中的干预性保护作用。

HMF in ZXTMN group were lower than in aminoguanidine group.

止消通脉宁有阻断肾脏组织非酶糖基化的作用，而对5-HMF的作用优于氨基胍。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

OrLPT1 ORF encods a polypeptide with 535 amino acid residues (molecular mass of 57.6KDaltons), which shares more than 64% amino acid identity with some other high-affinity Pi transporters of plants. Hydropathy analysis of the deduced polypeptides showed that the OrLPT1 is highly hydrophobic and contains 12 putative membrane-spanning regions.

OrLPT1 ORF 编码长为535 个氨基酸(分子量57.6 KDaltons)的蛋白，与其他植物物种磷酸盐转运蛋白的同源性在64%以上；多肽链的疏水性分析表明，OrLPT1 具有12 个疏水的跨膜区及高亲和力磷酸盐转运蛋白中保守的3 个磷酸化作用位点：蛋白激酶C作用位点、N端糖基化部位和酪蛋白激酶II 作用部位； 3。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。