糖化

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Trehalase plays an important role in glycogen accumulation in the formarion of diapaused eggs. At the beginning of diapause, glycogen phosphorylase is the key enzyme to glycogen decomposition and the formation of sorbitol and glycerol, whereas at the stage of diapause termination, NAD-sorbitol dehydrogenase is the key enzyme to sorbitol decomposition and glycogen re-synthesis.

在滞育卵形成过程中，海藻糖酶对糖原的积累起着重要的作用；在滞育开始时，糖原的分解与山梨醇、甘油的形成，糖原磷酸化酶是关键酶；在滞育解除时，山梨醇的分解与糖原的再生成时山梨醇脱氢酶(NAD-sorbito1 dehydrogenase)是关键酶。

At present the main treatment is to increase endurance, and actively prevent and treat lung infection, and control fibrosis drug use, such as penicillamine, colchicine, azathioprine, and so on, can also be supplemented by anti-vascular inflammatory drugs, such as low-dose glucocorticoids , vasodilating agents, such as anticoagulants.

目前治疗主要是提高抵抗力，积极防治肺部感染，同时用控制纤维化的药物，如青霉胺、秋水仙碱、硫唑嘌呤等，也可辅以抗血管炎症药物，如小剂量糖皮质激素、扩血管剂、抗凝剂等。

In this research, the complete ipt gene was cloned from Agrobacterium pTiAch 58 Hind Ⅲ fragment and its promoter was re-constructed so that following three chimaeric genes were created:(1) ipt promoter plus ipt coding region and terminator ,(2) RuBP SSU 301 promoter from petunia plus ipt coding region and terminator ,(3) pea seed-specific vicilin promoter plus ipt coding region and terminator .

将ipt基因克隆后对其启动子进行了改造，分别构建了如下三种基因：（1）ipt启动子+ipt编码区和3′区，（2）磷酸核酮糖羧化酶小亚基启动子SSU 301+ipt编码区和3′区，（3）豌豆种子特异性启动子vicilin+ipt编码区和3′区。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分离正常人脐静脉内皮细胞，将晚期糖基化终产物修饰的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培养基与HUVEC在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面细胞间粘附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、E-选择素的表达。

IHC, western-blot and immunofluorescence staning revealed that the levels of ILK and ICAM-1 up-regulated 6 h after RPE cells cultured with high glucose (30 mmol/L). Real time RT-PCR analysis showed that the levels of ILK mRNA increased at 2 h, and then decreased at 6 h.

免疫组化、western-blot和免疫荧光染色都显示RPE细胞在高糖培养6 h高表达ILK、ICAM-1蛋白，而实时荧光PCR检测显示在2 h其mRNA表达量开始上调，在6 h下降。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

It is during the roasting process that the sugars and other carbohydrates within the bean become caramelized, creating a substance which is known as the coffee oil.

这样才能保证在烘焙过程中咖啡中所含的糖和其他碳水化合物碳化，从而产生出众所周知的咖啡油脂，产生出高质量、风格一致的好咖啡。

Since the establishment of the company has successfully auctioned in Guangyuan City nail Sichang, Guangyuan guesthouses, Guangyuan textile mill, the city match factory, the city of five TAC Corporation, the Urban Chinese Medicine Company, the city Department Stores Company, City of-chemical plants, North Sichuan Mica paper mills, municipal acetylene plant, Guangyuan steel door and window factory, Guangyuan City native Fruit Company, comprehensive trading company in Guangyuan City, the city agricultural resources, Bashu City Shopping Centre, City three groups of the cement plant, Guangyuan steel mills, paper mills communizing, City Tangjiu, Guangyuan gold, Cangxi Xian department stores, Jiange cement plant, Mong-owned Guangyuan Coal Mining Bureau, Wangcang mine, Wangcang rock brick factory, grinding rock mine, the South's coal mines, Peng Shan Yuan Mingfen plants, food processing plants in the Green River Shanzhen, Guangyuan Huachang machinery factory labor service companies such as Total 46 bankruptcy estate enterprises, turnover amounted to 460 million yuan.

自公司成立以来先后成功地拍卖了广元市钉丝厂、广元宾馆、广元纺织厂、市火柴厂、市五交化总公司、市医药总公司、市百货总公司、市力兴化工厂、川北云母纸厂、市乙炔厂、广元钢门窗厂、广元市土产果品公司、广元市综合贸易公司、市农资公司、市巴蜀商场、市三堆水泥厂、广元钢厂、赤化纸厂、市糖酒公司、广元金矿、苍溪县百货公司、剑阁水泥厂、广旺矿务局所属广元煤矿、旺苍煤矿、旺苍矸砖厂、磨岩煤矿、南江煤矿、彭山元明粉厂、青川山珍食品加工厂、广元华昌机械厂劳动服务公司等共计46家破产企业破产财产，成交额达4.6亿余元。

OBJECTIVE: To study the anticoagulant activity of the sulfated agarose with different relative molecular masses.

目的：观察不同相对分子质量硫酸酯化琼脂糖的抗凝血性能。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。