糖化

With α-D-glucose as raw material, 3-O-benzoyl-1,2,5,6-O-diisopropylidene-α-D-allofuranose was synthesized by isopropylene protection , oxidation, reduction and benzoylation.

以α-D-葡萄糖为原料，经异丙叉基保护、氧化、还原、苯甲酰化反应合成3-苯甲酰基-1，2，5，6-氧-二异丙叉基-α-D-呋喃阿洛糖。

In model plant Arabidopsis thaliana, also from Brassicaceae, the TRANSPARENT TESTA 12 (TT12, AtTT12) gene is seed coat-specific and encodes a vacuolar flavonoid/H+ antiporter, transporting cytoplasm-synthesized glycosylated flavan-3-ols to the vacuoles for further polymerization into seed coat pigments.

同属十字花科的模式植物拟南芥中，种皮特异表达的TT12（透明种皮12，AtTT12）基因编码一种液泡型类黄酮/H+-逆向转运蛋白，将胞浆内合成的糖苷化的黄烷-3-醇转运到液泡中，供进一步聚合形成种皮色素。

In humans, genetic defects of CathA cause galactosialidosis, a metabolic disease characterized by combined deficiency of CathA, GAL, and Neu1 and a lysosomal storage of sialylated glycoconjugates.

在人类，CathA的遗传缺失可引起半乳糖唾液酸沉积症，一种以CathA、 GAL、 Neu1不足和唾液酸化糖结合物在溶酶体的沉为特征的代谢疾病。

With substitution degree added, the colocasia esculenta schott starch phosphate ester was easier to gelatinize, the tolerance to salt and sucrose increased, the clarity first increased and then decreased, paste viscosity first decreased and then increased.

随着取代度的增加，香芋淀粉磷酸酯的糊化变得容易，耐盐和耐糖能力提高、透明度先增后减、糊粘度则先减后增。

Proliferations of cells were observed by MTT colorimetric assay and determination of total protein level in cells.

用体外制备的外源性糖基化终末产物和平滑肌细胞共孵育24h，加入不同浓度的cariporide（0.1、1.0和10μmol/L）处理。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

它们的分子中没有N-糖基化位点，不存在二硫键，很可能巯基就是分子的活性中心。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

ObjectiveTo evaluate the effect of glycation on α-crystallin molecular chaperone activity.

目的 研究糖基化对α-晶状体蛋白分子伴侣活性的作用。

In order to obtainin crystallin...

总产率是18%。将已知的结晶的2-O-对甲苯磺酰基-β-D-木糖甲基呋喃甙进行对甲苯磺酰化，得到与上述完全相同的Ⅲ，这样就证明了Ⅲ的端基的构型。

The best clarifying treatment was attempted by means of modeling and optimization with GA coupled NN. The 85% decolour rate and 80% xylitol recovery percentage was achieved.

籍助于耦联神经网络的遗传算法的模型化和优化获得了最佳的发酵液澄清方案，达到了85％脱色率和80％木糖醇回收率。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。