糖化

Low molecular weight mannuronic acid, guluronic acid and κ carrageenan were prepared from algin and carrageenan by the acid hydrolysis method, and their weight average molecular weights and the distribution widths of molecular weight were determined by high performance gel permeation chromatography respectively.

本文以褐藻胶和κ-卡拉胶为原料通过酸水解分别获得了低聚的甘露糖醛酸、古罗糖醛酸和低聚κ-卡拉胶，并采用 HPGPC法测定了这 3种低聚糖的重均分子量。在此基础上再经硫酸酯化和成盐修饰制备了各自的硫酸酯碱式铝盐。

Vitrification and devitrification tests of cryoprotectants demonstrated the feasibility of vitrification solution EDT.

在抗冻保护剂溶液的玻璃化与脱玻璃化试验中，证明了乙二醇、葡聚糖和海藻糖配制玻璃化溶液的可行性。

Wheat PGIP was deglycosylated using TFMS, and the result indicates that carbohydrates content is 22%, which is consist with PGIPs from dicotyledon.

用TFMS将小麦PGIP去糖基化，结果表明小麦PGIP的糖含量为22％，与双子叶植物PGIP相似。

Both methods combined the magnetic microspheres technology with hydrazide chemistry and Lectin, respectively. The hydrazide-functionized magnetic microspheres and GNA-functionized magnetic microspheres are prepared correspondingly. The condition for capturing glycopeptides by hydrazide-functionized magnetic microspheres and immobiling GNA onto the magnetic microspheres was evaluated and optimized, and the specificity and validity of two methods was demonstrated by using standard glycoproteins sample.

试验中分别将肼化学和凝集素与磁性微粒相结合，分别制备出了肼功能化磁粒和GNA功能化磁粒，优化了肼功能化磁粒富集糖肽的条件和GNA固定的条件，并通过标准蛋白样品对两种方法的富集特异性和有效性进行了验证。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

Green tea contains a number of flavonols with the basic structure Specific flavonols found in tea include myricetin (hydroxyls at carbon 3', 4', and 5'), quercetin (hydroxyls at positions 3' and 4'), and kaempferol (hydroxyl at position 4'), and their glycosides.

绿茶含有一些黄酮醇，其基本化学结构为：茶中特有的黄酮醇有杨梅黄酮(3'、4'、5'碳位上羟基化)、豕草花粉苷(3'和4'碳位上羟基化)，莰非醇(4'碳位上羟基化)，以及它们的糖苷物。

The functions and interactions of DNA methylation regulating factors, the establishment, mantainace and removement mechanisms of DNA methylations are reviewed in this paper.

DNA转葡糖基酶有去甲基化活性从而减轻基因沉默。文章综述了以上植物DNA甲基化调控因子的生物学功能及其之间的相互作用和近年来的研究进展，以更好的理解DNA甲基化的建立、保持和去除的机制。

The optimal derivatization condition is reducting reaction 2.5h at 50℃followed by acetylating 2.5h at 90℃.

总结出了单糖衍生化最佳的反应条件为还原温度50℃、还原时间2.5h；乙酰化温度90℃、乙酰化时间2.5h。

Thymocytes were also pretreated with or without ERKs, JNKs and p38 kinase inhibitors prior to UVB irradiation and DNA fragmentation was assessed by agarose gel electrophoresis. The activation of ERKs, JNKs, p38 kinase were investigated by western-blot.

透射电镜观察细胞超微结构的改变；琼脂糖凝胶电泳观察各组不加入或分别加入ERK抑制剂、JNK抑制剂和P38抑制剂后的DNA ladder；western-blot检测磷酸化ERK、磷酸化JNK、和磷酸化P38的活性。

Simultaneously, the activity and coding gene expression of ribulose 1, 5-bisphosphate carboxylase, phosphoenolpyruvate carboxylase, and glycollic oxidase all decrease in two rice accessions under low potassium supply while significant decrease in non-allelopathic rice "Lemont".

水稻光合作用相关酶的分析结果表明，低钾条件下核酮糖－1，5-二磷酸羧化酶、磷酸烯醇式丙酮酸羧化酶和乙醇酸氧化酶的活性及其基因表达强度均降低，但化感水稻&PI312777&的下降幅度显著小于非化感水稻&Lemont&。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。