糖化

The results showed:(1) Through glycosylated orthogonal test, When the substrate concentration is 1:2, glycosylated temperature is 60℃, the enzyme dosage is 0.5%and glycosylated time is 6 hours, glycosylating was at best, and the sugar concentration was 33%(2) through fermentation orthogonal test, when adding the yeast is 2.0%, proportion of Rhodiola and glutinous rice is 1:1, the temperature is 28℃, and fermentation time is seven days, alcoholicity of the wine was the highest and sensory evaluation was the best..

本文立足加工高品质的红景天糯米保健酒，主要对糖化条件，发酵工艺参数的确定，后发酵温度的确定，稳定性试验，膜过滤试验条件，营养成分分析等方面进行系统的研究，结果表明：(1)通过糖化正交试验，在底物浓度1∶2、糖化温度60℃、酶用量0.5％、糖化6h时糖化效果最好，糖液浓度33％；(2)通过发酵正交试验，在酵母添加量2.0％、红景天糯米比1∶1、发酵温度28℃、发酵时间7天生成的酒度最高，感官评价最好；(3)通过后发酵试验，选择10～15℃温度下后发酵，酒体澄清，感官评价最好；(4)从对红景天糯米酒稳定性分析中得知：酒体在低于-6℃时略有絮状物出现，酒体混浊沉淀的主要原因可能是蛋白质。

Steam the Ru rice familiar, add into duple water its dilution, throw in into barley bud to make its sugar turn, add just the right amount of wine song to help the sugar to turn, under the in keeping with temperature, the 35th complete the sugar turn and the wine liquid appears sweet flavor.

把蠕米蒸熟，加进二倍的水将其稀释，投放进去大麦芽使其糖化，加适量的酒曲子促进糖化，在适合的温度下，三五日就完全糖化，酒液出现甜味。

The brewhouse can mash both by batch processing and continuously.

糖化车间既能分批糖化，又能连续糖化。

This article mainly introduced completes with programmable software to the saccharification pot , the dextrinize pot, Boils the pot, the lye pot, the thermal flask, the ice watering can and the disc heat interchanger carries on the temperature xamination control, carries on the examination to the filter-tank temperature To the balance tank fluid position examination control, the thermal flask and the ice watering can fluid position carries on the examination; To wheat juice current capacity examination control, The steam current capacity carries on the measurement; To the saccharification pot, the dextrinize pot, boil the pot clamp cover steam pressure as well as the steam distributor pressure carry on the examination; To various unclear wines liquid pump, Delivers Mai Zhibeng, the mixer and various pipe-line valve according to each operating time order realization automatic control.

本课题综述了啤酒糖化过程自动化过程的软件设计，本文主要介绍了用VB语言来完成对糖化锅、糊化锅、煮沸锅、碱液罐、热水罐、冰水罐及板式换热器进行温度检测控制，对过滤槽温度进行检测；对平衡罐液位检测控制、热水罐和冰水罐液位进行检测；对麦汁流量检测控制，蒸汽流量进行计量；对糖化锅、糊化锅、煮沸锅夹套蒸汽压力以及蒸汽分配器压力进行检测；对各醪液泵、送麦汁泵、搅拌器及各管道阀门按各工作时间顺序实现自动控制。

Software to design. This article mainly introduced completes with programmable software to the saccharification pot , the dextrinize pot, Boils the pot, the lye pot, the thermal flask, the ice watering can and the disc heat interchanger carries on the temperature xamination control, carries on the examination to the filter-tank temperature To the balance tank fluid position examination control, the thermal flask and the ice watering can fluid position carries on the examination; To wheat juice 环境/给排水论文 current capacity examination control, The steam current capacity carries on the measurement; To the saccharification pot, the dextrinize pot, boil the pot clamp co 土建水利论文 ver steam pressure as well as the steam distributor pressure carry on the examination; To various unclear wines liquid pump, Delivers Mai Zhibeng, the mixer and various pipe-line valve according to each operating time order realization automatic control.

本课题综述了啤酒糖化过程自动化过程的软件设计，本文主要介绍了用VB语言来完成对糖化锅、糊化锅、工科论文煮沸锅、碱液罐、热水罐、冰水罐及板式换热器进行温度检测控制，对过滤槽温度进行检测；对平衡罐液位检测控制、热水罐和冰水罐液位进行检测；对麦汁流量检测控制，蒸汽流量进行计量；对糖化锅、糊化锅、煮沸锅夹套蒸汽机械设计压力以及蒸汽分配器压力进行检测；对各醪液泵、送麦汁泵、搅拌器及各管道阀门按各工作时间顺序实现自动控制。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Currently, most breweries grinded barley and corn together for dextrinization at 100 ℃ and then for saccharification. However, our experiments proved that it was unnecessary to dextrinize at 100 ℃ and saccharification could be operated directly without any additional enzyme.

但目前多数工厂是把大麦与玉米一同粉碎进入糊化锅100 ℃糊化，之后再进行糖化，本实验主要研究了大麦的糖化工艺，认为不需要进行100 ℃糊化，可以直接进行糖化，并且不需要外加酶。

Currently, most breweries grinded barley and corn together for dextrinization at 100℃ and then for sacchaxification. However, our experiments proved that it was unnecessary to dextrinize at 100℃ and sacchaxification could be operated directly without any additional enzyme.

但目前多数工厂是把大麦与玉米一同粉碎进入糊化锅100℃糊化，之后再进行糖化，本实验主要研究了大麦的糖化工艺，认为不需要进行100℃糊化，可以直接进行糖化，并且不需要外加酶。

With saccharified liquid of corn meal and cassava starch as a material respectively, the effects of corn steep liquor concentrations on glycerol fermentation by osmophilic yeast were discussed.

然后分别以玉米粉和木薯淀粉糖化液为原料，用耐高渗酵母发酵生产甘油，研究了玉米浆对二者甘油发酵的影响并对二者进行了比较，结果表明：当玉米粉和木薯淀粉糖化液还原糖含量分别为 2 5 %，尿素为 0 。2 %，pH为 4 。5时，用玉米粉糖化液发酵甘油时可不添加玉米浆，甘油产量最高可达 2 %，而用木薯淀粉糖化液发酵甘油时，适宜的玉米浆为 0 。15 %，甘油产量最高可达 4 。9%。

The treatment comparison by using Chinese TANG Kidney capsule and Western medicine TANG SHI PIN showed: the kidney capsule can lower the values of the blood sugar, blood fat, series of micro-protein un urine and carbon monoxide in plasma, thus the function of kidney can be improved, and prolong the process of deadness and hardening of the affected kidney small cell fibre.

利用该模型选择中药糖肾胶囊与西药糖适平对照治疗研究，结果显示：糖肾胶囊能降低糖尿病肾病大鼠的血糖、血脂、尿素氮、肌酐、尿系列微量蛋白及血一氧化氮等指标，从而减轻糖尿病肾病大鼠肾小球的高灌注高滤过，降低糖化血红蛋白含量，减轻非酶糖基化，改善肾功能，降低血脂、内皮素、调节血栓素、前列腺素水平，有抗脂质过氧化损伤的作用。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。