糖

ABSTRACT Objective To study the exprssion of Vitreoscilla hemoglobin gene in the Saccharopoblyspoura erythraea and its effect on erythromycin production.

目的 利用已含有血红蛋白基因的红色糖多孢基因工程重组菌，探讨vgb表达产物对重组糖多孢红霉菌提高红霉素产率的影响。

In S. erythraea, pZW could express reporter genes thiostrepton resistance gene and enhanced green fluorescin gene. And it is more suitable to express exogenous genes in S. erythraea stably.

在糖多孢红霉菌中，pZW可以表达硫链丝菌肽抗性基因和绿色荧光蛋白基因，是更适合在糖多孢红霉菌中稳定表达外源基因的整合型表达载体。

ErmE derived from Saccharopolyspora erythraea, which encodes a ribosome dimethyltransferase, was expressed in S. thermotolerans, to increase the substance resistance during the biotransformation of tylosin to AIV.

研究表明acyB2基因应该是碳霉素生物合成基因簇中的一个全局性正调控因子，它不仅负责对acyB1基因的正调控作用，而且还至少负责对acyA基因和红霉糖糖基转移酶基因的正调控。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质粒的启动子在糖多孢红霉菌中都不能发挥作用，故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子，并利用pSET152质粒上链霉菌染色体整合位点和氨朴霉素抗性基因，构建了表达载体pZMW。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

The production strain was inoculated into the optimum liquid and solid fermentive medium, and fermented in the optimum liquid and solid fermentation condition respectively, and then examined the GLP yield.

结果发现，固态发酵灵芝多糖的产量为58.8g/kg，液态发酵灵芝多糖的产量为52.5g/l，固态发酵的产糖量略高于液态发酵的产糖量。

The multivariate logistic stepwise regression analysis showed that compared with individuals with no drinking milk,or no fish and aquatic product intake,or no drinking tea,or no sucrose intakes,the milk intakes 250-1500 g/week(OR=0.731,95%CI:0.542-0.987),the fish and aquatic product intake with 250-1000 g/week(OR=0.720,95%CI: 0.541-0.959),or sucrose intakes 250 g/month(OR=0.446,95%CI:0.255-0.779),drinking tea forepassed(OR=0.635,95%CI:0.458-0.883),and current (OR=2.084,95%CI:1.390-3.125) had different levels of risk on MS.

Logistic逐步回归分析结果提示，牛奶摄入、水产品摄入和饮茶是代谢综合征的保护因素，而年龄和糖摄入是代谢综合征的危险因素，其中牛奶摄入在250～1500 g/周(OR=0.731，95% CI=0.542～0.987)，水产品摄入在250～1000 g/周(OR=0.720，95% CI=0.541～0.959)以及以前饮茶（OR=0.446，95% CI=0.255～0.779和现在正在饮茶(OR=0.635，95% CI=0.458～0.883)，对代谢综合征的保护作用明显，而糖摄入250 g/月时代谢综合征的患病的危险是不食用食糖的2倍(OR=2.084，95% CI=1.390～3.125)。

Four new salicylaldehyde derivatives with a glucose or mannose moiety were synthesized with 2-tert-butylphenol or 4-tert-butylphenol as the starting material, followed by the formylation, chloromethylation, nucleophilic substitution reaction with the desired carbohydrate. The salicylaldehyde derivatives condensed with ethylenediamine to give the Schiff bases, which coordinated with Mn to afford four novel sugar-based Salen Mn complexes.

首先，分别以2-叔丁基苯酚和4-叔丁基苯酚为起始原料，经过甲酰化反应、氯甲基化反应、和糖类衍生物的亲核取代反应制得了四种新型并入葡萄糖基或甘露糖基的水杨醛衍生物，将它们与乙二胺缩合生成希夫碱配体，得到的配体与过渡金属Mn~(3+)配位制备出四种新型含糖基的Salen Mn配合物。

Studies were conducted with Tochiotome strawberry cultivar (Fragaria×ananassa cv. Tohiotome) grown in a plastic greenhouse for studying the roles of sucrose and hexose metabolizing enzymes in sugar accumulation in fruit.

以设施栽培的杨乙女草莓为试材，研究蔗糖代谢和己糖代谢关键酶在草莓果实糖积累进程中的作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。