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This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻负载红细胞的影响,在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻溶液中24小时,用氰化血红蛋白试剂盒测定海藻负载红细胞的溶血率,用硫酸蒽酮法检测红细胞内海藻浓度水平。

Wild baccae of lonicera edulis was used as raw material.

用野生蓝靛果新鲜浆果为原料,经烫漂、硬化、浸、50℃渗、干燥,可制出蓝靛果低果脯;鲜果经打浆,加入白砂、氯化钙、柠檬酸、低甲氧基果胶和β-环状糊精进行控制浓缩,可制得蓝靛果低果酱。

Bacterially triggered CDDS 细菌触发型细菌触发型 CDDS The presence of azo reductase enzymes play pivotal role in the release of drug from azo bond prodrugs while glycosidase activity of the colonic microflora is responsible for liberation of drugs from glycosidic prodrugs.

偶氮还原酶溶解酶的存在在偶氮键药释放过程中发挥关键作用,结肠菌群的苷酶活性负偶氮还原酶溶解酶的存在在偶氮键药释放过程中发挥关键作用,结肠菌群的苷酶活性负溶解释放过程中发挥关键作用的苷酶活性苷药物的解放。

For D-fructose functionalized BOPP, three parameters including irradiation time, cerous concentration and hydrogen ion concentration all have positive effect on monosaccharide immobilization.

水接触角数据也揭示了酮和醛在改性效果方面的差异,酮改性的BOPP膜的表面水接触角在47°左右,而醛改性的BOPP膜的表面水接触角为60°。

The results showed that(1) UV-B irradiation can inhibit the growth and photosynthetic rate of Nitzschia closterium,and the inhibiting effects were more significant when the time was prolonged;(2) UV-B irradiation decreased soluble sugar.In the initial 2 days,soluble sugar of N.closterium decreased rapidly,the soluble sugar on the 4~ day has...

结果表明:(1)随着UV-B暴露时间的延长,UV-B辐射对藻的生长以及光合速率有抑制作用,并且时间越长,抑制作用越明显;(2)UV-B辐射使小新月菱形藻可溶性含量降低,而且在最初的2d,UV-B辐射使可溶性含量降低迅速,第4天的可溶性含量稍有所升高但与第2天相比差异不显著,UV-B辐射第6天,可溶性含量又迅速降低;(3)UV-B辐射的可溶性蛋白含量在第2天明显升高,随后又快速下降;(4)对照组的还原型谷胱甘肽含量的变化随时间延长而升高,UV-B辐射第2天的GSH含量比初始状态(0d)的GSH含量稍有升高,但变化不明显,第2天之后,GSH含量随时间延长而快速降低;(5)对照组的过氧化物酶活性随时间延长而升高,UV-B辐射第2天的POD活性比初始状态(0d)的POD活性显著升高,第2天之后,POD活性随时间延长而快速降低。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析,以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量,并探讨了两种分析方法的精密度。911分子没有紫外吸收,为提高检测灵敏度,本文采用荧光标记法,通过对三种荧光试剂与几种寡和多衍生效果的综合比较,最终确定了盐酸胍为最佳衍生试剂,并以911为代表详细探讨了衍生化反应的最优条件。911链结构与褐藻胶主链结构类似,以甘露醛酸和古罗醛酸的嵌段形式存在,分子中M/G比值与911的物理化学性质和生物活性有直接关系。

One is serious environmental pollution and high cost caused by pretreatment of materials,the other is the lower conversional rate of fuel ethanol during sugar fermentation.I think these problems can be solved through two ways.

作者认为采用生物降解原料中木质素的预处理工艺和采用戊发酵的微生物菌种来同步发酵化醪液中戊和己的工艺是解决生物质纤维燃料乙醇生产的重要途径。

We found that under acid condition, selenite radical combined with active hydroxide radical and amino-group of chitooligosaccharides to form chitooligosaccharides - Se compound molecule, the remaining group complex interacted with the negative sodium tripolyphosphate via electrostatical interactation to form nanoparticles with the size about 100nm.

通过透射电镜、粒径分析仪等方法表征,发现壳寡-硒纳米颗粒平均粒径为100nm左右,在酸性条件下,亚硒酸根与壳寡单元上活性羟基和氨基化合,形成壳寡-硒复合分子,剩余的基团与带负电的多聚磷酸钠复凝聚,在4℃下能够稳定保存,25℃以上壳寡溶胀而致纳米颗粒发生分解。

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated by high performance liquid chromatography with differential refraction detection basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths.

通过高效液相色谱/示差折光检测系统分析可获得细菌D-97利用糊精或淀粉水解物合成海藻的基本生物学信息,包括微生物培养碳源对细菌D-97胞内海藻合成酶系的影响以及该酶系利用不同种类或不同分子链长度的麦芽寡合成海藻的能力及作用过程。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明:(1)在膜透性的研究中,丛毛羊胡子草抗寒性最强,采用电导率法测得半致死温度LT_(50最低,丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃;在可溶性蛋白含量研究中,抗寒锻炼前后三种植物的可溶性蛋白含量均增加,同时采用SDS-PAGE法,通过对蛋白条带分析,以丛毛羊胡子草条带数最多且着色深;(2)在可溶性的研究中,三种植物经抗寒锻炼后叶片可溶性含量都比抗寒锻炼前高,且经过抗寒锻炼后三种植物可溶性均呈下降趋势,丛毛羊胡子草可溶性含量下降较小;从游离脯氨酸含量可知,低温的胁迫影响了植物叶片游离脯氨酸的含量变化,植物的抗寒性与游离脯氨酸有关;低温胁迫下三种植物叶绿素含量随着温度的下降,叶绿素含量、叶绿素a、叶绿素b和叶绿素a/b均呈下降趋势,狗牙根对低温胁迫最为敏感;(3)低温锻炼前后,丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的,特别是过氧化物酶,说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。