糊精酶

The optimum technological conditions has been obtained as follow: under the conditions of liquefaction temperature 105℃, enzyme decompound temperature 92℃, addition of 0.1~0.2 g/kg (20000 U), hydrolysis time 40~50 min, concentration of substrate 15%~20%, maltodextrin production DE can less than 7.0. The content of keeping transparence has been studied, provided a strong foundation to industry production.

通过正交实验，确定了生产低DE值麦芽糊精的最佳工艺条件为：液化温度105℃，酶解温度92℃，酶用量0.1~0.2g/kg干物，酶解时间40~50 min，淀粉浆质量分数15%~20%，麦芽糊精的DE值达到7.0以下，并对低DE值麦芽糊精保持透明度提出了组分要求和最高浓度要求，为其工业化生产打下了坚实的基础。

Meanwhile cyclodextrin can simulate ester hydrolase,which also may have an impact on itself.Up to now,there is no research focused on the stability of cyclodextrin ester.

同时环糊精本身就可以模拟酯解酶，也有可能对自身酯键产生影响，目前还没有专门真对环糊精酯键稳定性的相关研究。

In this study,26 wheat cultivars were used as experimental material to study the Changes of limit dextrinase during wheat malt production.

以26个小麦品种为材料，研究了不同品种小麦芽制备过程中极限糊精酶活力的变化。

Limit dextrinase is a key enzyme in determining the malting quality.

极限糊精酶是决定麦芽品质的关键酶。

of stained sustrate was used to test activity of limit dextrinase extracted from Jipi2 malted for 1 - 7 days.

利用染色底物分析法对发芽1-7天的大麦品种吉啤2号麦芽中的极限糊精酶活性进行检测。

Changes of DBE activity of four cultivars had a single-peak curve, and the peak appeared in 14 d after anthesis. HPLC analysis showed that the activity of DBE to amylopectin was similar. There are two types of DBE in the wheat grains, one is isoamylase, and the other is limit dextrinase.

在灌浆过程中，淀粉去分支酶的活性均呈单峰曲线变化，灌浆中期活性最大；淀粉去分支酶对底物支链淀粉均具有相似的酶解活性；对灌浆中期小麦淀粉去分支酶进行的纯化结果表明，小麦籽粒胚乳中也存在2种类型的淀粉去分支酶，一种是以极限糊精为特异性底物的极限糊精酶，另一种是以支链淀粉为底物的异淀粉酶。

Compared to the European barley variety Triumph, the levels of enzymatic activity and thermostability of limit dextrinase ost Chinese barley varieties are low, except for ZDM1616, which is nearly equal to Triumph.

同Triumph相比，供试的20个国产大麦极限糊精酶活性、耐热性普遍较低，仅ZDM1616与Triumph略为接近。

The quality cooking oil was encapsulated by the composite wall materials of the soybean residues' fiber solubly enzymatic hudrolyzates and malt dextrin, the ratio of which was from 2:1 to 4:1. The products had better qualities including appearance, solubility, superficial oil, and the ratio of embedding, fluidity and so on. And the products had a blithe oral feeling, no peculiar smell and had a little sweet taste.

豆渣纤维素水溶性酶解产物与麦芽糊精以2：1～4：1之间的比例作为复合壁材对液体调和油进行包埋，产品在外观、溶解性、表面油含量、包埋率、流动性等方面与比麦芽糊精为壁材的产品更优；该产品口感清新，无异味，带有微微的甜味，爽口。

Times higher than that of initial strain. Polysaccharides, such as barley flour, dextrin and soluble starch, were better carbon sources than monosaccharides and disaccharides, such as glucose and maltose, for cell growth of B subtilis ZJF-1A5 and β-glucanase production. Yeast extract was the best nitrogen source, the following was soybean flour.

糊精、大麦粉等多糖有利于B.subtilisZJF-1A5 β-葡聚糖酶的产生，葡萄糖、蔗糖等易利用性碳源不利于菌体生物量的积累和β-葡聚糖酶的产生，β-葡聚糖酶受分解代谢产物阻遏调节。B.subtilis ZJF-1A5 β-1，3-1，4-葡聚糖酶的产生与菌体生物量和菌体生长状态密切相关，提高发酵产酶水平的关键是提高菌体生物量和解除葡萄糖效应。

The preparation of immobilized enzyme: The inulinase, used in the fructose production from Jerusalem artichokes, and the glucoamylase, used in the glucose production from dextrine were chosen as samples. The comparative tests were carried out on specially designed setups for the immobilization of enzymes, and a synthetic evaluation was made of the quality of the immobilized enzymes.

固定化酶制备方法的选择：选定了降解洋姜液生产果糖工艺的菊糖酶及降解糊精生产葡萄糖工艺的糖化酶分别进行了不同方法的酶的固定化；设置了相应的实验装置，进行对比实验，研究了各因素对酶的固定化的影响；并对所制备的固定化酶的效果进行了综合评价。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失