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精氨酸

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The composition of amino in feed matter and feed draff after degradation for sixteen in rumen compared, from which we found the content of vary of amino acids in peanut meal and Soybean meal had increasing trends after sixteen hours cultured in rumen. The increasing trends of leucine, isoleucine, threonine, phenylalanine were greater. The increase trends of lysine, methionine, arginine and histidine were smaller. The content of varies of amino acid in cotton seed meal matter had decreasing trend s after sixteen hours cultured in rumen, but decreasing value was small. The compositions of amino acid in lees and rape seed meal matter were steady after sixteen hours cultured in rumen.

通过把饲料原料中氨基酸的组成和瘤胃降解16h后饲料残渣的氨基酸组成比较发现各种蛋白质饲料瘤胃降解前后氨基酸组成均发生了一定的变化,总的来说,氨基酸浓度在降解后有上升趋势(p<0.05)的有亮氨酸、异亮氨酸、苯丙氨酸、缬氨酸、苏氨酸、丙氨酸和脯氨酸,氨基酸浓度在降解后有下降趋势(p<0.05)的有赖氨酸、精氨酸、谷氨酸和胱氨酸,氨基酸浓度在降解后差异不显著的有组氨酸、天门冬氨酸、甘氨酸,降解后无明显规律的有蛋氨酸、丝氨酸和酪氨酸。

Development period of the parasitoid pupa from the 5th instar larva fed on MSB was significantly longer. Length of cocoon of MSB treatment was significantly shorter than the control. However, no obvious differences were detected in the development periods of egg to larva, cocoon mass size, wasp emergence rate, sex ratio, longevity and forewing length of wasp between KMD1 and the control. 3. Amino acid content in the haemolymph of SSB after fed with transgenic rice Although there were no changes on the components of amino acids in the haemolymph of SSB after fed with two transgenic rice lines, there were some changes on relative contents of amino acids.

取食KMD1 36h后0天,20种氨基酸中除天门冬酰胺、丝氨酸、苏氨酸和甲硫氨酸外,其它16种氨基酸的含量都高于对照,其中组氨酸、甘氨酸、苯丙氨酸、异亮氨酸、赖氨酸差异达显著水平,谷氨酰胺、脯氨酸差异达极显著水平,与氨基酸总量表现一致的变化趋势;取食KMD1后1天,除天门冬酰胺、谷氨酰胺和赖氨酸高于对照外,其它17种氨基酸均较对照低,但仅丝氨酸、苏氨酸和色氨酸与对照差异显著;取食KMD1后3天,绝大多数氨基酸含量的变化与取食KMD1后0天的趋势一致,除丙氨酸和色氨酸比对照稍低,其它18种氨基酸均高于对照,其中精氨酸、缬氨酸差异显著,丝氨酸差异达极显著。

Shanghai Kyowa Amino Acid Co., Ltd. is a Sino-Japan joint venture found ed by Guanshengyuan Co.,Ltd and Kyowa Hakko Kogyo Co.,Ltd.

现公司最新供应以下产品: L-丙氨酸、L-精氨酸、L-盐酸精氨酸、L-门冬氨酸、L-谷氨酸、L-组氨酸、L-盐酸组氨酸、L-盐酸赖氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸等。

The contents of putrescine and spermidine increased more obviously in high responding line and decreased significantly in low responding lines.

敏感型材料的游离脯氨酸、丝氨酸、精氨酸、丙氨酸含量水平明显比迟钝型材料的高。25d低温预处理后,敏感型材料的多胺总量比0d时的明显提高,腐胺和亚精胺的含量升幅特别显著;迟钝型材料的多胺总量比0d时的低,亚精胺和精胺的降幅较为明显。

Xinjiang medicine's content of amino acid of fruit juice of mulberry fruit is concrete: 2,6-diaminocaproic acid 0.089, serine 0.044, histidine 0.025, glycine 0.049, ammonia 0.088, valine 0.057, 2-amino-5-guanidinovaleric acid 0.051, isoleucine 0.042, Radix Asparagi 0.068, 0.020, threonine 0.076, 0.022, 0.128, 0.069, third 0.048, benzene third 0.042 leucine glutaminic acid methionine hydroxyphenylaminopropionic acid.

新疆药桑椹果汁氨基酸含量具体为:赖氨酸0.089、丝氨酸0.044、组氨酸0.025、甘氨酸0.049、氨0.088、缬氨酸0.057、精氨酸0.051、异亮氨酸0.042、天门冬氨酸0.068、酪氨酸0.020、苏氨酸0.076、蛋氨酸0.022 、谷氨酸0.128、亮氨酸0.069、丙氨酸0.048、苯丙氨酸0.042。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-精氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-精氨酸初始浓度对苯甲醛沉淀L-精氨酸的影响,结果表明,沉淀反应的适宜pH值大于11,苯甲醛与L-精氨酸的适宜摩尔比为1.25,(来源:Aa6BC论文网www.abclunwen.com)L-精氨酸适宜的初始浓度在15g/L以上,氯化钠的存在对沉淀率基本上没有影响,而氯化铵和L-赖氨酸的存在使沉淀率下降;同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线,表明其最大平衡吸附量为30mg/g,考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响,结果显示从L-精氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2,温度对L-赖氨酸吸附率影响不大,吸附过程可在室温下进行,氯离子的存在使L-赖氨酸的吸附率降低。

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果:1、热应激后小鼠的各项免疫指标均有显著性下降,其中在2.8小时降到最低,提示在这个时间段小鼠的免疫功能受到最大损害;2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩;3、常温组,补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升,而热应激后三指标均显著降低,其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少,提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制;4、补充精氨酸组的活化胸腺细胞[Ca~(2+)]较给水组有显著性上升,说明精氨酸对热应激小鼠胸腺细胞具有保护作用,并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大;5、热应激后小鼠血清NO的浓度均有不同程度的上升,补充精氨酸后血清NO上升更加明显,但并非随精氨酸给予浓度的增加而增加。

It can convert arginine, a semi-necessary amino acid, into citrulline and ammonia. And the absence of arginine can restrain the growth of cell.

精氨酸脱亚氨基酶可把非必需氨基酸——精氨酸转变为瓜氨酸,而精氨酸的缺失将使细胞生长受到抑制。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

In metabolizing argine, there had no obvious difference among three strains and the control 3 1 DH. Thus, three selected strains had better characters of degrading argininc that they couldn"t over-degrade arginine to produce large numbers" precursors of carcinogenic ethyl carbamate as citrulline, etc.

分析表明,精氨酸分解代谢方面,三个菌株与对照31DH没有明显差异,由此说明,三个优选菌株精氨酸代谢特性良好,不会过多降解精氨酸、代谢产生瓜氨酸等前体物质,从而不会导致致癌物质——氨基甲酸乙酯的大量合成。

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