精母细胞的

Methods Male infertility couples from October 2006 to July 2008 were chosen and divided into ICSI group and TESA+ICSI group. ICSI group were 584 oligozoospermia cases with alive normal sperm in spermatic fluid. TESA+ICSI group were 54 obstructive azoospermia cases with alive normal sperm in testicle. Two groups were treated with ICSI after wives obtained oocytes by ovarian stimulation.

选择2006年10月～2008年7月因男性不孕症夫妇行ICSI治疗的患者638例分成两组，ICSI组和TESA+ICSI组，ICSI组为自行排精后精液内可以找到正常形态活精子的少精症患者行ICSI治疗584例，TESA+ICSI组为梗阻性无精子症患者经睾丸穿刺找到睾丸内形态正常活精子行ICSI治疗共54例，两组女方均给予超促排卵用药获取卵母细胞。

GA(subscript 1+4) distribution in microspore mother cells, uninucleate and binucleate pollen, especially in nucleus, gradually increased with the anther development. Moreover, some GA(subscript 1+4) distributed in sperms.

短日照条件下花药可育，花粉母细胞、单核花粉和二核花粉内GA(下标 1+4)的分布数量逐浙增多，以细胞核最为明显；成熟花粉的精细胞内也有一定数量的GA(下标 1+4)分布。

In oocytes matured in vitro for 48h, polyspermy ratio is 73%, and lower than that in 36h(83%)and 24h(80%).

结果显示，体外成熟48h的卵母检测细胞其多精受精率为73%，比体外成熟36h(83%)、24h(80%)的卵母检测细胞要低。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

In the present study, the immunocytochemical localization of ER of termite gonads were investigated with SABC method.

结果表明，ER在末龄若虫期的卵巢和精巢中都存在，免疫阳性物质定位在分化期和生长期的卵母细胞以及精原细胞中。

It is necessary to develop a protein-free media for oocytes maturation that can prevent zona hardening. In this study, immature macaque oocytes were cultured in modified CMRL media alone or CMRL with either bovine calf serum or PVA for 24 hours. Also, in vivo matured oocytes were cultured in CMRL supplemented with PVA or serum for approximately 6 hours.

我们在本研究中，将非成熟的猕猴卵母细胞分别在改良的CMRL，或在含有胎牛血清或聚乙烯醇的改良CMRL培养基中培养24小时；同时，将体内成熟的卵母细胞分别在含有PVA或者胎牛血清的CMRL中培养6小时，然后进行体外授精，比较各组间精子穿透率或受精，以及胚胎发育情况。

Four main factors which possibly affected sex were described such as arginine concentration in female animal vagina, glucose in the uterus, maturational status of the oocyte at the time they encounter sperm as well as the hormone levels of both parents at the time of conception.

这些环境因素包括母体生殖道中精氨酸含量、胚胎发育过程中子宫内葡萄糖浓度、输精时卵母细胞成熟程度以及哺乳动物受孕时生殖激素的水平等方面，为进一步开展性别控制研究积累一定的资料。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。