精母细胞的

Centromeres could be identified easily on the bivalents at diplotene, so it was convenient to analyse the karyotype.

结果表明，日本沼虾染色体数n=52，2n=104，据双线期二价体的相对长度、着丝点位置以及形态诸方面的特点分析了核型，共分为A，B，C，D4个染色体组，核型组成是N=37M+4ST+11T；初级精母细胞减数分裂前期I可分为细线期/偶线期、粗线期、双线期和终变期5个时期，双线期的二价体存在弥散阶段，核仁明显，且双线期二价体着丝点清晰，给核型分析带来了方便。

To elucidate the relationship of GSE expression and gametogenesis, mRNA (0.5μg each) from mouse testis and ovary on different postnatal days were analyzed by RT-PCR with specific primers for GSE cDNA. The GSE mRNA in the testis of mouse was first detected at Day 14 postpartum, when cells at mid-pachytene are likely to appear.

为了阐明GSE表达与配子发生的关系，从0～49日龄小鼠睾丸和卵巢中提取mRNA（每样品0.5μg，将其与特定的GSE cDNA引物进行RT-PCR反应，分析的结果发现，GSE mRNA在出生后第14天的小鼠睾丸中首先被检测到，这一时间正是中粗线期精母细胞很可能出现的时期。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅，属鲈形目、刺鳅科(Perciformes，Mastacembelidae)，是广泛分布于中国南方的小型淡水鱼类。1998年，我室通过核型分析，首次发现该物种具有明显分化的异形性染色体，其性别决定类型为XX／XY型。1999年，本室通过染色体显带、精母细胞联会复合体观察，再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体，两者不仅已经出现结构异染色质分布上的不同，同时在外部形态上也存在明显差异，识别特征鲜明，在光镜观察下无需显带即能清晰辨认性染色体。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作，建立卵胞质内圆形精细胞显微注射受精技术程序，探索猪圆形精细胞显微受精的可能性，研究影响这种特殊细胞显微注射受精效果的因素，为进一步研究猪ROSI技术提供基础资料。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响，以探索猪卵母细胞体外成熟规律，提高体外培养成熟度，为ROSI技术体系准备优质卵母细胞；然后针对猪圆形精细胞的体外分离进行了试验，进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection，ICSI)操作的比较研究了影响ROSI操作的因素。

In oocytes matured in vitro for 48h, polyspermy ratio is 73%, and lower than that in 36h(83%)and 24h(80%).

结果显示，体外成熟48h的卵母细胞其多精受精率为73%，比体外成熟36h(83%)、24h(80%)的卵母细胞要低。

For further studying the genetic mechanism of the reproductive isolation phenomenon of interspecific hybrid, paraffin sections of testes from 13 sexually mature male mules (Equus asinus×Equus caballus) and 12 hinnies (Equus caballus×Equus asinus) were prepared, and the samples of Giemsa meiosis and AgNO 3 synaptonemal complexes were observed and compared with spermatocyte meiosis of horse and donkey under light microscope and electronic microscope.

为了进一步探讨种间杂种生殖隔离现象的遗传机理，本研究采用性成熟公骡的睾丸为材料，制备组织切片和减数分裂联会复合体标本进行观察，发现个别性腺发育较好的个体虽然减数分裂存在大量异常，但确有少数精母细胞完成了减数分裂并产生精子。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。