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Bcl-2 positive signals had no obviously changes in cryptorchid testis on 3d, but fluorescent signals of Bcl-2 protein decreased obviously in cryptorchid testis on 6-15d with the germ cell apoptosis increased.

随着隐睾诱导生精细胞凋亡增加,Bcl-2蛋白的表达明显下降,呈下调趋势;而Bax蛋白的表达明显增加,呈上调趋势,尤以精母细胞胞质更为显著。

Obviously, large amounts of cyclin B1 existing in spermatids has no effect on adjusting the activity of 〓 kinase, which may be just a signal of the end of cell division and/or a necessary factor for the initiation of the metamorphosis of spermatids. Abdominal high temperature inhibit efficiently the translation of cyclin B1 and 〓 in the spermatogonia and pachytene/diplotene primary spermatocytes.

显然,精子细胞中大量存在的cyclin B1蛋白不会起到调节〓激酶活性的作用,而是作为一种细胞分裂完成的信号,为启动精子细胞变态所必需;(4)腹腔高温使粗线期/双线期初级精母细胞中cdc2和cyclin B1转录本的翻译受阻。

On the D110, when mean total length reached about 86.5±5.9 mm, ovarian cavity grew bigger, and oocytes were firstly detected. Differentiation of testis was firstly observed in juvenile flounder with mean total length of 63.5±3.4 mm on the D90 when spermatogenous cell proliferated rapidly and spermaduct formed.

在90日龄、平均全长为63.5±3.4 mm的雄性牙鲆中,精原细胞快速增殖,并观察到了输精管结构;进一步的细胞学分化则出现在100日龄、平均全长为76.0±8.6 mm的个体中,此时可以看到精小叶的形成;在平均全长为140.0±15.2 mm时,精巢中出现初级精母细胞,标志着性腺分化的基本完成。

In male, the immunopositive reaction in spermatogonia, spermatocyte and spermatid to testosterone antibodies strengthened gradually during the development and maturation of testis.

在大生长期和成熟期,精原细胞和精母细胞对孕酮抗体发生强的免疫阳性反应。

Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. It is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on.

除精子细胞的核蛋白不完全成熟及卵子激活不足等因素外,如何正确选择存活精子细胞是个难题,如圆形精子细胞与精母细胞、单核细胞和多形核白细胞等其他圆形细胞的区分就比较困难。

These results indicated that CB is necessary for synchronization in spindle formation in both oocyte and primary spermatocyte, and that synchronization may be required for subsequent embryo development.

这一结果表明CB的处理可以使精母细胞与卵母细胞同步形成MI纺锤体,使其发育同步,这可能对以后胚胎的发育具有重要作用。

Previous studies have demonstrated that primary spermatocyte can undergo meiotic divisions in MI oocytes and the resulting embryos can develop to term. However, the success rate is very poor.

已经证明初级精母细胞在显微融入MI卵母细胞后可以完成减数分裂并且产生的胚胎可以发育到期,只是发育率还非常低,作为一种形式的核移植,细胞周期的同步化处理对于胚胎的发育具有重要意义。

Immunohistochemistry for Bax: Bax expression was detected in germ cell cytoplasm cytoplasm and nucleus of seminiferous tubule theca cell, obviously spermatocyte.

免疫组化观察Bax的表达情况:Bax表达见于生精细胞的胞质和胞核,以精母细胞为主。

Sinensis could be divided into 5 stages, includi ng t he spermospore proliferation stage, the primary spermatocyte stage, the secondar y spermatocyte stage, the spermateliosis stage and the spermiotiliosis stage.

在整个精子发生过程中,N-乙酰半乳糖复合物出现于精原细胞期,并于精母细胞期发生明显的修饰和变化,精子形成期和成熟期没有N-乙酰半乳糖复合物的表达。

In male, three kinds of sex steroid receptors were distributed in the spermatogonia, spermatocytes and spermatids, and the positive reaction was localized in the cytoplasm and nucleus of these spermatogenic cells.

在雄性,3种类固醇激素受体分布在不同发育时期的精原细胞、精母细胞和精子细胞中,受体阳性物定位在胞质和核。

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