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Centromeres could be identified easily on the bivalents at diplotene, so it was convenient to analyse the karyotype.

结果表明,日本沼虾染色体数n=52,2n=104,据双线期二价体的相对长度、着丝点位置以及形态诸方面的特点分析了核型,共分为A,B,C,D4个染色体组,核型组成是N=37M+4ST+11T;初级精母细胞减数分裂前期I可分为细线期/偶线期、粗线期、双线期和终变期5个时期,双线期的二价体存在弥散阶段,核仁明显,且双线期二价体着丝点清晰,给核型分析带来了方便。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩,管壁增厚呈空虚网状,生精细胞层次减少,间质细胞增生,但仍见有精子生成;高剂量组可见到精子呈异型性改变,细胞核浓缩深染,且随着摄入时间延长改变愈趋明显;F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩,间质增生明显,20个月时肾小球萎缩纤维化;F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生,淋巴母细胞增生活跃,20个月时脾小体减少,生发中心稀疏;F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润,晚期骨髓有核细胞减少,脂肪细胞增加。

Polyamines including putrescine, spermidine, and spermine play important regulatory roles in mammalian cell proliferation and differentiaton.

多胺包括腐胺、精脒及精胺,在真核细胞增殖与分化过程中起着重要的调节作用。

In addition, the silica-precipitating and templating roles of protamine were elucidated, and the mediation mechanism of organic molecules in biomimetic silicification was also tentatively analyzed.

最终确定以羧甲基纤维素钠为仿生微囊的液核,制备出具有海藻酸/精蛋白壳膜和精蛋白/氧化硅杂化壳壁的微囊,并用于包埋GUS,催化黄芩苷转化为黄芩素。

Immunohistochemistry for Bax: Bax expression was detected in germ cell cytoplasm cytoplasm and nucleus of seminiferous tubule theca cell, obviously spermatocyte.

免疫组化观察Bax的表达情况:Bax表达见于生精细胞的胞质和胞核,以精母细胞为主。

Immunohistochemistry for HIF-1α: HIF-1αexpression was detected in germ cell cytoplasm cytoplasm, obviously spermatocyte,and was detected in nucleus of seminiferous tubule theca cell too.

免疫组化观察HIF-1α的表达情况: HIF-1α表达主要见于生精细胞的细胞质,以精母细胞明显,也见于曲细精管外周膜细胞的胞核。

The changes were observed by microinjecting Larginine, Darginine, sodium nitroprusside and hemoglobin into rat caudate nucleus in 30 minuts. Results:The significant hyperalgesic effects were observed by microinjecting LArg and SNP into caudate nucleus of rats.

以钾离子透入引起大鼠甩尾的电流强度作为痛反应指标,尾核内微量注射L精氨酸、D精氨酸、硝普钠和血红蛋白等,观察0~30min内大鼠痛阈的变化。

G banding karyotype analysis of peripheral lymphocytes in 217 patients with azoospermia or severe oligospermia were performed and the Y-chromosome AZFc region from seven cases with Y chromosome abnormality was amplified by polymerase chain reaction.

对217例无精和严重少精症患者外周血淋巴细胞染色体核型进行分析,并采用聚合酶链反应对7例Y染色体结构异常患者的AZFc区进行检测。

G banding karyotype analysis of peripheral lymphocytes in 217 patients with azoospermia or severe oligospermia were performed and the polymerase chain reactions for AZFc of Y-chromosome in the blood from 7 cases with Y chromosome abnormality.

对217例无精和严重少精症患者外周血淋巴细胞染色体核型进行分析,并采用聚合酶链反应对7例Y染色体结构异常患者的AZFc区进行检测。

In male, three kinds of sex steroid receptors were distributed in the spermatogonia, spermatocytes and spermatids, and the positive reaction was localized in the cytoplasm and nucleus of these spermatogenic cells.

在雄性,3种类固醇激素受体分布在不同发育时期的精原细胞、精母细胞和精子细胞中,受体阳性物定位在胞质和核。

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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