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The measurement of the ability of sperm to undergo the acrosome reaction is conventionally used for assessment of sperm capacitation.

检测精子是否具有顶体反应的能力是评估精子获能的一种常规方式。

Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction.

Western blot和双染的研究表明在精子发生获能和顶体反应的过程中NYD-SP27逐渐从精子上丢失。

Sperm capacitation in vitro and acrosome reaction were premise and basic of IVF. So study on sperm capacitation and AR helps to the development of IVF.

精子体外获能和顶体反应是体外受精的前提和基础,因此对精子体外获能和顶体反应进行研究有助于体外受精技术的深入开展。

Objective To investigate the relationship between ovum fertilization rate and sperm morphology, acrosome reaction in IVF and to explore the influence of parameters of sperm on fertilization rate.

目的 研究体外受精(in vitro fertilization,IVF)中精子形态、顶体反应与卵子受精率之间的关系,分析精子各参数在IVF受精率方面的参考意义。

Pregnancy suppression in the rat with an antiserum to lactate dehydrogenase-C4 is reported in this paper. The antibody was proven to be a sperm agglutinin.

LDHeeO'是一种精子特异的乳酸脱氢酶同工酶,也是第一种既能高度纯化,又具有免疫抗生育作用的精子特异抗原。

It is revealed that the aberration of sex chromosome causes the most serious spermatogenesis failure and some breakpoints of autosome may also affect spermatogenesis.

由此可见,性染色体数目和结构异常是精子发生障碍的主要原因,其次常染色体的某些断裂点也可能影响精子发生。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物,利用PCR扩增方法,以beta-actin做内参,检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况,发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致,表明各模板DNA量基本一致;而在同一DNA浓度下,16S rDNA的扩增片段长度虽一致,但其产物量存在明显差异,精子16s rDNA产物量显著低于其他3种组织,其条带宽度和亮度很弱;16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

We discussed the effect of capacitation time on sperm motility in order to choose the most suitable time of in vitro insemination.

为了探讨精子获能时间对精子活力的影响,以选择适当的时间进行体外受精。

RESULTS: The sperm count and activity were increased significantly, and abnormality percentage was reduced in stress group.

结果: 应激组大鼠体重的增重较对照组降低,精子数量减少,精子活率降低,畸形率升高,血清睾酮含量降低(P均。01)。

Methods:Eight male adult beagel dogs were collected,6 of them were planted with IVD in their vas deferens and 2 as the control.

选雄性成年Beagle犬6只,手术植入IVD,术后1个月、3个月、6个月和12个月各采集犬精液,分别检测精子密度、精子活动力和精浆α-糖苷酶,并以仅做假手术的对照组(2只)作对照。

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