精子的

ConclusionThe formation and diminishment of the manchette is in step with the condensation and elongation of the nucleus of the spermatid. Both the structural and positional changes of the manchette coincide with the changes of the nucleus. These results imply that manchette might play an important role in mouse spermiogenesis.

Manchette结构的形成和消失与精子细胞核的浓缩及延伸同步，其形态变化和位置改变与精子细胞核的形态学变化相吻合，在小鼠精子细胞变态成形过程中具有重要意义。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：该基因在早期A型和B型精原细胞中不表达，从双线期初级精母细胞阶段开始表达并逐渐上调，在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA，但在成熟精子中消失，具有明显的阶段特异性表达特征，并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. It is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on.

除精子细胞的核蛋白不完全成熟及卵子激活不足等因素外，如何正确选择存活精子细胞是个难题，如圆形精子细胞与精母细胞、单核细胞和多形核白细胞等其他圆形细胞的区分就比较困难。

To investigate the relationship between spermatogenesis disorder and genetic defects of patients with azoospermia or severe oligospermia.

探讨无精子症和严重少精子症患者的遗传缺陷与精子生成障碍的关系。

Puberty when the hypothalamus pituitary development formed to stimulate the beginning of testicular gonadal hormone secretion, so that in the vas deferens spermatogonium split into spermatocytes, and then split into sperm cells, sperm cells eventually split into sperm.

青春期时，丘脑下部垂体的发育形成了，刺激睾丸性腺开始分泌性激素，使输精管里的精原细胞分裂成精母细胞，再分裂成精子细胞，最终精子细胞分裂成精子。

Spermatozoon generates: According to domestic and international data, The male 14 ～ is 15 years old, the number that spermatozoon makes and quality are general with manhood The male spermatozoon is basic and identical, they already had fecundity.

1精子生成：据国内外资料，男性14～15岁，精子生成的数目和质量一般都与成年男性的精子基本相同，他们已有了生育能力。

The semen analysis prior to treatment reealed that none of the men had normally shaped sperm and 213 of the men had subnormal sperm counts or sperm motility.

术前的精液分析显示所有人都没有正常形态的精子，且有213人精子计数和精子活性低于正常。

The ejaculates of three to four male goats, 2-3-year-old, were obtained by the artificial vagina method of collection with the aid of a female serving as dummy and then diluted with equal volume.

结果显示川东白山羊1岁时的阳性精子率为39.34%±13.76%，4岁时降为23.40%±19.37%，与1岁和2岁时相比，差异显著；南江黄羊的阳性精子率最高，波尔山羊最低；并筛选到一种山羊精子和外源DNA处理方法。

Methods Micronucleus test: Kuming mice were divided into 3 groups at the dibutyl phthalate dosage of 33,330,3 300 mg/kg,and the bone-narrow micronucleus test was undertaken according to the routine method.

选择妊娠期和哺乳期SD雌性大鼠，灌胃染毒，DBP剂量分别为0、125、250、500 mg/kg，计数F1代雄性大鼠的附睾尾精子数、精子活力、精子畸形率。结果DBP在3 300 mg/kg时能引起微核率的升高(P.01)。

MATERIAL AND METHODS: Thirty 30 healthy Sprague- Dawley adult male rats were randomly divided into 3 groups: control, alcohol 7.5 g/kg and alcohol 7.5 g/kg ＋ vitamin A 50 μ g/kg. Alcohol and vitamin A were administrated to the rats for 13 weeks by a gastric tube. The sperm counting ,motility and the percentage of abnormal sperm were observed. Serum testosterone was determined. The patholgical changes of testicle tissue of rats were observed by light and electron microscopy.

材料与方法： 30只健康雄性成年 SD 大鼠随机分为对照组、酒精组、酒精＋ VA 组，各组每日分别灌胃给予酒精0、7.5 g/kg 、酒精7.5 g/kg ＋ VA 50 μ g/kg ，连续13周后对各组大鼠的精子计数、精子活动率、精子畸形率、血清睾酮(Serum testosterone，T )进行检测，光、电镜观察睾丸生精上皮的组织学改变。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。