精子的

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状，由精子群、精荚基质及初级精荚壁和次级精荚壁组成，初级精荚壁与精荚基质是相连续的，它们之间无明显界限，两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成，但后者不含精子，后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物，可能与形成精子细胞核中的泡状带和膜状带有关，在交配后的雌虾，其头胸部腹面上粘着的精荚中，其基质内已不见絮状和同心圆泡，仅见稀疏的纤丝。

Methods Semen analyses were carried out according to the World Health Organization manual for 47 subfertile men,film preparation before and after centrifugate washing,then use HE dyeing,and assess the deformity ratio of sperm head,body,tail and SDI,TZI.

47例人精液标本进行常规检测密度及活力后，分别在离心前后涂片，应用快速苏木素-伊红染色法进行染色，检测精子头部、体部、尾部畸形率。对比精子畸形指数和畸形精子指数是否受到离心洗涤的影响。

Objective To evaluate the influence of serum follicle stimulating hormone, luteinizing hormone and testosterone levels to the type of azoospermia and the predictive value of inhibin B level as an indicator of the presence of testicular spermatozoa in patients with nonobstructive azoospermia.

目的 探讨血清促卵泡成熟激素、黄体生成素及睾酮等生殖激素水平对无精子症分型以及血清抑制素B水平对非梗阻性无精子症患者睾丸精子存在与否的预测价值。

Objective: Retrospective study of the results of ICSI(intracytoplasmic sperm insemination)with frozen sperm obtained by PESA( percutaneous epididymal sperm aspiration ) was performed in 27 patients.

目的：回顾性分析27例无精子症患者经皮附睾穿刺取精术所获精子冷冻复苏后行卵细胞胞质内单精子注射治疗后的效果及妊娠结局。

The sperm motility, the ultrastructure of spermatozoa and spermatogenesis were also studied.

并研究了斜带石斑鱼的精子活力，精子发生和精子超微结构。

Results A significant decrease of the number and density of sperms was seen in the experiment group as compared with the control group (P.01). In the experiment group the motility of sperm from caudal epididymides dropped to zero, and a large amount of teratosperms were found under micro-video camera.

与正常对照组比较，中药组大鼠附睾的精子计数和密度显著下降(P.01)，检测视频下可见大量畸形精子，精子活动力下降为零。

Methods The suspension of spermatozoa was obtained from cauda epididymides using "diffusion" method. Different doses of cadmium chloride (0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, 32.0 mg/L) were administrated to spermatozoa suspension.

方法］用扩散法收集大鼠附睾尾成熟精子制成精子悬液，分8组，CdCl2染毒浓度分别为0，0.5，1.0，2.0，4.0，8.0，16.0，32.0mg/L，每组6个平行样，染毒30min、1h、2h、4h后用CASA仪测定反映大鼠精子运动能力及运动方式的各项参数。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要：用冷冻保存的花鲈精子作为异源精子，采用紫外线对精子进行照射使其遗传物质失活，然后与卵子进行&授精&，可以刺激犬齿牙鲆鱼卵进行雌核发育，在受精后一定时间采用冷休克处理&授精&卵，抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。