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In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达,结果显示:在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达,而在成体的体壁组织、消化道组织、体腔球中则没有表达;在未受精卵中没有表达,而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达;在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

SRG-L protein wasmainly observed in diplotene/pachytene spermatocytes, round and elongating spermatids.We can conclude this gene was activated during the early stage of meiosis, andtranscription of it kept high level during spermiogenesis.

因此初步判断这个基因在减数分裂早期被激活,转录在整个精子形成期维持较高水平的表达,有可能在精子发生后期起特殊作用,与减数分裂和精子变态成形相关。

By using two color FISH with one euchromosome probe and one sex chromosome probe, the sperm with same color of two hybridization signals in one sperm head could be identified as aneuploidy sperm or diploid sperm.

若用1条常染色体探针和1条性染色体探针进行FISH,可以区别头部有2个相同颜色荧光杂交信号的精子属非整倍体精子或二倍体精子。

In normal condition, only one can inseminate oviduct.

人类男性的每一次射精,其中***所含的精子约有3亿5千个,能够顺利进入子宫内的精子则不足100万,到达输卵管的大约只有1000个左右,而有机会接近卵子的精子就只剩下100个而已,最后在正常情况下能获得授精机会的精子却只有一个。

Sperm nucleus immaturity rate in each AlCl3 group increased significantly (P.01) with a dose dependent manner.

精子核荧光染色试验中,高、中、低剂量AlCl3染毒组未成熟精子率均高于阴性对照组,差异有统计学意义(P.01);且未成熟精子率随着AlCl3染毒剂量的升高而增加。

Results Some spermatocytes all in model and clomiphere groups were denatured or death and spermatid did not develop.

结果:实验对照组的曲细精管上皮中多数精母细胞变性、死亡,精子细胞不发育,无精子形成,管腔中无精子,支持细胞有明显病变。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行"授精",可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理"授精"卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

Microdeletion of AZF gene is one of the causes leading to azoospermia and severe oligozoospermia, which has a close relation with the development of sperm defect.

AZF微缺失是引起特发性无精子症和重度少精子症的原因之一,与男性精子缺陷发生密切相关。

Methods Semen analyses were carried out according to the World Health Organization manual for 47 subfertile men,film preparation before and after centrifugate washing,then use HE dyeing,and assess the deformity ratio of sperm headbodytail and SDITZI.

47例人精液标本进行常规检测密度及活力后,分别在离心前后涂片,应用快速苏木素-伊红染色法进行染色,检测精子头部、体部、尾部畸形率。对比精子畸形指数和畸形精子指数是否受到离心洗涤的影响。

Sperm of Epididymides and ejaculation could bind tetramethylrhodamine-6-dUTP labeled exogeous DNA.

本实验还对附睾精子和射精精子结合外源DNA情况的比较,发现附睾精子与射精的精液经洗涤后的精子都能够吸附外源DNA,且二者的差异不显著。

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