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Results The total aberrantion rate of chromosome is 26.7%(23/86);Compared the normal controls,in azoospermic patients,Serum FSH、LH levels increased,The rate of T/LH decreased.and there is a very significant difference(P%26lt;0.05);The resemble results is detected in different outcome in PESA.

结果摘要:86例无精子症患者中异常核型23例,占总例数26.7%;和正常对照组相比较,无精子症组血清FSH、LH升高,T/LH比值下降,差异有显著性(P%26lt;0.05);PESA能抽吸到精子组比不能抽吸到精子组的FSH、LH偏高,T/LH下降(P%26lt;0.05)。

In the process of spermiogenesis, the proximal and distal centrioles translocate to the rear of the spermatid nucleus where the proximal centriole is attached, while the distal centriole elaborates the flagellum. The fluorescent centrin protein was seen to be located in basal body of flagellum. But the fluorescent spots could not be detected in the epididymal mature spermatozoa.

在精子变态分化过程中,近端和远端中心粒向核后方移动,近端中心粒附在核后膜上,远端中心粒伸出鞭毛,荧光显示Centrin蛋白位于伸出鞭毛的基部,但在成熟精子中该蛋白消失。

There were more Ca〓 deposite granules in and around the condensing granules of nucleoplasm; 2 There were large amount of Ca〓 in the Golgi complex of spermatid in Golgi phase. The Ca〓 in the acrosomal vesicle were probably from the Golgi complex; 3 Ca〓 was probably concerned with adherence of the acrosomal vesicle to the nuclear membrane.

发现(1)。Ca〓与精子形成过程中染色质的致密化有关,圆形精子细胞局部致密化的染色质团块周围及其内部和长形精子处于的致密化过程的核内均具有较多的Ca〓;(2)。

Two polar nuclei move to the micropylar end before sperm is released in a gap between the egg cell and central cell and subsequently taking off cytoplasm one after another. The sperm nucleus moving to the egg nucleus runs faster than the one to the polar nuclei. It fuses with the polar nuclei that are moving towards the antipodal cells at the chalazal end, Images showed the fusion process of male and female nuclei and polyspermous fertilization.

精子释放前,两极核移向卵细胞的合点端;两精子释放于卵细胞与中央细胞的间隙后,先后脱去细胞质,然后分别移向卵核和极核,移向卵核的精核快于移向极核的精核;精核与两极核在向反足细胞团方向移动的过程中完成雌雄核融合。

Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. It is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on.

除精子细胞的核蛋白不完全成熟及卵子激活不足等因素外,如何正确选择存活精子细胞是个难题,如圆形精子细胞与精母细胞、单核细胞和多形核白细胞等其他圆形细胞的区分就比较困难。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行"授精",可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理"授精"卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

A eupyrene sperm and an apyrene sperm combined together forming a normal mature sperm, and the function of apyrene sperm was helping eupyrene sperm to swim.

一个有核精子和一个无核精子联合在一起组成一个正常的成熟精子,其中无核精子的功能是帮助有核精子游动。

Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行&授精&,可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理&授精&卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

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