精原细胞

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Annexin A1 mainly distributed in nuclei of spermatogonia, acrosomal cap of spermatid and residual body of sperm.

免疫组织化学显示膜联蛋白A1主要分布于精原细胞核、精子细胞顶体帽及精子胞质残余体内。

The histosection of ram testes about 2-3 month old were made and observed, the morphology of germ cells were uniform in seminiferous tubules and did not enter the spermatogenesis. We have found a lot of PGP9.5 positive spermatogonium on basal membrane and it could be used culture in vitro.

对2~3月龄的山羊睾丸做切片观察，发现曲细精管中细胞形态比较单一，生精上皮还没有进入生精周期，通过免疫组化染色发现在曲细精管基底膜有大量PGP9.5 精原干细胞。

The bodies have much greater potential to regulate spermatogonial stem cell proliferation and differentiation. The dosage of GFRα1 mRNA produced by undifferential spermatogonial cells regulates the signal transduction of glial cell-derived neurotrophic factor so as to regulates the direction of spermato gonial stem cells: at a low level, spermatogonoial stem cell favors differentiation; at a high level, spermatogonial stem cell favors self-proliferation.

GFRαl在小鼠生精恢复过程中以剂量相关性调控著胶质细胞源性神经营养因数资讯传人，从而调控著精原干细胞的去向：高剂量时诱导其增殖，低剂量时诱导其分化。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果：苏木精－伊红染色细胞多为梭形，有伪足伸出，细胞核为圆形或椭圆形；甲苯胺蓝染色，胞浆为深蓝色；免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达；在体积分数为0.1的血清条件下，胰岛素样生长因子Ⅰ能提高细胞的增殖活性，并且在有效浓度范围内呈剂量效应关系。

Results The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.

结果 原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。

Objective: To construct cell model of spermatogonial stem cells with Sertoli cell layer in vitro, and study the proliferation characteristic of SSCs in vitro.

目的：建立体外研究精原干细胞与支持细胞共培养的细胞模型，探讨体外精原干细胞在支持细胞层上的增殖特点。

To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.

目的 以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。

In primary spermatogonia, mitochondria were larger and spherical, and contained few cristae.

在初级精原细胞阶段，线粒体较大，多为球形，嵴少，基质电子密度低。

Then the cells with low side scatter light-scattering properties were sorted and posit...

利用免疫荧光激活细胞分选术能分离出大量有活性的精原干细胞。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐