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In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫浆蛋白电泳谱带特性进行分析,使用酶联免疫方法检测浆血小板激活因子乙酰水解酶活性、化学比色法检测浆谷草转氨酶活性,并初步探讨了这些物质与大熊猫液质量的关系,结果显示:(1)大熊猫浆SDS-PAGE电泳共分离得到12条蛋白谱带,其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa,其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫浆样品所共有。

In accordance with the application of high\|eficacy comber,all processing targets and their relations were analysed,the importance of the processing technique matching with comber was analysed too,the application of high\|efficacy comber was pointed out,and some suggestions on technical reformation were give...

针对高效能梳机的使用,分析了高效能梳机各项工艺指标与相应关系和高效能梳机与工艺技术相配套的重要性;列举了高效能梳机的实际应用;提出了高效能梳机与技术改造的几点建议

Purified Water, Vegetable Emulsifying Wax, Almond Oil, Vegetable Glycerine, Cocoa Butter, Centella Extract Oil,Apricot Kernel Oil, Rosehip Oil, Wheatgerm Oil, Herm Seed Oil, Calendula Oil, Tocopheryl Acetate, Vitamin A Palmitate, Sodium Hydroxymethylglycinate,Borage Oil, 100% Pure Essential Oils of Rosewood, Mandarin, Geranium,Frankincense and Frangipani Absolute, Grapefruit Seed Extract

成分:纯净水、植物乳化蜡、杏仁油、植物甘油、可可油、积雪草萃取油、杏桃仁油、玫瑰籽油、小麦胚芽油、大麻籽油、金盏花浸泡油、维生素E醋酸盐、维生素A棕榈酸盐、Sodium Hydroxymethylglycinate,琉璃苣油、花梨木纯油,桔纯油、天竺葵纯油、乳香纯油,赤素馨花原、葡萄籽萃取

Take for instance the alternate gremlin who will fix during the battle your faulty golems – quite a handy man ! Or the skeleton footman who will complement the archer quite well, being more resilient and more aggressive. The elven sprites alternate upgrades have a use similar to the gremlins : they can heal your treants while healing themselves– you wont need them much until you get these treants, but they will be critical afterwards !

例如,升级的怪可以在战斗中修理损坏的傀儡(咳……这不是旧的升级么……),新的骷髅步兵可以很好的配合弓手,灵的新花可以使用类似怪的技能:她们可以治愈你的树并治愈她们自身-当你没有树的时候你可能不需要这些花,但是当你有树之后,她们将会成为影响胜利天平的临界砝码。

The histosection of ram testes about 2-3 month old were made and observed, the morphology of germ cells were uniform in seminiferous tubules and did not enter the spermatogenesis. We have found a lot of PGP9.5 positive spermatogonium on basal membrane and it could be used culture in vitro.

对2~3月龄的山羊睾丸做切片观察,发现曲细管中细胞形态比较单一,生上皮还没有进入生周期,通过免疫组化染色发现在曲细管基底膜有大量PGP9.5 原干细胞。

Conclu sion:(1)Surface densities of PCNApositive cells reached their peaks on 14th day after birth,but the number of apoptotic cells reached its peaks on 21st day p ostnatal,so the apoptotic peak was late the proliferating peak for a week,and related to the particular period in cycle of seminiferous epithelium;(2)The spe rmatogium were found move from center to the periphery of the seminiferous tubule and arrange disorder to order;(3)The spermatogonium remained replicating activ ity of DNA during the developing stage.

细胞凋亡的高峰要滞后其增殖峰1周左右,而且与其所处生周期的特定阶段有关;(2)原细胞在胚胎发育过程中向曲细管周边迁移,其排列由无序到有序;(3)在生后各发育阶段中,原细胞始终保持DNA复制的能力。

There exist in this world a range of sublunary spirits that carminibus coelo possunt deducere lunam, and they have been divided since ancient times into six kinds: fiery, aerial, terrestrial, watery, subterranean, and the whole class of fair-ies and nymphs.

世上有一群人间灵,carminibus coelo possunt deducere lunam 。它们早在远古时代就分成了六类:火、气、地、水、土,以及全体仙灵和水仙女。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-氨酸初始浓度对苯甲醛沉淀L-氨酸的影响,结果表明,沉淀反应的适宜pH值大于11,苯甲醛与L-氨酸的适宜摩尔比为1.25,(来源:Aa6BC论文网www.abclunwen.com)L-氨酸适宜的初始浓度在15g/L以上,氯化钠的存在对沉淀率基本上没有影响,而氯化铵和L-赖氨酸的存在使沉淀率下降;同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线,表明其最大平衡吸附量为30mg/g,考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响,结果显示从L-氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2,温度对L-赖氨酸吸附率影响不大,吸附过程可在室温下进行,氯离子的存在使L-赖氨酸的吸附率降低。

Face tonic with pure essential oils of lavender*, geranium, rosemary, ylang-ylang, verbena, mint, ratania root, pure spring water.

含有薰衣草油,天竺葵油,迷迭香油,依兰油,马鞭草油,薄荷油,ratania root油,纯泉水。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明:(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达,免疫组织化学结果显示COX-2定位于曲细管内的生细胞;正常成年男性睾丸组织中同样存在COX-2表达,免疫组织化学结果显示COX-2定位于曲细管内的生细胞和Leydig细胞;(2)服用特异性COX-2酶抑制剂rofecoxib 2周后,实验组大鼠睾丸组织内睾酮的含量减少,为正常对照组的50%;持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平;COX-2酶活性降低后病理组织切片显示睾丸内曲细管萎缩,生紊乱,持续用药4周时影响最明显;(3)注射特异性Leydig细胞杀灭剂EDS 2周后,实验组大鼠睾丸组织内睾酮浓度降至极低水平时,COX-2的蛋白和基因表达水平也显著低于正常空白对照组(P.005);使用EDS后第4周,睾丸组织中的睾酮浓度逐渐回升,同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平;使用EDS后4周开始给予外源性睾酮,6周和8周时COX-2表达水平的绝对值虽有提高,但与正常大鼠空白对照组比较并无显著性差异,说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。