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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

First observation of the essential oil of UVA (320-400nm) absorption, taking nine more before the absorption of essential oils and nine of the past record or doubt further analysis of essential oils, UVA nine large oil absorption of the former are: cinnamon, pheasant pepper, sweet orange, lemon, basil, grapefruit, bluegrass rock, roses, dill, 9 kinds of essential oils may be phototoxic as follows: orange blossom, bergamot, fennel, lemon grass, red mandarin, citronella oil, ginger oil, Australia sandalwood oil, lime; E.

首先观察精油的UVA(320-400nm)吸收度，取前九名有较大吸收之精油及九种过往记载或存疑之精油进一步分析，UVA较大吸收度之前九名精油分别为：肉桂、山鸡椒、甜橙、柠檬、罗勒、葡萄柚、岩兰草、玫瑰、莳萝，九种可能光毒精油为：橙花、佛手柑、茴香、柠檬草、红柑、香茅油、姜油、澳洲檀香油、莱姆；大肠杆菌抑菌圈变化中，柠檬、红柑及葡萄柚精油有明显的扩大现象；pUC119 DNA电泳结果，不同精油於电泳模式中，随UVA照光能量增加产生不等断裂情形，除玫瑰精油无影响，其余精油在UVA照光能量增加下皆有不等的DNA断裂情形发生，须再进一步试验。

The phylogenetic trees supported that Eriocaulaceae be treated as Eriocaulales because this family always been a nature independent group connected with other families. Eriocaulaceae grouped with Commelinaceae, which supported the close relationship and parallel evolution between the two families. Juncaceae was not close to Eriocaulaceae and more primitive. The relationship between Juncaceae and Cyperaceae was closer than that between Juncaceae and Poaceae. Among these families, Poaceae was the most primitive family and far from Eriocaulaceae. Also in Eriocaulaceae, Eriocaulon was supported to be more primitive.

从构建的系统发育树可以得到：①谷精草科独立形成一个植物类群，支持谷精草科独立成为谷精草目；②谷精草科与鸭跖草科聚成一个大的类群，说明两科的亲缘关系较近，并且表现为平行进化；③灯心草科不近缘于谷精草科，与禾本科相比，灯芯草科与莎草科之间存在更近的亲缘关系；④在进化程度上，灯心草科比谷精草科原始；禾本科与谷精草科的亲缘关系最远，并且在这几个科中处于最原始的进化地位；⑤系统树还支持谷精草科内部的系统关系，支持谷精草属在谷精草科中的原始系统位置。

The testis index, testis volumes were same as the annual changes of testis mass. The curves of annual variation were all unimodality.2 The spermatogenetic cycle of Myospalax cansus comprises seven stages with significant features: Stage I , from February to April, the testis were at the stage of spermatogonia proliferation. In this period, testis index and the number of spermatogonia began to rise. Other spermatogenic cells had not yet formed; Stage II to III, from March to April, primary spermatocyte meiosis period. The testis index was highest in this stage, and spermatogenic cells were in spermatocyte stage, the primary spermatocyte meiosis generated to secondary spermatocyte; Stage IV, from April to May, spermatocytes continued to split, germ cells appeared in seminiferous tubules; Stage V, in May, sperm formation, spermatids of seminiferous tubules were transformed to spermatozoa, a large number of sperms existed in the lumen; Stage VI, spermatozoa emission period, from May to June, testis index were a significant drop and mature spermatozoa excluded gradually; VII, the testicular activity ceased basically from July to September, November to January of the following year, the spermatogenic activity ceased completely. Therefore, Myospalax cansus are animals of seasonal reproduction, spermatogenesis cycle is discontinuous type.

睾丸系数、体积和重量的年周期变化规律一致，变化曲线呈现单峰型。2甘肃鼢鼠雄性生殖腺的年周期活动由7个特征明显的时期构成：Ⅰ期，2～3月份，精原细胞增殖期，睾丸系数开始上升，精原细胞进行有丝分裂，其他生精细胞尚未形成；3～4月份为Ⅱ～Ⅲ期，初级精母细胞成熟分裂期，睾丸系数达到最大，生精细胞大多处于精母细胞阶段，初级精母细胞减数分裂生成次级精母细胞；Ⅳ期，4～5月份精母细胞继续进行分裂，精细胞在生精小管内出现；Ⅴ期，5月份，精子形成期，曲细精管中精细胞变态成精子，在管腔中存在大量的精子；Ⅵ期，精子排放期，5～6月份，睾丸系数显著下降，成熟精子从生精小管上脱离，逐渐排除；Ⅶ期，精原细胞停滞期，7～9月份睾丸生精活动基本停滞，11～翌年1月，生精活动完全停止。

The apoptosis of 80 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocyte; the apoptosis of 120 mg/kg ﹒ d group was mainly located in spermatogenous cell , first spermatocyte and spermatocyte of the second order; the apoptosis of 240 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocytes, and seemingly, the apoptosis located in first spermatocyte was dominant; whereas the apoptosis of 480 mg/kg ﹒ d group was located in all levels spermatogenic cells.

结果表明：各试验组小鼠生精细胞凋亡指数随着浓度的升高有增加的趋势，而且与对照组差异显著（ P ＜0.05或 P ＜0.01）。80 mg/kg · d 组凋亡细胞主要定位于精原细胞和初级精母细胞；120 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞和次级精母细胞；240 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞，以初级精母细胞为最多；而480 mg/kg · d 组凋亡细胞定位于各级生精细胞。

GOD/GODDESS/ALL THAT IS Essential Oil: Bergamot 48、神/女神/一切万有精油:佛手柑 Bergamot is a lovely citrus oil that is also used in the making of Earl Grey Tea.

佛手柑是一种可爱的柑橘类精油，也用于制作格雷伯爵茶。

Lo Han Sheng Xuanhuai supervision is a马屁精, specially prepared to a float, car bed one side, there is a door called "wishful barrels," the toilet.

芦汉督办盛宣怀是个马屁精，特地准备一辆花车，车内床侧有一门，门内有一被称作&如意桶&的马桶。

INTERNAL Essential Oil: Cardamom 22、内在精油:小豆蔻 Cardamom is an oil who's scent will open you up to your inner world or dream time to better discern your own truth in the dance of life.

小豆蔻精油的香味将你打开到你的内在世界或梦想时间，以更好甄别你自己生命之舞中的真相。

The my plant profession produces "精忠" brand tin system handicraft product, the product category has tea vessel, wine to have, the craft put a piece, pure tin of such as pure tin tea caddy, wine pot, teapot, vase, container for pens, medal and prize dish etc., the new product has the tin system the crystal tea caddy, widely popular.

本厂专业生产&精忠&牌锡制工艺品，产品类别有茶具、酒具、工艺摆件，纯锡的如纯锡茶叶罐、酒壶、茶壶、花瓶、笔筒、奖牌和奖盘等，新品有锡制水晶茶叶罐，广受欢迎。

HONOR Essential Oil: Hyssop 31、尊重精油:海索草 Hyssop is an oil useful to the regeneration of the skin and is a good addition to the bath, homemade lotions or body oils as a result.

海索草是一种对皮肤再生有益的精油，故而也是一种加入沐浴、自制护肤液或护肤油中的很好物质。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷