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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

A highly flammable, colorless or yellowish syrupy solution of pyroxylin, ether, and alcohol, used as an adhesive to close small wounds and hold surgical dressings, in topical medications, and for making photographic plates.

火棉胶一种极易燃的、无色或黄色糖浆状火棉、乙醚和酒精混合溶液,用于局部药物,治疗中粘涂愈治小的创伤和及附外科包扎,并可制作底片

In an accompanying editorial, Loren Laine, from the University of Southern California School of Medicine in Los Angeles, notes the marked variability between studies of adherent clots.

在一篇附论中,洛杉矶南加利福尼亚大学医学院的Loren Laine指出,粘著性血凝块的研究中存在著明显的可变性。

With the microelectromechanical technique, we developed the taste cell chip based on LAPS, the surface of which had already been soaked in the phosphate-buffered saline with 100μg/ml poly-L-ornithine and 8μg/ml laminin to enhance the attachment of cell on it.

采用微机械加工技术,我们制备了LAPS味觉细胞芯片,并用多聚鸟氨酸和层粘素混合的磷酸缓冲液处理以增强细胞在芯片表面的黏附性。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?