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Methods: Immunohistochemical method was used to observe the distribution of LN and FN in normal chorion, hyditidiform mole and choriocarcinoma.

用免疫组织化学方法观察层粘连蛋白和纤维粘连蛋白在正常绒毛膜、葡萄胎和绒毛膜癌组织中的表达。

Objective To explore the effects of LN and FN in the process of development of villi, and formation of placenta.

层粘连蛋白和纤维粘连蛋白(Fibronectin,FN)是细胞外基质的重要组成成分。

Surgical therapy:(1) Laparoscopy: Decomposition of adhesion,电灶lesions, puncture of endometrial Xiang swelling, swollen Xiang stripping (2) laparotomy conservative surgery: separation of pelvic adhesions, fulguration or knife cut out a visible lesion, HoLEP out Xiang endometrial swelling, as far as possible, to retain normal ovarian tissue.

口服3-5月。3、手术疗法:(1)腹腔镜:分解粘连,电灶病灶,穿刺内膜襄肿,襄肿剥离(2)剖腹保守术:分离盆腔粘连,电灼或手术刀剜出可见病灶,剜出内膜襄肿,尽可能保留正常的卵巢组织。

Objective:To investigate the feasibility and efficacy of adhesive ileus treated by laparoscope.

目的:探讨腹腔镜下肠粘连松解术治疗粘连性肠梗阻的可行性及疗效。

To study the effect of laminin and fibronectin in the process of implantation.

目的:为探讨层粘连蛋白和纤维粘连蛋白在胚泡着床过程中的生物学作用。

A rat model of intestinal adhesions was done as follows: serous membrane of the caecum was scuffed, its raw surface was dropped with absolute alcohol, the mesentric artery of the caecum was then clamped.

外科盆腹腔术后肠粘连一直是临床工作中的一大难题,如顽固性腹痛、不育及粘连性肠梗阻等,后者如不及时处理将导致腹腔炎、水电解质紊乱甚至危及生命。

Results Abdominally retained crumpled gauze was instantly adhesive with omentum and neighboring intestines tightly. Fibro-connective tissue membrane was produced at the adhesion site gradually, spreading out to enclose the crumpled gauze.

结果 遗留腹内的纱布团立即被大网膜及邻近肠管粘连包裹,自肠管与纱布团粘连处向四周逐渐扩展生成纤维结缔组织包膜,约3~4周时间逐步将整个纱布团完全包绕,并向纱布团网眼内生长增厚。

Resolution of infection may be followed by adhesive arachnoiditis with obliteration of subarachnoid space leading to obstructive hydrocephalus.

感染的溶解可能引起粘连性蛛网膜炎,粘连性蛛网膜炎使蛛网膜下腔变小甚至消失,最后造成梗阻性脑积水。

Fallopian tube pass for the remote and poor and there is no good treatment, if the fallopian tube adhesion umbrella serious side can do laparoscopic or open around the stoma and the separation of adhesion, if it is slightly remote and poor pass, Our experience is that too much to take the treatment, this is because the poor pass that mild lesions less, the purpose of treating disease is the history of lighter, but there are many ways to present a certain degree of injury is likely to therapy not as good as dead after some hospitals that laparoscopic treatment, we believe that is not suitable, because there is trauma laparoscopy and laparoscopic surgery to stop bleeding in the separation of mainly electrocoagulation, fallopian tube ignition organizations that can be damaged, causing new inflammation, resulting in tubal adhesions client umbrella.

对于输卵管远端通而不畅并没有什么好的治疗方法,如果输卵管伞端粘连严重的话可以在腹腔镜下或开腹做造口及分离周围粘连,如果是远端轻度通而不畅,我们的经验是不主张采取过多的治疗,这是因为轻度通而不畅说明病变比较轻,治疗的目的是是史病变更轻一些,但是目前的很多方法都有一定的损伤很可能治疗后还不如不治,有的医院主张腹腔镜治疗,我们认为不适合,因为腹腔镜有创伤,而且腹腔镜术中的止血分离主要是电凝,灼烧这都可能使输卵管组织遭到破坏,引起新的炎症,造成输卵管伞端粘连。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果:(1)层粘连蛋白组处于G_2~M期细胞比例较对照组显著提高,Go~G_1期细胞比例显著下降,提示层粘连蛋白促进内皮细胞DNA合成,及细胞分裂增殖;光镜下,对照组细胞分布成团状,细胞密度较低,细胞间连接紧密,细胞内微丝结成网状,细胞核大小一致;与对照组相比,层粘连蛋白组细胞生长旺盛,细胞密度高,向周边伸出大量板状及丝状伪足,细胞内微丝增多、拉长、集结成束,伸入伪足中,细胞核形状大小不一致;(2)倒置显微镜观察及细胞生长曲线显示,各组细胞数目随时间增加而明显增多,各实验组较对照组增生显著,EGF和LN联合应用组各时间点细胞数目最高;实验组处于S期和G_2~M期细胞数目增加,Go~G_1期细胞数目减少;提示EGF、LN单独及联合应用均可促进细胞增殖,但尚不能认为二者有交互作用;(3)倒置显微镜下,组织培养的猫角膜内皮细胞排列成密集的单层,细胞间连接紧密;组织学观察发现,培养的猫角膜内皮细胞形成完整的内皮层,贴附于脱水基质的后弹力膜上,与正常的角膜内皮组织结构相似;扫描电镜下,组织培养的猫角膜内皮细胞间紧密镶嵌排列,可见某些细胞呈近似六边形排列,细胞大小不甚一致,胞核清晰。

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